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Fluidblasto

Luminal pressure: a sculptor for mouse blastocyst self-organisation

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 Fluidblasto project word cloud

Explore the words cloud of the Fluidblasto project. It provides you a very rough idea of what is the project "Fluidblasto" about.

combining    cavity    epiblast    form    polarity    endoderm    filled    interdisciplinary    segregating    types    size    mass    microscopy    begins    biochemical    shape    micropressure    blastocyst    spatio    elucidate    mammalian    mechanics    adhesion    roles    laser    establishment    lineage    characterised    patterning    morphogenesis    interplay    trophectoderm    physical    ablation    blastocoel    remodeling    segregation    fluid    heterogeneity    coalesce    enhances    emergence    technique    cytoskeletal    manipulate    vivo    tissue    induces    an    quantitative    uncharacterized    governing    expression    event    fate    quantify    cell    organization    forces    specification    functionally    embryo    pressure    embryogenesis    primitive    micropipette    morphogenetic    apical    light    nascent    signaling    sufficient    multiple    map    live    polarisation    cavities    progressively    self    expansion    principles    dynamic    temporal    sheet    little    driving    cellular    luminal    aspiration    cells    imaging    inner    implantation    resolution    differentiation    intercellular    gene   

Project "Fluidblasto" data sheet

The following table provides information about the project.

Coordinator
EUROPEAN MOLECULAR BIOLOGY LABORATORY 

Organization address
address: Meyerhofstrasse 1
city: HEIDELBERG
postcode: 69117
website: http://www.embl.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 171˙460 €
 EC max contribution 171˙460 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-09-01   to  2020-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    EUROPEAN MOLECULAR BIOLOGY LABORATORY DE (HEIDELBERG) coordinator 171˙460.00

Map

 Project objective

An important morphogenetic event of mammalian embryogenesis is the formation of a blastocyst with a fluid-filled cavity, blastocoel, and the establishment of three cell types essential for implantation. Morphogenesis of the blastocyst begins with the emergence of multiple nascent cavities, which progressively coalesce to form one cavity segregating the cavity-facing primitive endoderm from the epiblast within the inner cell mass. While cell-to-cell gene expression heterogeneity is well characterised during this lineage specification, little is known about the physical principles governing self-organized blastocyst morphogenesis and patterning. In particular, changes in fluid pressure, cell shape and polarity during blastocyst formation remain uncharacterized. In this project, I will study the roles of fluid cavities in coordinating tissue mechanics, polarity and lineage specification. I will establish a novel micropressure technique to quantify the growth of luminal pressure during blastocyst development. Combining micropipette aspiration with high-resolution live-embryo imaging, I will characterize the impact of fluid pressure on trophectoderm fate specification through dynamic changes in cell shape and adhesion, and cytoskeletal remodeling. To assess the impact of fluid pressure on inner cell mass, I will study if cavity expansion induces apical polarisation and enhances primitive endoderm differentiation in cavity-facing cells. Combining laser ablation with light-sheet microscopy, we will build a spatio-temporal map of intercellular forces in vivo during blastocyst development. We will further manipulate the cavity size to study if fluid pressure is functionally required and sufficient for driving lineage segregation. This interdisciplinary and quantitative study will establish the novel role of fluid cavities and elucidate their interplay with biochemical signaling within the multi-cellular self-organization process.

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The information about "FLUIDBLASTO" are provided by the European Opendata Portal: CORDIS opendata.

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