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EV-LNC

Extracellular vesicle-mediated delivery of long non-coding RNA: Implications for vascular repair and regeneration

Total Cost €

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EC-Contrib. €

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Partnership

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 EV-LNC project word cloud

Explore the words cloud of the EV-LNC project. It provides you a very rough idea of what is the project "EV-LNC" about.

smooth    human    functions    switch    cytometry    imaging    loxp    cell    significantly    function    comprise    fluorescence    mediated    pioneer    drastically    rnas    debris    proliferation    intercellular    initial    flow    macromolecules    background    model    mediators    differentiation    respectively    coding    brand    gain    interpret    endothelial    paracrine    changed    repair    mechanisms    seq    molecules    transfer    edge    colour    vitro    integration    demonstrated    relevance    rna    cardiovascular    muscle    guide    extracellular    driving    disease    significance    cre    cargoes    presume    regeneration    besides    functional    vesicles    injury    signalling    sirnas    evs    sorted    determinant    cells    hypertension    fluorescent    elucidate    analysed    changing    arterial    implications    communication    lncrnas    lncrna    data    cutting    proteomics    ev    gapmers    pulmonary    offers    vascular    molecular    triggered    biology    regulatory    diseases    crosstalk    enriched    vectors    lentiviral    mechanistic    kinds    vesicle    recombination    therapy    methodology    bioinformatics   

Project "EV-LNC" data sheet

The following table provides information about the project.

Coordinator
THE UNIVERSITY OF EDINBURGH 

Organization address
address: OLD COLLEGE, SOUTH BRIDGE
city: EDINBURGH
postcode: EH8 9YL
website: www.ed.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Project website https://ec.europa.eu/research/participants/portal/desktop/en/projects/details.html
 Total cost 195˙454 €
 EC max contribution 195˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-08-01   to  2019-07-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE UNIVERSITY OF EDINBURGH UK (EDINBURGH) coordinator 195˙454.00

Map

 Project objective

Background: The concept of extracellular vesicles (EVs) has drastically changed from the initial non-functional debris to the current of key mediators of paracrine signalling. The cargoes of EVs comprise all kinds of macromolecules, and recent evidence has demonstrated the presence of long non-coding RNAs (lncRNAs) in such vesicles. These RNA molecules have numerous potential regulatory functions and results obtained so far guide us to presume a determinant role in vascular cell differentiation, proliferation and repair. Besides, the increasing data emerging in the field are significantly changing the way in which we interpret molecular mechanisms driving cardiovascular diseases and offers a brand new set of molecular targets for therapy. For all these reasons, study of lncRNAs in vascular biology and disease is state-of-the-art. Objectives: The main aim of this project is to study extracellular vesicle mediated cell-to-cell communication between human smooth muscle cells and endothelial cells, evaluate its relevance in vascular injury in an in vitro model of pulmonary arterial hypertension, and determine the significance of long non-coding RNA in this crosstalk. Methodology: For imaging EV transfer among vascular cells, we will use a pioneer approach based on Cre-loxP recombination which results in a fluorescent colour switch of cells upon EV uptake. Cells will then be sorted according to fluorescence by flow cytometry and analysed by cutting-edge proteomics and bioinformatics, in order to elucidate intercellular signalling triggered by EV transfer. The lncRNAs present in EVs will be analysed by RNA-Seq. Mechanistic insight of enriched lncRNAs in EVs will be evaluated using gain- and loss-of function approaches in vascular cells using lentiviral vectors and GapmeRs/siRNAs, respectively. Integration of all these analyses will provide key information to define implications of EV-mediated delivery of lncRNA for vascular repair and regeneration.

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