Opendata, web and dolomites

NoCut

Detection of Chromatin Bridges during Cytokinesis

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 NoCut project word cloud

Explore the words cloud of the NoCut project. It provides you a very rough idea of what is the project "NoCut" about.

cells    chromatin    molecular    division    homologs    ultra    disease    separation    genome    combining    cytokinesis    chromosome    localization    mitosis    caused    nocut    decatenation    recognition    site    microscopy    delaying    mechanism    animal    vital    multiple    cell    abscission    duplication    dstorm    generate    human    defects    organism    dna    tumours    super    thereby    dicentric    activation    imaging    function    monitors    cellular    survival    parallel    final    correctly    characterization    unprecedented    condensation    bridges    detected    sensors    completion    mechanisms    acting    proteins    assaying    fine    constrains    signal    significance    made    triggered    binding    putative    integrity    differential    basic    suggests    accomplished    resolution    model    signalling    aurora    bridge    basis    composition    daughter    instability    replication    spanning    components    structural    physical    check    preserving    upstream    yeast    budding   

Project "NoCut" data sheet

The following table provides information about the project.

Coordinator
FUNDACIO CENTRE DE REGULACIO GENOMICA 

Organization address
address: CARRER DOCTOR AIGUADER 88
city: BARCELONA
postcode: 8003
website: www.crg.es

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Spain [ES]
 Project website http://www.crg.eu/en/programmes-groups/coordination-cytokinesis-chromosome-segregation
 Total cost 158˙121 €
 EC max contribution 158˙121 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2015
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-03-01   to  2018-02-28

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    FUNDACIO CENTRE DE REGULACIO GENOMICA ES (BARCELONA) coordinator 158˙121.00

Map

 Project objective

Duplication of the genome and its division into two daughter cells during mitosis is vital for survival of the organism. Cells have multiple mechanisms to ensure that this process is accomplished correctly thereby preserving the integrity of the genome. The final check before cell division is made by the NoCut abscission pathway. In yeast and animal cells, this mechanism monitors completion of chromosome separation, delaying abscission when chromosome bridges spanning the division site are detected. Aurora B is essential for NoCut function, and several of its targets in this pathway have been identified. In budding yeast, NoCut can be triggered by bridges caused by defects in chromosome condensation, decatenation and replication but importantly not by dicentric chromosome bridges. This suggests that structural features of chromatin bridges are essential to generate the NoCut signal. We will investigate the molecular basis of this differential bridge recognition and the signalling pathway acting upstream of Aurora B. We will define the composition of fine and ultra-fine chromatin bridges during cytokinesis in human cells at unprecedented resolution by super-resolution microscopy using dSTORM imaging. In parallel, we will use budding yeast to investigate the role of DNA binding proteins as sensors in the NoCut pathway. We will then establish the significance of these findings in human cells, by assaying the function of putative homologs in NoCut, and their localization in chromatin bridges by dSTORM. By combining approaches in two model systems we will define both the molecular and physical constrains for NoCut activation upstream of the established components of the NoCut pathway. Chromosome instability is associated with many human tumours and in some cases with advanced disease making the detailed characterization of this pathway relevant in our understanding of both basic cellular processes and human disease.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "NOCUT" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "NOCUT" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

CoCoNat (2019)

Coordination in constrained and natural distributed systems

Read More  

DNANanoProbes (2019)

Design of light-harvesting DNA-nanoprobes with ratiometric signal amplification for fluorescence imaging of live cells.

Read More  

TOPOCIRCUS (2019)

Simulations of Topological Phases in Superconducting Circuits

Read More