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MERA SIGNED

Mechanism of Enzyme Rhodopsin Activation

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
results

MERA project word cloud

Explore the words cloud of the MERA project. It provides you a very rough idea of what is the project "MERA" about.

neurons fungus mechanism optogenetic though guanylyl obtain raman family time intensities totally phosphodiesterase urgently ultimate inactivation revolutionized regulation channel flashes conversion dimensional aided emersonii perspectives solution light final desirable subdivided elegant variety first biophysical ray neuroscience cyclase epr model messenger characterization rhpde neuronal activation offers laboratory rhodopsin camp broad coexpression optogenetics ensemble ftir photoreceptors accordingly spectroscopy generation decade dynamics blastocladiella hypopolarizer uv lines class enzyme computer activate rhgc fourth gated moderate uncharacterized resolved mera photoreceptor crystallography cgmp crystals awaits vis understand discovered technologies cell sensory outcome ion channelrhodopsin engineering cells

Project "MERA" data sheet

The following table provides information about the project.

Coordinator	HUMBOLDT-UNIVERSITAET ZU BERLIN Organization address address: UNTER DEN LINDEN 6 city: BERLIN postcode: 10117 website: www.hu-berlin.de contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Germany [DE]
Total cost	2˙398˙750 €
EC max contribution	2˙398˙750 € (100%)
Programme	1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
Code Call	ERC-2015-AdG
Funding Scheme	ERC-ADG
Starting year	2016
Duration (year-month-day)	from 2016-10-01 to 2021-09-30

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	HUMBOLDT-UNIVERSITAET ZU BERLIN HUMBOLDT-UNIVERSITAET ZU BERLIN Organization address address: UNTER DEN LINDEN 6 city: BERLIN postcode: 10117 website: www.hu-berlin.de contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	DE (BERLIN)	coordinator	2˙398˙750.00

Map

Project objective

'Channelrhodopsin, which was discovered and described as a light-gated ion channel in my laboratory, has revolutionized the field of neuroscience over the past decade by enabling researchers to specifically activate selected neurons in a large ensemble of neuronal cells with short light flashes, a technology we now call 'Optogenetics.' However, though highly desirable, the inactivation of specific cells using moderate or low light intensities is not yet possible. The recently discovered rhodopsin-guanylyl-cyclase (RhGC) of the fungus Blastocladiella emersonii offers an elegant solution to this problem. Moreover, RhGC is a totally novel and uncharacterized sensory photoreceptor, and the first member of an enzyme rhodopsin family that urgently awaits in-depth characterization. Accordingly, the goal of the “mechanism of enzyme rhodopsin activation” (MERA) proposal is to obtain a comprehensive understanding of this novel photoreceptor, and to determine its functionality for broad application in optogenetics and other research fields. The MERA project is subdivided into four objectives. The first objective is the characterization and engineering of RhGC in cell lines and neurons as well as coexpression of RhGC with a cGMP-gated K channel to develop a 'Light-Hypopolarizer' for cell inactivation. The second objective is to understand the dynamics of RhGC using a variety of biophysical technologies including time resolved UV-vis, FTIR, and Raman and EPR spectroscopy. A third objective is the generation of crystals for X-ray crystallography and the development of a three dimensional RhGC model. The fourth and final objective is the computer-aided conversion of RhGC into a rhodopsin-phosphodiesterase (RhPDE) for down-regulation of the second messenger cGMP and/or cAMP using light. The ultimate outcome will be a detailed understanding of a novel class of sensory photoreceptors with new perspectives for broad optogenetic applications.'

Publications

List of publications.
year	authors and title	journal	last update
2018	Ulrike Scheib, Matthias Broser, Oana M. Constantin, Shang Yang, Shiqiang Gao, Shatanik Mukherjee, Katja Stehfest, Georg Nagel, Christine E. Gee, Peter Hegemann Rhodopsin-cyclases for photocontrol of cGMP/cAMP and 2.3â€‰Ã… structure of the adenylyl cyclase domain published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-018-04428-w	Nature Communications 9/1	2019-06-13
2017	Alfons Penzkofer, Ulrike Scheib, Katja Stehfest, Peter Hegemann Absorption and Emission Spectroscopic Investigation of Thermal Dynamics and Photo-Dynamics of the Rhodopsin Domain of the Rhodopsin-Guanylyl Cyclase from the Nematophagous Fungus Catenaria anguillulae published pages: 2099, ISSN: 1422-0067, DOI: 10.3390/ijms18102099	International Journal of Molecular Sciences 18/10	2019-06-13
2019	Scheib, U. Biochemische und biophysikalische Charakterisierung von Rhodopsin-Guanylylzyklasen. published pages: , ISSN: , DOI:		2019-06-06
2019	Shatanik Mukherjee, Peter Hegemann, Matthias Broser Enzymerhodopsins: novel photoregulated catalysts for optogenetics published pages: 118-126, ISSN: 0959-440X, DOI: 10.1016/j.sbi.2019.02.003	Current Opinion in Structural Biology 57	2019-06-06

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