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Fluidblasto

Luminal pressure: a sculptor for mouse blastocyst self-organisation

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 Fluidblasto project word cloud

Explore the words cloud of the Fluidblasto project. It provides you a very rough idea of what is the project "Fluidblasto" about.

fluid    vivo    polarity    expression    functionally    little    live    mechanics    temporal    quantitative    enhances    manipulate    emergence    adhesion    nascent    aspiration    patterning    types    self    intercellular    roles    tissue    morphogenetic    technique    remodeling    shape    morphogenesis    primitive    driving    pressure    sufficient    uncharacterized    blastocyst    specification    spatio    cells    begins    segregating    elucidate    interdisciplinary    induces    cavities    differentiation    imaging    luminal    epiblast    sheet    cell    micropipette    polarisation    inner    coalesce    physical    forces    lineage    mass    filled    map    characterised    signaling    embryo    embryogenesis    trophectoderm    fate    blastocoel    mammalian    principles    quantify    size    endoderm    cytoskeletal    establishment    ablation    combining    microscopy    governing    laser    event    organization    heterogeneity    micropressure    apical    form    expansion    interplay    multiple    an    biochemical    gene    implantation    cavity    resolution    segregation    light    dynamic    cellular    progressively   

Project "Fluidblasto" data sheet

The following table provides information about the project.

Coordinator
EUROPEAN MOLECULAR BIOLOGY LABORATORY 

Organization address
address: Meyerhofstrasse 1
city: HEIDELBERG
postcode: 69117
website: http://www.embl.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 171˙460 €
 EC max contribution 171˙460 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-09-01   to  2020-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    EUROPEAN MOLECULAR BIOLOGY LABORATORY DE (HEIDELBERG) coordinator 171˙460.00

Map

 Project objective

An important morphogenetic event of mammalian embryogenesis is the formation of a blastocyst with a fluid-filled cavity, blastocoel, and the establishment of three cell types essential for implantation. Morphogenesis of the blastocyst begins with the emergence of multiple nascent cavities, which progressively coalesce to form one cavity segregating the cavity-facing primitive endoderm from the epiblast within the inner cell mass. While cell-to-cell gene expression heterogeneity is well characterised during this lineage specification, little is known about the physical principles governing self-organized blastocyst morphogenesis and patterning. In particular, changes in fluid pressure, cell shape and polarity during blastocyst formation remain uncharacterized. In this project, I will study the roles of fluid cavities in coordinating tissue mechanics, polarity and lineage specification. I will establish a novel micropressure technique to quantify the growth of luminal pressure during blastocyst development. Combining micropipette aspiration with high-resolution live-embryo imaging, I will characterize the impact of fluid pressure on trophectoderm fate specification through dynamic changes in cell shape and adhesion, and cytoskeletal remodeling. To assess the impact of fluid pressure on inner cell mass, I will study if cavity expansion induces apical polarisation and enhances primitive endoderm differentiation in cavity-facing cells. Combining laser ablation with light-sheet microscopy, we will build a spatio-temporal map of intercellular forces in vivo during blastocyst development. We will further manipulate the cavity size to study if fluid pressure is functionally required and sufficient for driving lineage segregation. This interdisciplinary and quantitative study will establish the novel role of fluid cavities and elucidate their interplay with biochemical signaling within the multi-cellular self-organization process.

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The information about "FLUIDBLASTO" are provided by the European Opendata Portal: CORDIS opendata.

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