HemTree2.0 SIGNED
Single cell genomic analysis and perturbations of hematopoietic progenitors: Towards a refined model of hematopoiesis
Total Cost €
0EC-Contrib. €
0Partnership
0Views
0HemTree2.0 project word cloud
Explore the words cloud of the HemTree2.0 project. It provides you a very rough idea of what is the project "HemTree2.0" about.
Project "HemTree2.0" data sheet
The following table provides information about the project.
| Coordinator |
WEIZMANN INSTITUTE OF SCIENCE
Organization address contact info |
| Coordinator Country | Israel [IL] |
| Total cost | 2˙000˙000 € |
| EC max contribution | 2˙000˙000 € (100%) |
| Programme |
1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC)) |
| Code Call | ERC-2016-COG |
| Funding Scheme | ERC-COG |
| Starting year | 2017 |
| Duration (year-month-day) | from 2017-10-01 to 2022-09-30 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | WEIZMANN INSTITUTE OF SCIENCE | IL (REHOVOT) | coordinator | 2˙000˙000.00 |
Map
Project objective
Hematopoiesis is an important model for stem cell differentiation with great medical significance. Heterogeneity within hematopoietic progenitor populations has considerably limited characterization and molecular understanding of lineage commitment in both health and disease. Advances in single-cell genomic technologies provide an extraordinary opportunity for unbiased and high resolution mapping of biological function and regulation. Recently we have developed an experimental and analytical method, termed massively parallel single-cell RNA-Seq (MARS-Seq), for unbiased classification of individual cells from their native context and successfully applied it for characterization of immune and hematopoietic progenitors.
Here, we propose to uncover the hierarchy and regulatory mechanisms controlling hematopoiesis by combining comprehensive single-cell RNA-Seq analyses, modelling approaches, advanced functional assays, single-cell CRISPR screens, knockout models and epigenetic profiling. Exciting preliminary result show that indeed our approach is starting to uncover the complexity of hematopoietic progenitors and the regulatory circuits driving hematopoietic decisions. We will pursue the following aims: (i) Generate a refined model of hematopoiesis by comprehensive single-cell RNA-Seq profiling of hematopoietic progenitors, (ii) validate the predicted model by in vivo functional developmental assays and then (iii) test candidate transcription and chromatin factors uncovered by our model for their role in controlling progression towards various lineages using single-cell measurements combined with CRISPR screens. Together, our study is expected to generate a revised and high-resolution hematopoietic model and decipher the regulatory networks that control hematopoiesis. Our methods and models may provide a platform for future medical advancements including a large-scale European collaborative project to discover a comprehensive human hematopoietic tree.
Publications
| year | authors and title | journal | last update |
|---|---|---|---|
| 2018 |
Amir Giladi, Ido Amit Single-Cell Genomics: A Stepping Stone for Future Immunology Discoveries published pages: 14-21, ISSN: 0092-8674, DOI: 10.1016/j.cell.2017.11.011 |
Cell 172/1-2 | 2019-06-06 |
| 2018 |
Guy Ledergor, Assaf Weiner, Mor Zada, Shuang-Yin Wang, Yael C. Cohen, Moshe E. Gatt, Nimrod Snir, Hila Magen, Maya Koren-Michowitz, Katrin Herzog-Tzarfati, Hadas Keren-Shaul, Chamutal Bornstein, Ron Rotkopf, Ido Yofe, Eyal David, Venkata Yellapantula, Sigalit Kay, Moshe Salai, Dina Ben Yehuda, Arnon Nagler, Lev Shvidel, Avi Orr-Urtreger, Keren Bahar Halpern, Shalev Itzkovitz, Ola Landgren, Jesus San-Miguel, Bruno Paiva, Jonathan J. Keats, Elli Papaemmanuil, Irit Avivi, Gabriel I. Barbash, Amos Tanay, Ido Amit Single cell dissection of plasma cell heterogeneity in symptomatic and asymptomatic myeloma published pages: 1867-1876, ISSN: 1078-8956, DOI: 10.1038/s41591-018-0269-2 |
Nature Medicine 24/12 | 2019-06-06 |
| 2017 |
Hadas Keren-Shaul, Amit Spinrad, Assaf Weiner, Orit Matcovitch-Natan, Raz Dvir-Szternfeld, Tyler K. Ulland, Eyal David, Kuti Baruch, David Lara-Astaiso, Beata Toth, Shalev Itzkovitz, Marco Colonna, Michal Schwartz, Ido Amit A Unique Microglia Type Associated with Restricting Development of Alzheimer’s Disease published pages: 1276-1290.e17, ISSN: 0092-8674, DOI: 10.1016/j.cell.2017.05.018 |
Cell 169/7 | 2019-06-06 |
| 2018 |
Amir Giladi, Franziska Paul, Yoni Herzog, Yaniv Lubling, Assaf Weiner, Ido Yofe, Diego Jaitin, Nina Cabezas-Wallscheid, Regine Dress, Florent Ginhoux, Andreas Trumpp, Amos Tanay, Ido Amit Single-cell characterization of haematopoietic progenitors and their trajectories in homeostasis and perturbed haematopoiesis published pages: 836-846, ISSN: 1465-7392, DOI: 10.1038/s41556-018-0121-4 |
Nature Cell Biology 20/7 | 2019-06-06 |
| 2019 |
Ronnie Blecher-Gonen, Pierre Bost, Kerry L. Hilligan, Eyal David, Tomer Meir Salame, Elsa Roussel, Lisa M. Connor, Johannes U. Mayer, Keren Bahar Halpern, Beáta Tóth, Shalev Itzkovitz, Benno Schwikowski, Franca Ronchese, Ido Amit Single-Cell Analysis of Diverse Pathogen Responses Defines a Molecular Roadmap for Generating Antigen-Specific Immunity published pages: 109-121.e6, ISSN: 2405-4712, DOI: 10.1016/j.cels.2019.01.001 |
Cell Systems 8/2 | 2019-06-06 |
| 2016 |
Diego Adhemar Jaitin, Assaf Weiner, Ido Yofe, David Lara-Astiaso, Hadas Keren-Shaul, Eyal David, Tomer Meir Salame, Amos Tanay, Alexander van Oudenaarden, Ido Amit Dissecting Immune Circuits by Linking CRISPR-Pooled Screens with Single-Cell RNA-Seq published pages: 1883-1896.e15, ISSN: 0092-8674, DOI: 10.1016/j.cell.2016.11.039 |
Cell 167/7 | 2019-06-06 |
| 2019 |
Hanjie Li, Anne M. van der Leun, Ido Yofe, Yaniv Lubling, Dikla Gelbard-Solodkin, Alexander C.J. van Akkooi, Marlous van den Braber, Elisa A. Rozeman, John B.A.G. Haanen, Christian U. Blank, Hugo M. Horlings, Eyal David, Yael Baran, Akhiad Bercovich, Aviezer Lifshitz, Ton N. Schumacher, Amos Tanay, Ido Amit Dysfunctional CD8 T Cells Form a Proliferative, Dynamically Regulated Compartment within Human Melanoma published pages: 775-789.e18, ISSN: 0092-8674, DOI: 10.1016/j.cell.2018.11.043 |
Cell 176/4 | 2019-06-06 |
| 2018 |
Merav Cohen, Amir Giladi, Anna-Dorothea Gorki, Dikla Gelbard Solodkin, Mor Zada, Anastasiya Hladik, Andras Miklosi, Tomer-Meir Salame, Keren Bahar Halpern, Eyal David, Shalev Itzkovitz, Tibor Harkany, Sylvia Knapp, Ido Amit Lung Single-Cell Signaling Interaction Map Reveals Basophil Role in Macrophage Imprinting published pages: 1031-1044.e18, ISSN: 0092-8674, DOI: 10.1016/j.cell.2018.09.009 |
Cell 175/4 | 2019-06-06 |
| 2018 |
Chamutal Bornstein, Shir Nevo, Amir Giladi, Noam Kadouri, Marie Pouzolles, François Gerbe, Eyal David, Alice Machado, Anna Chuprin, Beáta Tóth, Ori Goldberg, Shalev Itzkovitz, Naomi Taylor, Philippe Jay, Valérie S. Zimmermann, Jakub Abramson, Ido Amit Single-cell mapping of the thymic stroma identifies IL-25-producing tuft epithelial cells published pages: 622-626, ISSN: 0028-0836, DOI: 10.1038/s41586-018-0346-1 |
Nature 559/7715 | 2019-06-06 |
| 2017 |
Chiara Medaglia, Amir Giladi, Liat Stoler-Barak, Marco De Giovanni, Tomer Meir Salame, Adi Biram, Eyal David, Hanjie Li, Matteo Iannacone, Ziv Shulman, Ido Amit Spatial reconstruction of immune niches by combining photoactivatable reporters and scRNA-seq published pages: 1622-1626, ISSN: 0036-8075, DOI: 10.1126/science.aao4277 |
Science 358/6370 | 2019-06-06 |
Are you the coordinator (or a participant) of this project? Plaese send me more information about the "HEMTREE2.0" project.
For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.
Send me an email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.
Thanks. And then put a link of this page into your project's website.
The information about "HEMTREE2.0" are provided by the European Opendata Portal: CORDIS opendata.