Opendata, web and dolomites

NaKStruc SIGNED

Structural studies of Na,K-ATPase isoforms and mutants

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 NaKStruc project word cloud

Explore the words cloud of the NaKStruc project. It provides you a very rough idea of what is the project "NaKStruc" about.

lay    neuronal    heart    health    astrocytes    cycle    na    cardiac    selectively    disorder    neurological    reaction    ions    beta    extracellular    microscopy    crystallography    keep    resting    expressed    voltage    electron    heterooligomer    exhibit    neural    severe    muscle    remained    purified    regulators    disease    pastoris    cryo    isoform    dependence    elevations    root    elusive    mutations    hemiplegia    complexes    hence    physiology    pharmacological    glaucoma    details    atpase    subunit    diseases    inactive    inactivate    steroids    childhhod    e815k    significantly    causing    steeper    transient    attributed    gap    body    alternating    alpha    differences    molecular    ciliary    inhibited    affinity    physiological    pump    pichia    structure    treatment    dark       yeast    ray    reactions    cognitive    drug    isoforms    housekeeping    subsequently    foundation       drugs    lower    partial    inactivation    motor    structural    primary    d801n    effect    leaving   

Project "NaKStruc" data sheet

The following table provides information about the project.

Coordinator
AARHUS UNIVERSITET 

Organization address
address: NORDRE RINGGADE 1
city: AARHUS C
postcode: 8000
website: www.au.dk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Denmark [DK]
 Total cost 200˙194 €
 EC max contribution 200˙194 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2017
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-06-01   to  2020-05-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    AARHUS UNIVERSITET DK (AARHUS C) coordinator 200˙194.00

Map

 Project objective

Isoform complexes of Na,K-ATPase , an αβ-heterooligomer, have recently been identified as crucial regulators of muscle and neural physiology and were furthermore identified as the primary cause of neurological diseases. Of particular interest are α2β2 & α2β3, which are expressed in muscle and astrocytes (α2β2) and the ciliary body (α2β3). Both exhibit a significantly lower affinity for K-ions and a steeper voltage dependence than the housekeeping α1β1 complex. These features keep α2-isoforms inactive at resting conditions but allow to respond to transient elevations of extracellular K after muscle and neuronal activity. Moreover, both complexes can be selectively inhibited by cardiac steroids, making them promising drug targets for the treatment of heart failure (α2β2) and glaucoma (α2β3). Differences in affinity for K and cardiac steroids were attributed to the β-subunit recently, yet the root cause and molecular details remained elusive. Hence, I propose to investigate the structure of α2-isoforms purified from the yeast Pichia pastoris by x-ray crystallography and cryo electron microscopy. In addition to its physiological and pharmacological role mutations of Na,K-ATPase cause severe neurological diseases, e.g. Alternating Hemiplegia of Childhhod. Disease mutations typically inactivate the Na-pump causing severe motor and cognitive disorder. However, the effects of mutations on the ATPase’s reaction cycle have not been investigated in detail, leaving the cause of inactivation in the dark. Part two of the proposal addresses this gap of knowledge. The two most common mutations E815K and D801N will be expressed in P. pastoris and the effect of mutations on partial reactions of the Na,K-ATPase’s reaction cycle will be investigated and subsequently, structural studies will be carried out. This work will promote our understanding of Na,K-ATPase in health and disease and lay the foundation for the development of new drugs.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "NAKSTRUC" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "NAKSTRUC" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

Photonic Radar (2019)

Implementation of Long Reach Hybrid Photonic Radar System and convergence over FSO and PON Networks

Read More  

CORRELATION (2020)

Characterization and prediction of service-level traffic for future sliced mobile network

Read More  

LICONAMCO (2019)

Light-controlled nanomagnetic computation schemes

Read More