EU H2020 Project "EPISCOPE (Epigenomics and chromosome architecture one cell at a time)": description, partecipants, costs and EC-fundings

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EpiScope project word cloud

Explore the words cloud of the EpiScope project. It provides you a very rough idea of what is the project "EpiScope" about.

3d cellular scales groundbreaking establishment combinations molecular bp sub conformations landscape organization single map chromosomes domains until genes full identity mechanisms content context limited types lack packing technologies light localization microscopies eukaryotes differentiation length interplay microfluidics correlated cell critically territories distance linked genomic memory tads transcription nucleosomes thousands maintenance structure toolbox pairwise structures chromosome functional nanoscale marks megabase intermediate dna patterns nucleus nuclear functions loci responsible associating documented specificity hi function unveil episcope 100 labeling epigenetic implicated tissue architecture preserving molecule chromatin efficient performance epigenomic actors correlate multiple genome throughput shed topologically variations transcriptional relationships

Project "EpiScope" data sheet

The following table provides information about the project.

Coordinator	CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS Organization address address: RUE MICHEL ANGE 3 city: PARIS postcode: 75794 website: www.cnrs.fr contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	France [FR]
Total cost	1˙999˙780 €
EC max contribution	1˙999˙780 € (100%)
Programme	1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
Code Call	ERC-2016-COG
Funding Scheme	ERC-COG
Starting year	2017
Duration (year-month-day)	from 2017-09-01 to 2022-08-31

#	participants	country	role	EC contrib. [€]
1	CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS	FR (PARIS)	coordinator	1˙999˙780.00

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS

FR (PARIS)

coordinator

1˙999˙780.00

Project objective

In Eukaryotes, cellular identity and tissue-specific functions are linked to the epigenetic landscape and the multi-scale architecture of the genome. The packing of DNA into nucleosomes at the ~100 bp scale and the organization of whole chromosomes into functional territories within the nucleus are well documented. At an intermediate scale, chromosomes are organised in megabase to sub-megabase structures called Topologically Associating Domains (TADs). Critically, TADs are highly correlated to patterns of epigenetic marks determining the transcriptional state of the genes they encompass. Until now, the lack of efficient technologies to map chromosome architecture and epigenetic marks at the single-cell level have limited our understanding of the molecular actors and mechanisms implicated in the establishment and maintenance of the multi-scale architecture of chromosomes and epigenetic states, and the interplay between this architecture and other nuclear functions such as transcription. The overall aim of EpiScope is to unveil the functional, multi-scale, 3D architecture of chromatin at the single-cell level while preserving cellular context, with a toolbox of groundbreaking high-performance microscopies (Hi-M). Hi-M will use unique combinations of multi-focus and single-molecule localization microscopies with novel DNA labeling methods and microfluidics. Hi-M will enable the study of structure-function relationships within TADs of different chromatin types and correlate single-cell variations in epigenomic patterns to 3D conformations with genomic specificity and at the nanoscale. Finally, Hi-M will be used to develop a novel high-throughput, high-content method to unveil the full pairwise distance distribution between thousands of genomic loci at the single cell level and at multiple length-scales. Our findings and technologies will shed new light into the mechanisms responsible for cellular memory, identity and differentiation.

Publications

List of publications.
year	authors and title	journal	last update
2019	AndrÃ©s M. Cardozo Gizzi, Diego I. Cattoni, Jean-Bernard Fiche, Sergio M. Espinola, Julian Gurgo, Olivier Messina, Christophe Houbron, Yuki Ogiyama, Giorgio L. Papadopoulos, Giacomo Cavalli, Mounia Lagha, Marcelo Nollmann Microscopy-Based Chromosome Conformation Capture Enables Simultaneous Visualization of Genome Organization and Transcription in Intact Organisms published pages: 212-222.e5, ISSN: 1097-2765, DOI: 10.1016/j.molcel.2019.01.011	Molecular Cell 74/1	2019-05-22
2018	Quentin Szabo, Daniel Jost, Jia-Ming Chang, Diego I. Cattoni, Giorgio L. Papadopoulos, Boyan Bonev, Tom Sexton, Julian Gurgo, Caroline Jacquier, Marcelo Nollmann, FrÃ©dÃ©ric Bantignies, Giacomo Cavalli TADs are 3D structural units of higher-order chromosome organization in Drosophila published pages: eaar8082, ISSN: 2375-2548, DOI: 10.1126/sciadv.aar8082	Science Advances 4/2	2019-05-22

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