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Dissecting Rho GTPase signalling networks through acute perturbation techniques

Total Cost €


EC-Contrib. €






 RhoSNATCH project word cloud

Explore the words cloud of the RhoSNATCH project. It provides you a very rough idea of what is the project "RhoSNATCH" about.

patterns    networks    image    living    insights    scales    cdc42    rhoa    power    spatiotemporal    chemical    perturbing    spatiotemporally    vision    rho    tunes    maintains    subminute    functional    rac1    multiple    subset    zones    genome    gtpases    edge    newly    cytoskeletal    dissect    modules    tune    resolution    questions    data    endogenous    regulators    experiments    micrometer    timescales    signaling    single    space    precise    upstream    induce    circuitry    rhosnatch    editing    recording    functions    first    downstream    systematically    gtpase    perturbed    fibroblast    give    live    acutely    fibroblasts    positions    dynamics    extension    outputs    activation    perturb    combination    fluctuate    models    dimerizing    length    recent    computer    cell    migration    led    assisted    reversible    unprecedented    discovered    complexity    imaging    output    crosstalk    regulate    recorded    multiplexing    molecular    motile    technologies    fine    microfluidic    complexes    time    cells    identification    biologically   

Project "RhoSNATCH" data sheet

The following table provides information about the project.


Organization address
city: BERN
postcode: 3012

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Project website
 Total cost 187˙419 €
 EC max contribution 187˙419 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-04-01   to  2019-03-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITAET BERN CH (BERN) coordinator 187˙419.00


 Project objective

Recent advances in technologies to measure Rho GTPase signaling with unprecedented spatiotemporal resolution in single living cells have led to novel questions. Important new insights are that Rho GTPase signaling involves the formation of signaling complexes that fluctuate on subminute time and micrometer length scales, and that complex signaling networks involving multiple Rho GTPases fine tune the leading edge dynamics that power fibroblast migration. Novel methodologies are required to dissect this newly discovered signaling complexity. RhoSNATCH aims to characterize these signaling networks by acutely perturbing and recording Rho GTPase signaling at biologically relevant timescales in live-cell imaging experiments of cell migration. I will identify the crosstalk between Rac1/RhoA/Cdc42 that positions and maintains specific Rho GTPase activity zones in time and space and identify dedicated functional signaling modules that fine tune specific cytoskeletal output at the leading edge of motile fibroblasts. Microfluidic technology will be used to induce precise fibroblast leading edge signaling states. Genome editing in combination with a reversible chemical dimerizing system will be used to acutely perturb endogenous RhoA,Cdc42 and Rac1 signaling, as well as a subset of their upstream regulators that regulate leading edge dynamics. Rho GTPase activation patterns and downstream cytoskeletal outputs will be systematically recorded in the different perturbed states. Computer-vision assisted image analysis will allow the multiplexing of multiple data sets to produce one of the first integrated models of the molecular circuitry that fine-tunes Rho GTPase signaling and cytoskeletal dynamics during leading edge extension. This novel, integrated approach will allow for the first time the identification of different spatiotemporally organised Rho GTPase signaling modules, and give relevant insight into the crosstalk between and functions of Rho GTPases in time and space.

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The information about "RHOSNATCH" are provided by the European Opendata Portal: CORDIS opendata.

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