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EpiScope SIGNED

Epigenomics and chromosome architecture one cell at a time

Total Cost €

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EC-Contrib. €

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Partnership

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 EpiScope project word cloud

Explore the words cloud of the EpiScope project. It provides you a very rough idea of what is the project "EpiScope" about.

tissue    genome    architecture    interplay    molecular    molecule    unveil    thousands    structure    eukaryotes    combinations    100    maintenance    scales    multiple    transcriptional    limited    performance    memory    localization    structures    dna    sub    functional    hi    transcription    conformations    epigenomic    microfluidics    microscopies    variations    nucleosomes    marks    nuclear    epigenetic    genomic    landscape    map    relationships    loci    light    groundbreaking    technologies    types    genes    functions    territories    preserving    episcope    megabase    single    mechanisms    until    distance    packing    organization    lack    topologically    full    cellular    nanoscale    intermediate    correlated    3d    associating    specificity    critically    chromatin    linked    differentiation    shed    documented    efficient    chromosomes    tads    context    throughput    implicated    length    domains    patterns    content    establishment    bp    identity    toolbox    labeling    function    cell    responsible    actors    pairwise    correlate    nucleus    chromosome   

Project "EpiScope" data sheet

The following table provides information about the project.

Coordinator
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS 

Organization address
address: RUE MICHEL ANGE 3
city: PARIS
postcode: 75794
website: www.cnrs.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Total cost 1˙999˙780 €
 EC max contribution 1˙999˙780 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-COG
 Funding Scheme ERC-COG
 Starting year 2017
 Duration (year-month-day) from 2017-09-01   to  2022-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS FR (PARIS) coordinator 1˙999˙780.00

Map

 Project objective

In Eukaryotes, cellular identity and tissue-specific functions are linked to the epigenetic landscape and the multi-scale architecture of the genome. The packing of DNA into nucleosomes at the ~100 bp scale and the organization of whole chromosomes into functional territories within the nucleus are well documented. At an intermediate scale, chromosomes are organised in megabase to sub-megabase structures called Topologically Associating Domains (TADs). Critically, TADs are highly correlated to patterns of epigenetic marks determining the transcriptional state of the genes they encompass. Until now, the lack of efficient technologies to map chromosome architecture and epigenetic marks at the single-cell level have limited our understanding of the molecular actors and mechanisms implicated in the establishment and maintenance of the multi-scale architecture of chromosomes and epigenetic states, and the interplay between this architecture and other nuclear functions such as transcription. The overall aim of EpiScope is to unveil the functional, multi-scale, 3D architecture of chromatin at the single-cell level while preserving cellular context, with a toolbox of groundbreaking high-performance microscopies (Hi-M). Hi-M will use unique combinations of multi-focus and single-molecule localization microscopies with novel DNA labeling methods and microfluidics. Hi-M will enable the study of structure-function relationships within TADs of different chromatin types and correlate single-cell variations in epigenomic patterns to 3D conformations with genomic specificity and at the nanoscale. Finally, Hi-M will be used to develop a novel high-throughput, high-content method to unveil the full pairwise distance distribution between thousands of genomic loci at the single cell level and at multiple length-scales. Our findings and technologies will shed new light into the mechanisms responsible for cellular memory, identity and differentiation.

 Publications

year authors and title journal last update
List of publications.
2019 Andrés M. Cardozo Gizzi, Diego I. Cattoni, Jean-Bernard Fiche, Sergio M. Espinola, Julian Gurgo, Olivier Messina, Christophe Houbron, Yuki Ogiyama, Giorgio L. Papadopoulos, Giacomo Cavalli, Mounia Lagha, Marcelo Nollmann
Microscopy-Based Chromosome Conformation Capture Enables Simultaneous Visualization of Genome Organization and Transcription in Intact Organisms
published pages: 212-222.e5, ISSN: 1097-2765, DOI: 10.1016/j.molcel.2019.01.011
Molecular Cell 74/1 2019-05-22
2018 Quentin Szabo, Daniel Jost, Jia-Ming Chang, Diego I. Cattoni, Giorgio L. Papadopoulos, Boyan Bonev, Tom Sexton, Julian Gurgo, Caroline Jacquier, Marcelo Nollmann, Frédéric Bantignies, Giacomo Cavalli
TADs are 3D structural units of higher-order chromosome organization in Drosophila
published pages: eaar8082, ISSN: 2375-2548, DOI: 10.1126/sciadv.aar8082
Science Advances 4/2 2019-05-22

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