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KREDs in GSBs

Directed evolution of ketoreductases in gel-shell beads

Total Cost €

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EC-Contrib. €

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Partnership

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 KREDs in GSBs project word cloud

Explore the words cloud of the KREDs in GSBs project. It provides you a very rough idea of what is the project "KREDs in GSBs" about.

industrial    ketoreductase    broadening    skill    gel    derivatives    series    host    secondary    directed    substrate    ketones    scaled    alcohols    nad    chemistry    enzymes    horizon    selective    concentrations    communicated    limiting    manufacture    macromolecular    ideal    trials    screening    campaigns    reactor    group    kreds    industry    precise    glaxosmithkline    retained    individual    matching    cambridge    training    throughput    agarose    excellent    solvent    professional    corresponding    pharmaceutical    secondment    shell    12    cytometry    exchanged    gsk    tolerance    evolution    transfer    green    enantioselectivity    meet    readily    public    performed    members    bed    intensive    engagements    molecules    career    opening    solution    beads    always    detection    assays    endeavour    reaction    maintained    final    university    2020    efficient    courses    contact    ketoreductases    surrounded    791    optically    affords    continuous    2014    laboratory    small    size    forefront    pure    previously    aid    nature    library    alcohol    beneficiary    biocatalysis    flow    cofactor    sensitive    enzyme   

Project "KREDs in GSBs" data sheet

The following table provides information about the project.

Coordinator
THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE 

Organization address
address: TRINITY LANE THE OLD SCHOOLS
city: CAMBRIDGE
postcode: CB2 1TN
website: www.cam.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Project website http://www.bioc.cam.ac.uk/hollfelder/members/laurens
 Total cost 183˙454 €
 EC max contribution 183˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2015
 Duration (year-month-day) from 2015-06-01   to  2017-05-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE UK (CAMBRIDGE) coordinator 183˙454.00

Map

 Project objective

Optically pure secondary alcohols are required in the manufacture of many pharmaceutical products. Ketoreductases (KREDs) capable of reduction of ketones to the corresponding secondary alcohol have been improved through classical directed evolution campaigns but a limiting factor has always been the challenge of matching selection conditions to the final scaled-up reaction. Here we propose a solution to that problem. The Host group previously described the formation of gel-shell beads, agarose beads surrounded by a size-selective shell (Nature Chemistry 2014, 6:791). The beads allow screening of individual members of an enzyme library using flow cytometry. As small molecules can be readily exchanged, while enzymes are retained, the process not only affords precise control over selection conditions but also makes it ideal for continuous flow processes. To support this endeavour, sensitive assays for the high throughput detection of ketoreductase activity and enantioselectivity will be developed. Directed evolution will then be performed with selection for the efficient use of macromolecular derivatives of the NAD(P)H cofactor and improved tolerance to high substrate /solvent concentrations. Throughout the project, intensive contact will be maintained with Industrial Partner GlaxoSmithKline, leading up to flow bed reactor trials during a secondment. This project will provide the Beneficiary with an excellent training in biocatalysis, broadening his skill set and opening up new career opportunities in Europe’s growing Green Chemistry Sector. The Host laboratory is at the forefront of directed evolution of enzymes. Through the secondment at GSK, the Beneficiary will be able to transfer this technology to industry. Excellent training courses offered by the University of Cambridge will further aid his professional development. This project seeks to help Europe meet Part 12 of Horizon 2020, which is to be communicated through a well-planned series of Public Engagements.

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