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EV-LNC

Extracellular vesicle-mediated delivery of long non-coding RNA: Implications for vascular repair and regeneration

Total Cost €

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EC-Contrib. €

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Partnership

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 EV-LNC project word cloud

Explore the words cloud of the EV-LNC project. It provides you a very rough idea of what is the project "EV-LNC" about.

bioinformatics    macromolecules    diseases    extracellular    respectively    fluorescent    cre    vitro    cytometry    differentiation    intercellular    demonstrated    presume    vesicles    background    comprise    drastically    model    disease    seq    flow    molecules    functions    pioneer    colour    crosstalk    rna    cardiovascular    cutting    proliferation    edge    debris    repair    brand    changed    switch    significantly    mechanisms    implications    transfer    muscle    function    molecular    cargoes    sorted    analysed    signalling    fluorescence    therapy    integration    human    initial    ev    vectors    biology    guide    cell    elucidate    vesicle    data    mediators    communication    vascular    relevance    pulmonary    lncrna    kinds    changing    evs    interpret    lentiviral    regeneration    mechanistic    significance    gapmers    injury    cells    hypertension    rnas    besides    determinant    functional    lncrnas    mediated    endothelial    enriched    sirnas    smooth    recombination    methodology    regulatory    imaging    coding    offers    gain    triggered    driving    arterial    loxp    proteomics    paracrine   

Project "EV-LNC" data sheet

The following table provides information about the project.

Coordinator
THE UNIVERSITY OF EDINBURGH 

Organization address
address: OLD COLLEGE, SOUTH BRIDGE
city: EDINBURGH
postcode: EH8 9YL
website: www.ed.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Project website https://ec.europa.eu/research/participants/portal/desktop/en/projects/details.html
 Total cost 195˙454 €
 EC max contribution 195˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-08-01   to  2019-07-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE UNIVERSITY OF EDINBURGH UK (EDINBURGH) coordinator 195˙454.00

Map

 Project objective

Background: The concept of extracellular vesicles (EVs) has drastically changed from the initial non-functional debris to the current of key mediators of paracrine signalling. The cargoes of EVs comprise all kinds of macromolecules, and recent evidence has demonstrated the presence of long non-coding RNAs (lncRNAs) in such vesicles. These RNA molecules have numerous potential regulatory functions and results obtained so far guide us to presume a determinant role in vascular cell differentiation, proliferation and repair. Besides, the increasing data emerging in the field are significantly changing the way in which we interpret molecular mechanisms driving cardiovascular diseases and offers a brand new set of molecular targets for therapy. For all these reasons, study of lncRNAs in vascular biology and disease is state-of-the-art. Objectives: The main aim of this project is to study extracellular vesicle mediated cell-to-cell communication between human smooth muscle cells and endothelial cells, evaluate its relevance in vascular injury in an in vitro model of pulmonary arterial hypertension, and determine the significance of long non-coding RNA in this crosstalk. Methodology: For imaging EV transfer among vascular cells, we will use a pioneer approach based on Cre-loxP recombination which results in a fluorescent colour switch of cells upon EV uptake. Cells will then be sorted according to fluorescence by flow cytometry and analysed by cutting-edge proteomics and bioinformatics, in order to elucidate intercellular signalling triggered by EV transfer. The lncRNAs present in EVs will be analysed by RNA-Seq. Mechanistic insight of enriched lncRNAs in EVs will be evaluated using gain- and loss-of function approaches in vascular cells using lentiviral vectors and GapmeRs/siRNAs, respectively. Integration of all these analyses will provide key information to define implications of EV-mediated delivery of lncRNA for vascular repair and regeneration.

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