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RNActivate SIGNED

Optochemical control of cell fate by activation of mRNA translation

Total Cost €

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EC-Contrib. €

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Partnership

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 RNActivate project word cloud

Explore the words cloud of the RNActivate project. It provides you a very rough idea of what is the project "RNActivate" about.

enabled       respective    migration    organism    resolution    modification    unmodified    light    capped    limitations    interfering    turn    moieties    inducible    biomolecular    rna    triggered    ing    subpopulations    prime    wavelength    uncompromised    release    precision    functions    transient    transferring    switch    function    death    cells    small    form    efficiency    adenosylmethionine    responsive    engineering    translational    messenger    trigger    eukaryotic    vital    ectopic    excellent    regulatory    producing    tracking    remethylated    block    easily    removal    bulky    injected    exploits    lack    cosubstrate    biology    manipulate    proteins    optochemical    agents    stage    mrna    antisense    expression    analogs    explore    labeling    exogenous    activate    embryos    groups    synthetic    external    photo    overcome    organisms    gene    methyltransferase    canonical    genome    adomet    temporal    fate    caged    cap    cellular    responsible    translation    spatio    cultured    cell    locally    cage    zebrafish    conditional    efficient    chemical    single    restore    molecular    regulation    promiscuous    vivo    abrogate   

Project "RNActivate" data sheet

The following table provides information about the project.

Coordinator
WESTFAELISCHE WILHELMS-UNIVERSITAET MUENSTER 

Organization address
address: SCHLOSSPLATZ 2
city: Munster
postcode: 48149
website: www.uni-muenster.de/en/

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 1˙990˙225 €
 EC max contribution 1˙990˙225 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-COG
 Funding Scheme ERC-COG
 Starting year 2018
 Duration (year-month-day) from 2018-06-01   to  2023-05-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    WESTFAELISCHE WILHELMS-UNIVERSITAET MUENSTER DE (Munster) coordinator 1˙990˙225.00

Map

 Project objective

Light is an excellent external regulatory element that can be applied to cells and organisms with high spatio-temporal precision and without interfering with cellular processes. Optochemical biology exploits small photo-responsive chemical groups to cage and activate or to switch biomolecular functions in response to light of a defined wavelength. Caged antisense agents have enabled down-regulation of gene expression with spatio-temporal control at the messenger-RNA (mRNA) level in vivo, however approaches for triggering translation of exogenous mRNA lack efficient turn-on effects. To explore the effects of conditional and transient ectopic gene expression in a developing organism it is vital to fully abrogate and restore translational efficiency.

The goal of this project is to bring eukaryotic mRNA under the control of light to trigger efficient ectopic translation with spatio-temporal resolution in cells and in vivo. To achieve this, eukaryotic mRNA will be photo-caged at its 5′ cap using a highly promiscuous methyltransferase capable of transferring very bulky moieties from synthetic analogs of the cosubstrate S-adenosylmethionine (AdoMet). A single 5′ cap modification will block translation of the respective mRNA. Its light-triggered removal will release unmodified capped RNA, which in cells will be efficiently remethylated to form the canonical 5′ cap resulting in uncompromised translation.

In addition to labeling and tracking subpopulations of cells, we will use our technology to control and to manipulate cell fate by locally producing proteins responsible for cell death, genome engineering, and cell migration. We will use cultured cells and one-cell stage zebrafish embryos that can be easily injected with mRNA to study the function of ectopic gene expression in early development. Our approach will overcome current limitations of photo-inducible mRNA translation and enable us to manipulate a developing organism at the molecular level.

 Publications

year authors and title journal last update
List of publications.
2019 Lea Anhäuser, Nils Klöcker, Fabian Muttach, Florian Mäsing, Petr Špaček, Armido Studer, Andrea Rentmeister
A Benzophenone-Based Photocaging Strategy for the N7 Position of Guanosine
published pages: , ISSN: 1433-7851, DOI: 10.1002/anie.201914573
Angewandte Chemie International Edition 2020-02-04
2020 Nicolas V. Cornelissen, Freideriki Michailidou, Fabian Muttach, Kristina Rau, Andrea Rentmeister
Nucleoside-modified AdoMet analogues for differential methyltransferase targeting
published pages: , ISSN: 1359-7345, DOI: 10.1039/c9cc07807j
Chemical Communications 2020-01-29

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The information about "RNACTIVATE" are provided by the European Opendata Portal: CORDIS opendata.

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