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ZF-ILC

Characterization of zebrafish innate lymphoid cells and IL-22

Total Cost €

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EC-Contrib. €

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Partnership

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Project "ZF-ILC" data sheet

The following table provides information about the project.

Coordinator
INSTITUT PASTEUR 

Organization address
address: RUE DU DOCTEUR ROUX 25-28
city: PARIS CEDEX 15
postcode: 75724
website: http://www.pasteur.fr

contact info
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name: n.a.
surname: n.a.
function: n.a.
email: n.a.
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 Coordinator Country France [FR]
 Project website https://research.pasteur.fr/en/project/innate-lymphoid-cells-and-il-22-functional-analysis-in-zebrafish/
 Total cost 173˙076 €
 EC max contribution 173˙076 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2015
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-09-01   to  2019-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    INSTITUT PASTEUR FR (PARIS CEDEX 15) coordinator 173˙076.00

Map

 Project objective

The immune system can be subdivided into two main arms: the innate and the adaptive. Recently, it was identified a new family of cells belonging to the innate system but that phenotypically and functionally resemble the specific subtypes of adaptive T lymphocytes. In mice and in humans, these innate lymphoid cells (ILCs) play critical roles in host defense, in shaping the microbiota and in tissue repair at mucosal tissues via secreting cytokines such as IL-22. The innate system is more ancient than the adaptive, however, the existence and diversity of ILCs in organisms other than mammals remains unknown. Also unknown is how ILCs interact with other cells within tissues. This project will address the existence and role of the different subsets of ILCs and the conservation of IL-22 function in a distant relative, the zebrafish, a genetically tractable vertebrate with remarkable optical accessibility. To identify ILCs, cell populations likely enriched in these cells will be purified from mucosal sites such as the intestine and the gills by flow cytometry, guided by morphology and expression of reporter transgenes for immune cell types. Their transcriptome profile will be assessed in bulk and at the single-cell level adapting the microfluidic-based Biomark system to the zebrafish. Also, to know if cytokines produced by T cells can also be produced by non-adaptive lymphocytes – i.e., ILCs, as described in mouse, immune responses of zebrafish mutants lacking adaptive lymphocytes or all types of lymphocytes will be compared to that of wild-type fish. To study IL-22 function, mutants for this cytokine and its receptor will be generated. Together with a transgenic il22 reporter line allowing the identification of IL-22-producing cells, they will be challenged with pathogens or inflammatory stimuli, and studied by various approaches including in vivo imaging. This work will allow a detailed in vivo study of the ontogeny, role and behavior of ILCs and IL-22- producing cells.

 Publications

year authors and title journal last update
List of publications.
2019 Maximo Coronado, Camila J. Solis, Pedro P. Hernandez, Carmen G. Feijóo
Soybean Meal-Induced Intestinal Inflammation in Zebrafish Is T Cell-Dependent and Has a Th17 Cytokine Profile
published pages: , ISSN: 1664-3224, DOI: 10.3389/fimmu.2019.00610
Frontiers in Immunology 10 2020-01-29
2018 Pedro P. Hernández, Paulina M. Strzelecka, Emmanouil I. Athanasiadis, Dominic Hall, Ana F. Robalo, Catherine M. Collins, Pierre Boudinot, Jean-Pierre Levraud, Ana Cvejic
Single-cell transcriptional analysis reveals ILC-like cells in zebrafish
published pages: eaau5265, ISSN: 2470-9468, DOI: 10.1126/sciimmunol.aau5265
Science Immunology 3/29 2020-01-29

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