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BrightSens SIGNED

Ultrabright Turn-on Fluorescent Organic Nanoparticles for Amplified Molecular Sensing in Living Cells

Total Cost €

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EC-Contrib. €

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Partnership

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 BrightSens project word cloud

Explore the words cloud of the BrightSens project. It provides you a very rough idea of what is the project "BrightSens" about.

surface    assembly    detection    free    brightsens    internalization    gain    quenching    switching    core    living    dye    apoptosis    packages    first    membrane    hydrophobic    brightness    sensing    whereas    nanoparticles    multidisciplinary    resolve    intracellular    fons    markers    ensembles    nanoprobes    photochemical    designed    existing    cultures    bulky    photophysical    synthesis    transfer    visualization    fluorescent    cytosol    3d    dyes    individual    switch    unable    turn    imaging    forster    collective    precise    cells    inside    2d    organic    counterions    micellar    receptors    energy    colour    harvest    encapsulated    barriers    inorganic    bright    fundamental    biodegradable    self    original    biomolecules    cellular    cooperative    resonance    molecular    sensor    originally    disruption    background    limited    mrna    doped    risk    light    organization    cancer    cell    polymer    trigger    nanomaterials    complete    convert    gt    undergo    molecule    off    ultrabright    amplified    probes    acceptor    single    entry    stimuli   

Project "BrightSens" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITE DE STRASBOURG 

Organization address
address: RUE BLAISE PASCAL 4
city: STRASBOURG
postcode: 67081
website: http://www.unistra.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Project website http://www-lbp.unistra.fr/rubrique78.html
 Total cost 1˙999˙750 €
 EC max contribution 1˙999˙750 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2014-CoG
 Funding Scheme ERC-COG
 Starting year 2015
 Duration (year-month-day) from 2015-06-01   to  2020-05-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITE DE STRASBOURG FR (STRASBOURG) coordinator 1˙999˙750.00

Map

 Project objective

Existing fluorescent molecular probes, due to limited brightness, do not allow imaging individual biomolecules directly in living cells, whereas bright fluorescent nanoparticles are unable to respond to single molecular stimuli and their inorganic core is not biodegradable. The aim of BrightSens is to develop ultrabright fluorescent organic nanoparticles (FONs) capable to convert single molecular stimuli into collective turn-on response of >100 encapsulated dyes, and to apply them in amplified molecular sensing of specific targets at the cell surface (receptors) and in the cytosol (mRNA). The project is composed of three work packages. (1) Synthesis of FONs: Dye-doped polymer and micellar FONs will be obtained by self-assembly. Molecular design of dyes and the use of bulky hydrophobic counterions will enable precise control of dyes organization inside FONs, which will resolve the fundamental problems of self-quenching and cooperative on/off switching in dye ensembles. (2) Synthesis of nanoprobes: Using cooperative Forster Resonance Energy Transfer from FONs to originally designed acceptor-sensor unit, we propose synthesis of the first nanoprobes that (a) undergo complete turn-on or colour switch in response to single molecular targets and (b) harvest light energy into photochemical disruption of cell membrane barriers. (3) Cellular applications: The obtained nanoprobes will be applied in 2D and 3D cultures of cancer cells for background-free single-molecule detection of membrane receptors and intracellular mRNA, which are important markers of cancer and apoptosis. An original concept of amplified photochemical internalization is proposed to trigger by light entry of nanoprobes into the cytosol. This high-risk/high-gain multidisciplinary project will result in new organic nanomaterials with unique photophysical properties that will enable visualization of biomolecules at work in living cells with expected impact on cancer research.

 Publications

year authors and title journal last update
List of publications.
2018 Tkhe Fam, Andrey Klymchenko, Mayeul Collot
Recent Advances in Fluorescent Probes for Lipid Droplets
published pages: 1768, ISSN: 1996-1944, DOI: 10.3390/ma11091768
Materials 11/9 2020-02-19
2019 Xinyue Wang, Nicolas Anton, Pichandi Ashokkumar, Halina Anton, Tkhe Kyong Fam, Thierry Vandamme, Andrey S. Klymchenko, Mayeul Collot
Optimizing the Fluorescence Properties of Nanoemulsions for Single Particle Tracking in Live Cells
published pages: 13079-13090, ISSN: 1944-8244, DOI: 10.1021/acsami.8b22297
ACS Applied Materials & Interfaces 11/14 2020-02-19
2018 Redouane Bouchaala, Ludovic Richert, Nicolas Anton, Thierry F. Vandamme, Smail Djabi, Yves Mély, Andrey S. Klymchenko
Quantifying Release from Lipid Nanocarriers by Fluorescence Correlation Spectroscopy
published pages: 14333-14340, ISSN: 2470-1343, DOI: 10.1021/acsomega.8b01488
ACS Omega 3/10 2020-02-19
2020 Nina Melnychuk, Sylvie Egloff, Anne Runser, Andreas Reisch, Andrey S Klymchenko
Light-harvesting nanoparticle probes for FRET-based detection of oligonucleotides with single-molecule sensitivity
published pages: , ISSN: 1433-7851, DOI: 10.1002/anie.201913804
Angewandte Chemie International Edition 2020-02-19
2019 Mayeul Collot, Pichandi Ashokkumar, Halina Anton, Emmanuel Boutant, Orestis Faklaris, Thierry Galli, Yves Mély, Lydia Danglot, Andrey S. Klymchenko
MemBright: A Family of Fluorescent Membrane Probes for Advanced Cellular Imaging and Neuroscience
published pages: 600-614.e7, ISSN: 2451-9456, DOI: 10.1016/j.chembiol.2019.01.009
Cell Chemical Biology 26/4 2020-02-19
2019 Pichandi Ashokkumar, Anila Hoskere Ashoka, Mayeul Collot, Amitava Das, Andrey S. Klymchenko
A fluorogenic BODIPY molecular rotor as an apoptosis marker
published pages: 6902-6905, ISSN: 1359-7345, DOI: 10.1039/c9cc03242h
Chemical Communications 55/48 2020-02-19
2020 Farah Bouhedda, Kyong Tkhe Fam, Mayeul Collot, Alexis Autour, Stefano Marzi, Andrey Klymchenko, Michael Ryckelynck
A dimerization-based fluorogenic dye-aptamer module for RNA imaging in live cells
published pages: 69-76, ISSN: 1552-4450, DOI: 10.1038/s41589-019-0381-8
Nature Chemical Biology 16/1 2020-02-19
2019 Nagappanpillai Adarsh, Andrey S. Klymchenko
Ionic aggregation-induced emission dye with bulky counterions for preparation of bright near-infrared polymeric nanoparticles
published pages: 13977-13987, ISSN: 2040-3364, DOI: 10.1039/c9nr04085d
Nanoscale 11/29 2020-02-19
2018 Nina Melnychuk, Andrey S. Klymchenko
DNA-Functionalized Dye-Loaded Polymeric Nanoparticles: Ultrabright FRET Platform for Amplified Detection of Nucleic Acids
published pages: 10856-10865, ISSN: 0002-7863, DOI: 10.1021/jacs.8b05840
Journal of the American Chemical Society 140/34 2020-02-19
2019 Mayeul Collot, Sophie Bou, Tkhe Kyong Fam, Ludovic Richert, Yves Mély, Lydia Danglot, Andrey S. Klymchenko
Probing Polarity and Heterogeneity of Lipid Droplets in Live Cells Using a Push–Pull Fluorophore
published pages: 1928-1935, ISSN: 0003-2700, DOI: 10.1021/acs.analchem.8b04218
Analytical Chemistry 91/3 2020-02-19
2019 Anne Runser, Denis Dujardin, Pauline Ernst, Andrey S. Klymchenko, Andreas Reisch
Zwitterionic Stealth Dye-Loaded Polymer Nanoparticles for Intracellular Imaging
published pages: 117-125, ISSN: 1944-8244, DOI: 10.1021/acsami.9b15396
ACS Applied Materials & Interfaces 12/1 2020-02-19
2018 Andreas Reisch, Doriane Heimburger, Pauline Ernst, Anne Runser, Pascal Didier, Denis Dujardin, Andrey S. Klymchenko
Protein‐Sized Dye‐Loaded Polymer Nanoparticles for Free Particle Diffusion in Cytosol
published pages: 1805157, ISSN: 1616-301X, DOI: 10.1002/adfm.201805157
Advanced Functional Materials 28/48 2020-02-19
2018 Bohdan Andreiuk, Andreas Reisch, Eduard Bernhardt, Andrey S. Klymchenko
Fighting Aggregation‐Caused Quenching and Leakage of Dyes in Fluorescent Polymer Nanoparticles: Universal Role of Counterion
published pages: 836-846, ISSN: 1861-4728, DOI: 10.1002/asia.201801592
Chemistry – An Asian Journal 14/6 2020-02-19
2019 Marcelina Cardoso Dos Santos, Anne Runser, Hortense Bartenlian, Aline M. Nonat, Loïc J. Charbonnière, Andrey S. Klymchenko, Niko Hildebrandt, Andreas Reisch
Lanthanide-Complex-Loaded Polymer Nanoparticles for Background-Free Single-Particle and Live-Cell Imaging
published pages: 4034-4041, ISSN: 0897-4756, DOI: 10.1021/acs.chemmater.9b00576
Chemistry of Materials 31/11 2020-02-19
2018 Mayeul Collot, Emmanuel Boutant, Maxime Lehmann, Andrey S. Klymchenko
BODIPY with Tuned Amphiphilicity as a Fluorogenic Plasma Membrane Probe
published pages: 192-199, ISSN: 1043-1802, DOI: 10.1021/acs.bioconjchem.8b00828
Bioconjugate Chemistry 30/1 2020-02-19
2019 Vitalii Rosiuk, Anne Runser, Andrey Klymchenko, Andreas Reisch
Controlling Size and Fluorescence of Dye-Loaded Polymer Nanoparticles through Polymer Design
published pages: 7009-7017, ISSN: 0743-7463, DOI: 10.1021/acs.langmuir.9b00721
Langmuir 35/21 2020-02-19
2020 Mayeul Collot, Emmanuel Boutant, Kyong Tkhe Fam, Lydia DANGLOT, Andrey S. Klymchenko
Molecular Tuning of Styryl Dyes Leads to Versatile and Efficient Plasma Membrane Probes for Cell and Tissue Imaging
published pages: , ISSN: 1043-1802, DOI: 10.1021/acs.bioconjchem.0c00023
Bioconjugate Chemistry 2020-02-19
2019 Dmytro I. Danylchuk, Seonah Moon, Ke Xu, Andrey S. Klymchenko
Switchable Solvatochromic Probes for Live-Cell Super-resolution Imaging of Plasma Membrane Organization
published pages: 14920-14924, ISSN: 1433-7851, DOI: 10.1002/anie.201907690
Angewandte Chemie International Edition 58/42 2020-02-19
2019 Kateryna Trofymchuk, Jurga Valanciunaite, Bohdan Andreiuk, Andreas Reisch, Mayeul Collot, Andrey S. Klymchenko
BODIPY-loaded polymer nanoparticles: chemical structure of cargo defines leakage from nanocarrier in living cells
published pages: 5199-5210, ISSN: 2050-7518, DOI: 10.1039/c8tb02781a
Journal of Materials Chemistry B 7/34 2020-02-19
2016 Iuliia A. Karpenko, Yosuke Niko, Viktor P. Yakubovskyi, Andriy O. Gerasov, Dominique Bonnet, Yuriy P. Kovtun, Andrey S. Klymchenko
Push–pull dioxaborine as fluorescent molecular rotor: far-red fluorogenic probe for ligand–receptor interactions
published pages: 3002-3009, ISSN: 2050-7534, DOI: 10.1039/C5TC03411F
J. Mater. Chem. C 4/14 2019-06-06
2017 Fanny Cayre, Simona Mura, Bohdan Andreiuk, Dunja Sobot, Sandrine Gouazou, Didier Desmaële, Andrey S. Klymchenko, Patrick Couvreur
In Vivo FRET Imaging to Predict the Risk Associated with Hepatic Accumulation of Squalene-Based Prodrug Nanoparticles
published pages: 1700830, ISSN: 2192-2640, DOI: 10.1002/adhm.201700830
Advanced Healthcare Materials 2019-06-06
2017 Kateryna Trofymchuk, Andreas Reisch, Pascal Didier, François Fras, Pierre Gilliot, Yves Mely, Andrey S. Klymchenko
Giant light-harvesting nanoantenna for single-molecule detection in ambient light
published pages: 657-663, ISSN: 1749-4885, DOI: 10.1038/s41566-017-0001-7
Nature Photonics 11/10 2019-06-06
2016 Vandamme, Thierry; Klymchenko, Andrey S; Anton, Nicolas; Mercier, Luc; Andreiuk, Bohdan; Goetz, Jacky G; Mély, Yves; Bouchaala, Redouane
Integrity of lipid nanocarriers in bloodstream and tumor quantified by near-infrared ratiometric FRET imaging in living mice
published pages: 57–67, ISSN: 0168-3659, DOI: 10.1016/j.jconrel.2016.06.027
Journal of Controlled Release 236 2019-06-06
2016 Ievgen Shulov, Roman V. Rodik, Youri Arntz, Andreas Reisch, Vitaly I. Kalchenko, Andrey S. Klymchenko
Protein-Sized Bright Fluorogenic Nanoparticles Based on Cross-Linked Calixarene Micelles with Cyanine Corona
published pages: 15884-15888, ISSN: 1433-7851, DOI: 10.1002/anie.201609138
Angewandte Chemie International Edition 55/51 2019-06-06
2017 Redouane Bouchaala, Nicolas Anton, Halina Anton, Thierry Vandamme, Julien Vermot, Djabi Smail, Yves Mély, Andrey S. Klymchenko
Light-triggered release from dye-loaded fluorescent lipid nanocarriers in vitro and in vivo
published pages: 414-421, ISSN: 0927-7765, DOI: 10.1016/j.colsurfb.2017.05.035
Colloids and Surfaces B: Biointerfaces 156 2019-06-06
2016 Yosuke Niko, Pascal Didier, Yves Mely, Gen-ichi Konishi, Andrey S. Klymchenko
Bright and photostable push-pull pyrene dye visualizes lipid order variation between plasma and intracellular membranes
published pages: 18870, ISSN: 2045-2322, DOI: 10.1038/srep18870
Scientific Reports 6/1 2019-06-06
2015 Ievgen Shulov, Sule Oncul, Andreas Reisch, Youri Arntz, Mayeul Collot, Yves Mely, Andrey S. Klymchenko
Fluorinated counterion-enhanced emission of rhodamine aggregates: ultrabright nanoparticles for bioimaging and light-harvesting
published pages: 18198-18210, ISSN: 2040-3364, DOI: 10.1039/C5NR04955E
Nanoscale 7/43 2019-06-06
2017 Klymchenko, Andrey
BrightSens - Ultrabright Turn-on Fluorescent Organic Nanoparticles for Amplified Molecular Sensing in Living Cells - H2020
published pages: 78-80, ISSN: 2398-7073, DOI:
Impact 6/2017 2019-06-06
2016 Luca Petrizza, Mayeul Collot, Ludovic Richert, Yves Mely, Luca Prodi, Andrey S. Klymchenko
Dye-doped silica nanoparticle probes for fluorescence lifetime imaging of reductive environments in living cells
published pages: 104164-104172, ISSN: 2046-2069, DOI: 10.1039/C6RA21427D
RSC Adv. 6/106 2019-06-06
2017 Bohdan Andreiuk, Andreas Reisch, Vasyl G. Pivovarenko, Andrey S. Klymchenko
An aluminium-based fluorinated counterion for enhanced encapsulation and emission of dyes in biodegradable polymer nanoparticles
published pages: 2309-2316, ISSN: 2052-1537, DOI: 10.1039/c7qm00248c
Materials Chemistry Frontiers 1/11 2019-06-06
2017 Andreas Reisch, Kateryna Trofymchuk, Anne Runser, Guillaume Fleith, Michel Rawiso, Andrey S. Klymchenko
Tailoring Fluorescence Brightness and Switching of Nanoparticles through Dye Organization in the Polymer Matrix
published pages: 43030-43042, ISSN: 1944-8244, DOI: 10.1021/acsami.7b12292
ACS Applied Materials & Interfaces 9/49 2019-06-06
2017 Mayeul Collot, Flavien Ponsot, Andrey S. Klymchenko
Ca-NIR: a ratiometric near-infrared calcium probe based on a dihydroxanthene-hemicyanine fluorophore
published pages: 6117-6120, ISSN: 1359-7345, DOI: 10.1039/c7cc02418e
Chemical Communications 53/45 2019-06-06
2017 Bohdan Andreiuk, Andreas Reisch, Marion Lindecker, Gautier Follain, Nadine Peyriéras, Jacky G. Goetz, Andrey S. Klymchenko
Fluorescent Polymer Nanoparticles for Cell Barcoding In Vitro and In Vivo
published pages: 1701582, ISSN: 1613-6810, DOI: 10.1002/smll.201701582
Small 13/38 2019-06-06
2016 Ievgen Shulov, Youri Arntz, Yves Mély, Vasyl G. Pivovarenko, Andrey S. Klymchenko
Non-coordinating anions assemble cyanine amphiphiles into ultra-small fluorescent nanoparticles
published pages: 7962-7965, ISSN: 1359-7345, DOI: 10.1039/C6CC03716J
Chem. Commun. 52/51 2019-06-06
2016 Andreas Reisch, Andrey S. Klymchenko
Fluorescent Polymer Nanoparticles Based on Dyes: Seeking Brighter Tools for Bioimaging
published pages: 1968-1992, ISSN: 1613-6810, DOI: 10.1002/smll.201503396
Small 12/15 2019-06-06
2017 Andrey S. Klymchenko
Solvatochromic and Fluorogenic Dyes as Environment-Sensitive Probes: Design and Biological Applications
published pages: 366-375, ISSN: 0001-4842, DOI: 10.1021/acs.accounts.6b00517
Accounts of Chemical Research 50/2 2019-06-06
2018 Mayeul Collot, Tkhe Kyong Fam, Pichandi Ashokkumar, Orestis Faklaris, Thierry Galli, Lydia Danglot, Andrey S. Klymchenko
Ultrabright and Fluorogenic Probes for Multicolor Imaging and Tracking of Lipid Droplets in Cells and Tissues
published pages: 5401-5411, ISSN: 0002-7863, DOI: 10.1021/jacs.7b12817
Journal of the American Chemical Society 140/16 2019-06-06
2017 Flavien Ponsot, Weida Shen, Pichandi Ashokkumar, Etienne Audinat, Andrey S. Klymchenko, Mayeul Collot
PEGylated Red-Emitting Calcium Probe with Improved Sensing Properties for Neuroscience
published pages: 1706-1712, ISSN: 2379-3694, DOI: 10.1021/acssensors.7b00665
ACS Sensors 2/11 2019-06-06

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