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CaTSYS SIGNED

Screening, characterisation and engineering of carbohydrate harvesting machineries of microbial ecosystems

Total Cost €

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EC-Contrib. €

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Partnership

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Project "CaTSYS" data sheet

The following table provides information about the project.

Coordinator
INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE TOULOUSE 

Organization address
address: AVENUE DE RANGUEIL 135
city: TOULOUSE
postcode: 31077
website: www.insa-toulouse.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Total cost 173˙076 €
 EC max contribution 173˙076 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2015
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-09-01   to  2018-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE TOULOUSE FR (TOULOUSE) coordinator 173˙076.00

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 Project objective

Mammals rely entirely on their symbiotic microorganisms to digest complex glycans, issued from dietary fibers, yeasts, and the host itself. To that purpose, gut bacteria have evolved sophisticated machineries to face the structural diversity and complexity of carbohydrates, consisting of gene clusters coding for carbohydrate-hydrolysing, -binding and -transporting proteins. Since the last decade, functional metagenomics has proved to be a highly powerful technique to boost the discovery of new protein families involved in these multi-proteic systems from the vast world of uncultured bacteria, which represents the majority of gut microorganisms. By using a multidisciplinary approach, integrating genomic, metagenomic, functional and structural data, CaTSYS will decipher the structure-function relationships of novel key carbohydrate active enzymes and transporters recently discovered by functional exploration of the human and bovine gut microbiomes. Their role in carbohydrate harvesting pathways will be investigated and optimised by combinatorial engineering, thanks to the development of an original high-throughput screening approach, to render heterologous hosts capable of internalising and breaking down a large diversity of complex carbohydrates. Overall, CaTSYS will provide a promising framework to overcome future challenges in biotechnology, for the conversion of carbohydrate polymers into biosourced chemicals and/or high-added value compounds.

 Publications

year authors and title journal last update
List of publications.
2018 Adilya Dagkesamanskaya, Krzysztof Langer, Alexandra S. Tauzin, Catherine Rouzeau, Delphine Lestrade, Gabrielle Potocki-Veronese, Laurent Boitard, Jérôme Bibette, Jean Baudry, Denis Pompon, Véronique Anton-Leberre
Use of photoswitchable fluorescent proteins for droplet-based microfluidic screening
published pages: 59-65, ISSN: 0167-7012, DOI: 10.1016/j.mimet.2018.03.001
Journal of Microbiological Methods 147 2019-06-13

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