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Fluidblasto

Luminal pressure: a sculptor for mouse blastocyst self-organisation

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 Fluidblasto project word cloud

Explore the words cloud of the Fluidblasto project. It provides you a very rough idea of what is the project "Fluidblasto" about.

specification    quantitative    biochemical    progressively    coalesce    cytoskeletal    mechanics    polarisation    imaging    technique    laser    cellular    quantify    emergence    induces    apical    dynamic    segregation    morphogenetic    interdisciplinary    live    embryo    types    differentiation    manipulate    intercellular    polarity    physical    enhances    sheet    elucidate    inner    primitive    an    cavity    implantation    remodeling    fluid    fate    size    nascent    event    sufficient    mass    uncharacterized    driving    tissue    characterised    cell    trophectoderm    embryogenesis    cells    map    blastocyst    spatio    segregating    micropressure    roles    patterning    filled    endoderm    aspiration    gene    forces    temporal    ablation    mammalian    heterogeneity    adhesion    lineage    expression    epiblast    pressure    light    morphogenesis    interplay    signaling    form    principles    organization    little    expansion    multiple    blastocoel    micropipette    cavities    establishment    self    vivo    governing    functionally    luminal    shape    combining    resolution    microscopy    begins   

Project "Fluidblasto" data sheet

The following table provides information about the project.

Coordinator
EUROPEAN MOLECULAR BIOLOGY LABORATORY 

Organization address
address: Meyerhofstrasse 1
city: HEIDELBERG
postcode: 69117
website: http://www.embl.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 171˙460 €
 EC max contribution 171˙460 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-09-01   to  2020-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    EUROPEAN MOLECULAR BIOLOGY LABORATORY DE (HEIDELBERG) coordinator 171˙460.00

Map

 Project objective

An important morphogenetic event of mammalian embryogenesis is the formation of a blastocyst with a fluid-filled cavity, blastocoel, and the establishment of three cell types essential for implantation. Morphogenesis of the blastocyst begins with the emergence of multiple nascent cavities, which progressively coalesce to form one cavity segregating the cavity-facing primitive endoderm from the epiblast within the inner cell mass. While cell-to-cell gene expression heterogeneity is well characterised during this lineage specification, little is known about the physical principles governing self-organized blastocyst morphogenesis and patterning. In particular, changes in fluid pressure, cell shape and polarity during blastocyst formation remain uncharacterized. In this project, I will study the roles of fluid cavities in coordinating tissue mechanics, polarity and lineage specification. I will establish a novel micropressure technique to quantify the growth of luminal pressure during blastocyst development. Combining micropipette aspiration with high-resolution live-embryo imaging, I will characterize the impact of fluid pressure on trophectoderm fate specification through dynamic changes in cell shape and adhesion, and cytoskeletal remodeling. To assess the impact of fluid pressure on inner cell mass, I will study if cavity expansion induces apical polarisation and enhances primitive endoderm differentiation in cavity-facing cells. Combining laser ablation with light-sheet microscopy, we will build a spatio-temporal map of intercellular forces in vivo during blastocyst development. We will further manipulate the cavity size to study if fluid pressure is functionally required and sufficient for driving lineage segregation. This interdisciplinary and quantitative study will establish the novel role of fluid cavities and elucidate their interplay with biochemical signaling within the multi-cellular self-organization process.

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The information about "FLUIDBLASTO" are provided by the European Opendata Portal: CORDIS opendata.

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