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CondStruct SIGNED

Structural basis for the coordination of chromosome architecture by condensin complexes

Total Cost €

0

EC-Contrib. €

0

Partnership

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 CondStruct project word cloud

Explore the words cloud of the CondStruct project. It provides you a very rough idea of what is the project "CondStruct" about.

disease    fibres    reconstitution    biological    mechanistic    microscopy    comprehension    cell    condensin    genomic    atpase    crystallography    differentiation    action    plays    shape    conformational    partitioning    linking    biology    interaction    complementary    expression    dependent    combine    entire    structural    chromosome    rearrangements    cross    gene    reveal    first    undergo    invaluable    roles    spectrometry    linkages    architecture    encircles    linear    organisation    anticipate    shaped    determines    technologies    biochemical    division    confer    atomic    resolution    ring    insights    fluorescence    subunit    mass    course    intend    mediated    structures    ray    function    networks    cycle    fundamental    dimensional    chromatin    single    experiments    protein    helices    despite    functions    ranging    near    integrative    genome    mechanisms    dramatic    topology    implications    molecular    cellular    core    chromosomes    genomes    duplication    electron    gained    integrity    model    elusive    unravel    regulation    molecule    dna    engages    machinery    dynamic    activates   

Project "CondStruct" data sheet

The following table provides information about the project.

Coordinator
EUROPEAN MOLECULAR BIOLOGY LABORATORY 

Organization address
address: Meyerhofstrasse 1
city: HEIDELBERG
postcode: 69117
website: http://www.embl.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Project website http://www.haering.embl.de
 Total cost 1˙982˙479 €
 EC max contribution 1˙982˙479 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2015-CoG
 Funding Scheme ERC-COG
 Starting year 2016
 Duration (year-month-day) from 2016-07-01   to  2021-06-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    EUROPEAN MOLECULAR BIOLOGY LABORATORY DE (HEIDELBERG) coordinator 1˙982˙479.00

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 Project objective

Chromosomes undergo dramatic changes in their three-dimensional organisation during all aspects of genome function, ranging from the regulation of gene expression during cellular differentiation to chromosome duplication and partitioning over the course of a cell division cycle. The multi-subunit condensin protein complex plays major roles for these changes in DNA topology. Despite its fundamental importance, the mechanisms of condensin’s action are not understood.

Here, I propose a comprehensive research program that aims to reveal the elusive mechanisms behind the functions of the condensin complex. We intend to unravel how the condensin complex engages DNA, how this interaction activates large-scale ATPase-dependent conformational rearrangements within the complex, and how condensin eventually encircles chromatin fibres within its ring-shaped architecture. Insights from these mechanistic studies will be invaluable for understanding how networks of condensin-mediated linkages can shape linear DNA helices into higher-order chromosome structures. To achieve this ambitious and timely goal, we will combine an integrative structural biology approach with biochemical and cell biological methods. By applying complementary technologies, including X-ray protein crystallography, electron microscopy, cross-linking mass spectrometry, single molecule fluorescence microscopy and reconstitution experiments, we anticipate to build the first model of the entire condensin complex at near-atomic resolution and explain how dynamic conformational changes confer function.

The insights gained from this research program will provide an in-depth mechanistic comprehension of the core molecular machinery that determines the architecture of our genomes and will have major implications for understanding how genomic integrity is affected in various disease conditions.

 Publications

year authors and title journal last update
List of publications.
2019 Markus Hassler, Indra A. Shaltiel, Marc Kschonsak, Bernd Simon, Fabian Merkel, Lena Thärichen, Henry J. Bailey, Jakub Macošek, Sol Bravo, Jutta Metz, Janosch Hennig, Christian H. Haering
Structural Basis of an Asymmetric Condensin ATPase Cycle
published pages: 1175-1188.e9, ISSN: 1097-2765, DOI: 10.1016/j.molcel.2019.03.037
Molecular Cell 74/6 2019-09-02
2017 Jorine M Eeftens, Shveta Bisht, Jacob Kerssemakers, Marc Kschonsak, Christian H Haering, Cees Dekker
Real‐time detection of condensin‐driven DNA compaction reveals a multistep binding mechanism
published pages: 3448-3457, ISSN: 0261-4189, DOI: 10.15252/embj.201797596
The EMBO Journal 36/23 2019-06-18
2017 Tsuyoshi Terakawa, Shveta Bisht, Jorine M. Eeftens, Cees Dekker, Christian H. Haering, Eric C. Greene
The condensin complex is a mechanochemical motor that translocates along DNA
published pages: 672-676, ISSN: 0036-8075, DOI: 10.1126/science.aan6516
Science 358/6363 2019-06-18
2017 Marc Kschonsak, Fabian Merkel, Shveta Bisht, Jutta Metz, Vladimir Rybin, Markus Hassler, Christian H. Haering
Structural Basis for a Safety-Belt Mechanism That Anchors Condensin to Chromosomes
published pages: 588-600.e24, ISSN: 0092-8674, DOI: 10.1016/j.cell.2017.09.008
Cell 171/3 2019-06-18
2018 Mahipal Ganji, Indra A. Shaltiel, Shveta Bisht, Eugene Kim, Ana Kalichava, Christian H. Haering, Cees Dekker
Real-time imaging of DNA loop extrusion by condensin
published pages: 102-105, ISSN: 0036-8075, DOI: 10.1126/science.aar7831
Science 360/6384 2019-04-20

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