Opendata, web and dolomites


The unique dioxygenases of phytophagous spider mites: new enzyme players in plant-herbivore interactions?

Total Cost €


EC-Contrib. €






 DOGMITE project word cloud

Explore the words cloud of the DOGMITE project. It provides you a very rough idea of what is the project "DOGMITE" about.

acquired    mite    genome    cutting    codes    arthropod    dissect    drosophila    structures    enzymes    transfer    act    uncovered    advantage    ring    functionally    layered    transforming    selective    fungal    map    elucidate    transformation    extend    xenobiotics    insect    strategy    unbiased    reaction    proliferated    detoxification    counterplay    families    polyphagous    cleave    dioxygenases    differential    coli    meticulously    edge    agriculture    biotechnological    family    tetranychus    animals    herbivores    cells    substrates    genomes    counteract    metabolomics    create    herbivore    extremely    situ    preliminary    secondary    biological    cleaving    xenobiotic    aromatic    experiments    polyphenol    pesticides    found    picture    horizontal    urticae    expression    arthropods    gene    donor    intradiol    class    defense    oxidases    genetically    avenues    phytophagous    metabolites    unravel    time    plan    enzyme    showed    performed    poisonous    mites    first    functional    plant    guided    feeding   

Project "DOGMITE" data sheet

The following table provides information about the project.


Organization address
address: SPUI 21
postcode: 1012WX

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Netherlands [NL]
 Project website
 Total cost 165˙598 €
 EC max contribution 165˙598 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2015
 Duration (year-month-day) from 2015-09-01   to  2017-08-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

Detoxification of poisonous xenobiotics in animals is typically performed by multi-gene enzyme families. Within arthropods, only insect genomes have been studied in detail where these families are well characterized. I recently uncovered a new enzyme family in the genome of a non-insect arthropod, the extremely polyphagous plant-feeding mite Tetranychus urticae and showed that this proliferated family was acquired via horizontal gene transfer from a fungal donor. The family codes for intradiol ring cleaving dioxygenases which cleave a particular set of aromatic structures, commonly found in pesticides and plant metabolites. Here, I propose to functionally characterize this exciting new gene family and to elucidate its role in xenobiotic detoxification, with a focus on plant secondary metabolites. First, to create a general picture, I will map the in situ expression of dioxygenases and time their responses to plant-derived secondary defense metabolites. Second, guided by preliminary results, I plan to study how these new herbivore enzymes counteract polyphenol oxidases, well-known plant defense enzymes that act against herbivores and target the same class of substrates. State-of-the-art plant transformation experiments will be performed in order to meticulously dissect their counterplay. Finally, by means of an unbiased multi-layered strategy, I will functionally characterize these new dioxygenases. Dioxygenases will be introduced into biological systems by functional expression in E. coli or insect cells, and by genetically transforming Drosophila. Cutting-edge differential metabolomics will identify the substrates and reaction products. By means of this project, I expect to unravel the selective advantage of this new family for phytophagous mites and open up avenues to exciting biotechnological applications which I expect to extend well beyond agriculture.


year authors and title journal last update
List of publications.
2016 Wim Jonckheere, Wannes Dermauw, Vladimir Zhurov, Nicky Wybouw, Jan Van den Bulcke, Carlos A. Villarroel, Robert Greenhalgh, Mike Grbić, Rob C. Schuurink, Luc Tirry, Geert Baggerman, Richard M. Clark, Merijn R. Kant, Bartel Vanholme, Gerben Menschaert, Thomas Van Leeuwen
The salivary protein repertoire of the polyphagous spider mite Tetranychus urticae : a quest for effectors
published pages: mcp.M116.058081, ISSN: 1535-9476, DOI: 10.1074/mcp.M116.058081
Molecular & Cellular Proteomics 2019-06-14
2016 Nicky Wybouw, Yannick Pauchet, David G. Heckel, Thomas Van Leeuwen
Horizontal gene transfer contributes to the evolution of arthropod herbivory
published pages: evw119, ISSN: 1759-6653, DOI: 10.1093/gbe/evw119
Genome Biology and Evolution 2019-06-14
2017 Gonçalo Santos-Matos, Nicky Wybouw, Nelson E. Martins, Flore Zélé, Maria Riga, Alexandre B. Leitão, John Vontas, Miodrag Grbić, Thomas Van Leeuwen, Sara Magalhães, Élio Sucena
Tetranychus urticae mites do not mount an induced immune response against bacteria
published pages: 20170401, ISSN: 0962-8452, DOI: 10.1098/rspb.2017.0401
Proceedings of the Royal Society B: Biological Sciences 284/1856 2019-06-14
2017 Nena Pavlidi, Anastasia Gioti, Nicky Wybouw, Wannes Dermauw, Michael Ben-Yosef, Boaz Yuval, Edouard Jurkevich, Anastasia Kampouraki, Thomas Van Leeuwen, John Vontas
Transcriptomic responses of the olive fruit fly Bactrocera oleae and its symbiont Candidatus Erwinia dacicola to olive feeding
published pages: 42633, ISSN: 2045-2322, DOI: 10.1038/srep42633
Scientific Reports 7 2019-06-14
2017 Caleb R. Schlachter, Vincent Klapper, Nicky Wybouw, Taylor Radford, Thomas Van Leeuwen, Miodrag Grbic, Maksymilian Chruszcz
Structural Characterization of a Eukaryotic Cyanase from Tetranychus urticae
published pages: 5453-5462, ISSN: 0021-8561, DOI: 10.1021/acs.jafc.7b01333
Journal of Agricultural and Food Chemistry 65/27 2019-06-14
2017 Heike Staudacher, Bernardus Schimmel, Mart Lamers, Nicky Wybouw, Astrid Groot, Merijn Kant
Independent Effects of a Herbivore’s Bacterial Symbionts on Its Performance and Induced Plant Defences
published pages: 182, ISSN: 1422-0067, DOI: 10.3390/ijms18010182
International Journal of Molecular Sciences 18/1 2019-06-14

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