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CondStruct SIGNED

Structural basis for the coordination of chromosome architecture by condensin complexes

Total Cost €

0

EC-Contrib. €

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Partnership

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 CondStruct project word cloud

Explore the words cloud of the CondStruct project. It provides you a very rough idea of what is the project "CondStruct" about.

chromatin    biological    machinery    rearrangements    partitioning    microscopy    roles    spectrometry    gained    ring    confer    topology    disease    molecular    biology    mechanistic    architecture    atpase    conformational    gene    engages    mass    shaped    core    technologies    dependent    model    dimensional    fluorescence    dynamic    genomic    cellular    structures    course    linking    implications    reveal    reconstitution    expression    networks    near    protein    shape    encircles    undergo    linkages    mediated    resolution    plays    entire    dramatic    genomes    single    organisation    division    cycle    activates    despite    integrative    action    atomic    linear    structural    differentiation    ray    regulation    dna    functions    chromosomes    determines    combine    cross    function    molecule    intend    comprehension    interaction    experiments    genome    chromosome    cell    integrity    mechanisms    complementary    insights    duplication    fundamental    biochemical    unravel    fibres    elusive    condensin    electron    subunit    anticipate    helices    ranging    invaluable    crystallography    first   

Project "CondStruct" data sheet

The following table provides information about the project.

Coordinator
EUROPEAN MOLECULAR BIOLOGY LABORATORY 

Organization address
address: Meyerhofstrasse 1
city: HEIDELBERG
postcode: 69117
website: http://www.embl.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Project website http://www.haering.embl.de
 Total cost 1˙982˙479 €
 EC max contribution 1˙982˙479 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2015-CoG
 Funding Scheme ERC-COG
 Starting year 2016
 Duration (year-month-day) from 2016-07-01   to  2021-06-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    EUROPEAN MOLECULAR BIOLOGY LABORATORY DE (HEIDELBERG) coordinator 1˙982˙479.00

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 Project objective

Chromosomes undergo dramatic changes in their three-dimensional organisation during all aspects of genome function, ranging from the regulation of gene expression during cellular differentiation to chromosome duplication and partitioning over the course of a cell division cycle. The multi-subunit condensin protein complex plays major roles for these changes in DNA topology. Despite its fundamental importance, the mechanisms of condensin’s action are not understood.

Here, I propose a comprehensive research program that aims to reveal the elusive mechanisms behind the functions of the condensin complex. We intend to unravel how the condensin complex engages DNA, how this interaction activates large-scale ATPase-dependent conformational rearrangements within the complex, and how condensin eventually encircles chromatin fibres within its ring-shaped architecture. Insights from these mechanistic studies will be invaluable for understanding how networks of condensin-mediated linkages can shape linear DNA helices into higher-order chromosome structures. To achieve this ambitious and timely goal, we will combine an integrative structural biology approach with biochemical and cell biological methods. By applying complementary technologies, including X-ray protein crystallography, electron microscopy, cross-linking mass spectrometry, single molecule fluorescence microscopy and reconstitution experiments, we anticipate to build the first model of the entire condensin complex at near-atomic resolution and explain how dynamic conformational changes confer function.

The insights gained from this research program will provide an in-depth mechanistic comprehension of the core molecular machinery that determines the architecture of our genomes and will have major implications for understanding how genomic integrity is affected in various disease conditions.

 Publications

year authors and title journal last update
List of publications.
2019 Markus Hassler, Indra A. Shaltiel, Marc Kschonsak, Bernd Simon, Fabian Merkel, Lena Thärichen, Henry J. Bailey, Jakub Macošek, Sol Bravo, Jutta Metz, Janosch Hennig, Christian H. Haering
Structural Basis of an Asymmetric Condensin ATPase Cycle
published pages: 1175-1188.e9, ISSN: 1097-2765, DOI: 10.1016/j.molcel.2019.03.037
Molecular Cell 74/6 2019-09-02
2017 Jorine M Eeftens, Shveta Bisht, Jacob Kerssemakers, Marc Kschonsak, Christian H Haering, Cees Dekker
Real‐time detection of condensin‐driven DNA compaction reveals a multistep binding mechanism
published pages: 3448-3457, ISSN: 0261-4189, DOI: 10.15252/embj.201797596
The EMBO Journal 36/23 2019-06-18
2017 Tsuyoshi Terakawa, Shveta Bisht, Jorine M. Eeftens, Cees Dekker, Christian H. Haering, Eric C. Greene
The condensin complex is a mechanochemical motor that translocates along DNA
published pages: 672-676, ISSN: 0036-8075, DOI: 10.1126/science.aan6516
Science 358/6363 2019-06-18
2017 Marc Kschonsak, Fabian Merkel, Shveta Bisht, Jutta Metz, Vladimir Rybin, Markus Hassler, Christian H. Haering
Structural Basis for a Safety-Belt Mechanism That Anchors Condensin to Chromosomes
published pages: 588-600.e24, ISSN: 0092-8674, DOI: 10.1016/j.cell.2017.09.008
Cell 171/3 2019-06-18
2018 Mahipal Ganji, Indra A. Shaltiel, Shveta Bisht, Eugene Kim, Ana Kalichava, Christian H. Haering, Cees Dekker
Real-time imaging of DNA loop extrusion by condensin
published pages: 102-105, ISSN: 0036-8075, DOI: 10.1126/science.aar7831
Science 360/6384 2019-04-20

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