Opendata, web and dolomites

Autophagy in vitro SIGNED

Reconstituting Autophagosome Biogenesis in vitro

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 Autophagy in vitro project word cloud

Explore the words cloud of the Autophagy in vitro project. It provides you a very rough idea of what is the project "Autophagy in vitro" about.

larger    organelles    vegetative    pathophysiology    autoimmune    tirf    cells    organization    structure    sealing    fluorescent    confocal    atomic    cytoplasmic    cytotoxic    capture    found    forms    serves    catabolic    humans    material    atg16    mechanistic    membranes    vivo    stresses    cultured    shaped    tecpr1    yeast    unravel    components    conjugating    double    seven    effort    cup    lysosomes    ubiquitin    vitro    functional    atg16l1    atg    electron    labeled    possess    human    productive    proteins    microscopy    variants    reconstituted    gives    diseases    cargo    differences    atg5    atg12    autophagy    recombinant    interconnected    conjugation    canonical    degradation    delivers    scaffold    insights    coordinates    model    superfluous    starvation    exclusive    force    mutually    belief    expands    atg8    induces    homologs    synthetic    ub    spatiotemporal    expansion    unselectively    function    membrane    tested    aggregates    engulf    damaged    neurodegenerative    cancer    autophagosomes    quality    surrounded    coordinate    structural    protein    isolation    revealing    precursor   

Project "Autophagy in vitro" data sheet

The following table provides information about the project.

Coordinator
INSTITUT PASTEUR 

Organization address
address: RUE DU DOCTEUR ROUX 25-28
city: PARIS CEDEX 15
postcode: 75724
website: http://www.pasteur.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Total cost 1˙499˙726 €
 EC max contribution 1˙499˙726 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2014-STG
 Funding Scheme ERC-STG
 Starting year 2015
 Duration (year-month-day) from 2015-04-01   to  2020-09-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    INSTITUT PASTEUR FR (PARIS CEDEX 15) coordinator 1˙185˙057.00
2    MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV DE (MUENCHEN) participant 314˙668.00

Map

 Project objective

Autophagy is a catabolic pathway that delivers cytoplasmic material to lysosomes for degradation. Under vegetative conditions, the pathway serves as quality control system, specifically targeting damaged or superfluous organelles and protein-aggregates. Cytotoxic stresses and starvation, however, induces the formation of larger autophagosomes that capture cargo unselectively. Autophagosomes are being generated from a cup-shaped precursor membrane, the isolation membrane, which expands to engulf cytoplasmic components. Sealing of this structure gives rise to the double-membrane surrounded autophagosomes. Two interconnected ubiquitin (Ub)-like conjugation systems coordinate the expansion of autophagosomes by conjugating the autophagy related (Atg)-protein Atg8 to the isolation membrane. In an effort to unravel the function of Atg8, we reconstituted the system on model membranes in vitro and found that Atg8 forms together with the Atg12–Atg5-Atg16 complex a membrane scaffold which is required for productive autophagy in yeast. Humans possess seven Atg8-homologs and two mutually exclusive Atg16-variants. Here, we propose to investigate the function of the human Ub-like conjugation system using a fully reconstituted in vitro system. The spatiotemporal organization of recombinant fluorescent-labeled proteins with synthetic model membranes will be investigated using confocal and TIRF-microscopy. Structural information will be obtained by atomic force and electron microscopy. Mechanistic insights, obtained from the in vitro work, will be tested in vivo in cultured human cells. We belief that revealing 1) the function of the human Ub-like conjugation system in autophagy, 2) the functional differences of Atg8-homologs and the two Atg16-variants Atg16L1 and TECPR1 and 3) how Atg16L1 coordinates non-canonical autophagy will provide essential insights into the pathophysiology of cancer, neurodegenerative, and autoimmune diseases.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "AUTOPHAGY IN VITRO" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "AUTOPHAGY IN VITRO" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

EVOCELFATE (2019)

Evolution of cell fate specification modes in spiral cleavage

Read More  

ENTRAPMENT (2019)

Septins: from bacterial entrapment to cellular immunity

Read More  

SmartForests (2020)

Smart Forests: Transforming Environments into Social-Political Technologies

Read More