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Fluidblasto

Luminal pressure: a sculptor for mouse blastocyst self-organisation

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 Fluidblasto project word cloud

Explore the words cloud of the Fluidblasto project. It provides you a very rough idea of what is the project "Fluidblasto" about.

roles    characterised    tissue    differentiation    forces    begins    remodeling    ablation    pressure    specification    expression    progressively    emergence    fluid    mammalian    heterogeneity    cavities    luminal    patterning    manipulate    live    principles    intercellular    cells    microscopy    laser    cellular    size    combining    induces    shape    uncharacterized    aspiration    interdisciplinary    map    expansion    imaging    quantify    an    quantitative    driving    elucidate    little    enhances    micropressure    mass    trophectoderm    polarity    apical    form    coalesce    morphogenesis    inner    resolution    interplay    technique    biochemical    self    embryo    blastocoel    implantation    cavity    segregation    embryogenesis    spatio    epiblast    gene    blastocyst    filled    sufficient    cytoskeletal    sheet    lineage    adhesion    fate    primitive    establishment    temporal    light    nascent    signaling    cell    mechanics    governing    vivo    organization    physical    functionally    polarisation    dynamic    endoderm    types    morphogenetic    micropipette    event    segregating    multiple   

Project "Fluidblasto" data sheet

The following table provides information about the project.

Coordinator
EUROPEAN MOLECULAR BIOLOGY LABORATORY 

Organization address
address: Meyerhofstrasse 1
city: HEIDELBERG
postcode: 69117
website: http://www.embl.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 171˙460 €
 EC max contribution 171˙460 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-09-01   to  2020-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    EUROPEAN MOLECULAR BIOLOGY LABORATORY DE (HEIDELBERG) coordinator 171˙460.00

Map

 Project objective

An important morphogenetic event of mammalian embryogenesis is the formation of a blastocyst with a fluid-filled cavity, blastocoel, and the establishment of three cell types essential for implantation. Morphogenesis of the blastocyst begins with the emergence of multiple nascent cavities, which progressively coalesce to form one cavity segregating the cavity-facing primitive endoderm from the epiblast within the inner cell mass. While cell-to-cell gene expression heterogeneity is well characterised during this lineage specification, little is known about the physical principles governing self-organized blastocyst morphogenesis and patterning. In particular, changes in fluid pressure, cell shape and polarity during blastocyst formation remain uncharacterized. In this project, I will study the roles of fluid cavities in coordinating tissue mechanics, polarity and lineage specification. I will establish a novel micropressure technique to quantify the growth of luminal pressure during blastocyst development. Combining micropipette aspiration with high-resolution live-embryo imaging, I will characterize the impact of fluid pressure on trophectoderm fate specification through dynamic changes in cell shape and adhesion, and cytoskeletal remodeling. To assess the impact of fluid pressure on inner cell mass, I will study if cavity expansion induces apical polarisation and enhances primitive endoderm differentiation in cavity-facing cells. Combining laser ablation with light-sheet microscopy, we will build a spatio-temporal map of intercellular forces in vivo during blastocyst development. We will further manipulate the cavity size to study if fluid pressure is functionally required and sufficient for driving lineage segregation. This interdisciplinary and quantitative study will establish the novel role of fluid cavities and elucidate their interplay with biochemical signaling within the multi-cellular self-organization process.

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The information about "FLUIDBLASTO" are provided by the European Opendata Portal: CORDIS opendata.

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