Opendata, web and dolomites

EV-LNC

Extracellular vesicle-mediated delivery of long non-coding RNA: Implications for vascular repair and regeneration

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 EV-LNC project word cloud

Explore the words cloud of the EV-LNC project. It provides you a very rough idea of what is the project "EV-LNC" about.

debris    cells    vectors    regeneration    methodology    vesicles    regulatory    data    cutting    lentiviral    crosstalk    molecules    fluorescent    imaging    guide    rna    recombination    therapy    cardiovascular    differentiation    triggered    integration    smooth    switch    mechanisms    offers    hypertension    driving    gapmers    colour    edge    drastically    initial    human    disease    intercellular    cargoes    molecular    seq    proteomics    interpret    evs    proliferation    gain    functional    cytometry    injury    biology    sirnas    comprise    muscle    repair    presume    background    brand    loxp    significantly    pulmonary    coding    model    respectively    signalling    lncrnas    transfer    implications    diseases    kinds    communication    endothelial    extracellular    vitro    paracrine    vascular    determinant    changing    flow    enriched    lncrna    significance    vesicle    elucidate    mediators    rnas    sorted    analysed    cre    changed    bioinformatics    ev    arterial    mediated    pioneer    fluorescence    demonstrated    relevance    mechanistic    macromolecules    function    cell    functions    besides   

Project "EV-LNC" data sheet

The following table provides information about the project.

Coordinator
THE UNIVERSITY OF EDINBURGH 

Organization address
address: OLD COLLEGE, SOUTH BRIDGE
city: EDINBURGH
postcode: EH8 9YL
website: www.ed.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Project website https://ec.europa.eu/research/participants/portal/desktop/en/projects/details.html
 Total cost 195˙454 €
 EC max contribution 195˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-08-01   to  2019-07-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE UNIVERSITY OF EDINBURGH UK (EDINBURGH) coordinator 195˙454.00

Map

 Project objective

Background: The concept of extracellular vesicles (EVs) has drastically changed from the initial non-functional debris to the current of key mediators of paracrine signalling. The cargoes of EVs comprise all kinds of macromolecules, and recent evidence has demonstrated the presence of long non-coding RNAs (lncRNAs) in such vesicles. These RNA molecules have numerous potential regulatory functions and results obtained so far guide us to presume a determinant role in vascular cell differentiation, proliferation and repair. Besides, the increasing data emerging in the field are significantly changing the way in which we interpret molecular mechanisms driving cardiovascular diseases and offers a brand new set of molecular targets for therapy. For all these reasons, study of lncRNAs in vascular biology and disease is state-of-the-art. Objectives: The main aim of this project is to study extracellular vesicle mediated cell-to-cell communication between human smooth muscle cells and endothelial cells, evaluate its relevance in vascular injury in an in vitro model of pulmonary arterial hypertension, and determine the significance of long non-coding RNA in this crosstalk. Methodology: For imaging EV transfer among vascular cells, we will use a pioneer approach based on Cre-loxP recombination which results in a fluorescent colour switch of cells upon EV uptake. Cells will then be sorted according to fluorescence by flow cytometry and analysed by cutting-edge proteomics and bioinformatics, in order to elucidate intercellular signalling triggered by EV transfer. The lncRNAs present in EVs will be analysed by RNA-Seq. Mechanistic insight of enriched lncRNAs in EVs will be evaluated using gain- and loss-of function approaches in vascular cells using lentiviral vectors and GapmeRs/siRNAs, respectively. Integration of all these analyses will provide key information to define implications of EV-mediated delivery of lncRNA for vascular repair and regeneration.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "EV-LNC" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "EV-LNC" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

Widow Spider Mating (2020)

Immature mating as a novel tactic of an invasive widow spider

Read More  

GLORIOUS (2019)

Digital Poetry in Today’s Russia: Canonisation and Translation

Read More  

SymCO (2020)

Asymptotic Symmetries: from Concepts to Observations

Read More