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Fluidblasto

Luminal pressure: a sculptor for mouse blastocyst self-organisation

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 Fluidblasto project word cloud

Explore the words cloud of the Fluidblasto project. It provides you a very rough idea of what is the project "Fluidblasto" about.

expression    cavity    inner    an    heterogeneity    map    ablation    imaging    morphogenetic    types    light    cytoskeletal    pressure    nascent    coalesce    mammalian    fate    blastocoel    remodeling    differentiation    uncharacterized    aspiration    induces    self    expansion    begins    filled    principles    signaling    technique    microscopy    cellular    mass    patterning    functionally    dynamic    mechanics    interdisciplinary    little    specification    organization    sufficient    event    biochemical    temporal    size    tissue    polarisation    progressively    resolution    sheet    blastocyst    cavities    morphogenesis    lineage    form    cell    luminal    micropressure    combining    live    epiblast    micropipette    roles    segregation    driving    physical    embryogenesis    intercellular    governing    interplay    cells    vivo    polarity    implantation    laser    manipulate    segregating    enhances    emergence    primitive    establishment    shape    quantitative    spatio    forces    elucidate    gene    fluid    quantify    endoderm    trophectoderm    adhesion    embryo    characterised    apical    multiple   

Project "Fluidblasto" data sheet

The following table provides information about the project.

Coordinator
EUROPEAN MOLECULAR BIOLOGY LABORATORY 

Organization address
address: Meyerhofstrasse 1
city: HEIDELBERG
postcode: 69117
website: http://www.embl.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 171˙460 €
 EC max contribution 171˙460 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-09-01   to  2020-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    EUROPEAN MOLECULAR BIOLOGY LABORATORY DE (HEIDELBERG) coordinator 171˙460.00

Map

 Project objective

An important morphogenetic event of mammalian embryogenesis is the formation of a blastocyst with a fluid-filled cavity, blastocoel, and the establishment of three cell types essential for implantation. Morphogenesis of the blastocyst begins with the emergence of multiple nascent cavities, which progressively coalesce to form one cavity segregating the cavity-facing primitive endoderm from the epiblast within the inner cell mass. While cell-to-cell gene expression heterogeneity is well characterised during this lineage specification, little is known about the physical principles governing self-organized blastocyst morphogenesis and patterning. In particular, changes in fluid pressure, cell shape and polarity during blastocyst formation remain uncharacterized. In this project, I will study the roles of fluid cavities in coordinating tissue mechanics, polarity and lineage specification. I will establish a novel micropressure technique to quantify the growth of luminal pressure during blastocyst development. Combining micropipette aspiration with high-resolution live-embryo imaging, I will characterize the impact of fluid pressure on trophectoderm fate specification through dynamic changes in cell shape and adhesion, and cytoskeletal remodeling. To assess the impact of fluid pressure on inner cell mass, I will study if cavity expansion induces apical polarisation and enhances primitive endoderm differentiation in cavity-facing cells. Combining laser ablation with light-sheet microscopy, we will build a spatio-temporal map of intercellular forces in vivo during blastocyst development. We will further manipulate the cavity size to study if fluid pressure is functionally required and sufficient for driving lineage segregation. This interdisciplinary and quantitative study will establish the novel role of fluid cavities and elucidate their interplay with biochemical signaling within the multi-cellular self-organization process.

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The information about "FLUIDBLASTO" are provided by the European Opendata Portal: CORDIS opendata.

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