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High-Throughput Cryogenic Spectroscopy for Glycan Analysis

Total Cost €


EC-Contrib. €






 GLYCANAL project word cloud

Explore the words cloud of the GLYCANAL project. It provides you a very rough idea of what is the project "GLYCANAL" about.

glycoscience    database    adding    constructing    ims    dna    flight    simultaneous    glycan    easily    demonstrated    species    tool    world    attachment    dimension    additional    play    points    monosaccharide    difficult    bond    cellular    tremendous    separation    provides    distinguish    instrument    vibrational    throughput    isomerism    analyze    proteins    ms    isomerisms    virtually    building    distinguishes    disease    anomers    designing    mobility    hampers    mass    blocks    fingerprints    spectrum    time    primary    recognition    glycans    oligosaccharides    glycosidic    multiplex    combined    regioisomers    despite    simultaneously    occurrence    spectroscopic    stereochemistry    cells    decorate    data    structures    record    breakthrough    difficulty    lags    analytical    characterization    ubiquitous    isomeric    perform    mixture    complexity    spectrometry    structure    biological    sensitive    multiple    combines    types    infrared    surface    cryogenic    possibility    disaccharides    tabulated    intrinsic    branched    implicated    spectroscopy    nature    ion    almost   

Project "GLYCANAL" data sheet

The following table provides information about the project.


Organization address
address: BATIMENT CE 3316 STATION 1
postcode: 1015

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Total cost 2˙499˙801 €
 EC max contribution 2˙499˙801 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-ADG
 Funding Scheme ERC-ADG
 Starting year 2018
 Duration (year-month-day) from 2018-09-01   to  2023-08-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

Glycans, or oligosaccharides, are ubiquitous in biological systems. Because they decorate the surface of cells, they play a key role in virtually all cellular recognition processes and are implicated in almost every major disease. Despite their importance, the characterization of glycan primary structure lags far behind that of proteins and DNA because of their intrinsic isomeric complexity. The isomeric nature of the monosaccharide building blocks, the stereochemistry of the glycosidic bond, the possibility of multiple attachment points, and the occurrence of isomeric branched structures all make glycans difficult to analyze.

Although mass spectrometry (MS) is one of the most sensitive approaches for glycan analysis, it has difficulty to distinguish all these various types of isomerisms. Ion mobility spectrometry (IMS) combined with MS has demonstrated some ability to identify glycan anomers and regioisomers, but cannot easily distinguish isomeric disaccharides, for example.

We have recently demonstrated that cryogenic infrared spectroscopy provides unique vibrational fingerprints of glycans that distinguishes all the various types of isomerism. When combined with simultaneous measurements of mass and ion mobility, these fingerprints can be tabulated in a database and used to identify a given glycan from a mixture. However, adding a spectroscopic dimension to ion mobility and mass measurements requires additional time, which hampers it use as an analytical tool. To use spectroscopic data for real-world glycan analysis, one must multiplex the measurement process and record the vibrational spectrum of many species simultaneously.

This project involves designing and constructing an instrument that combines state-of-the-art ion mobility separation, cryogenic ion spectroscopy, and time-of-flight mass spectrometry to perform high throughput analysis of glycan primary structure. The success of this project would represent a tremendous breakthrough for glycoscience.


year authors and title journal last update
List of publications.
2019 Stephan Warnke, Ahmed Ben Faleh, Robert P. Pellegrinelli, Natalia Yalovenko, Thomas R. Rizzo
Combining ultra-high resolution ion mobility spectrometry with cryogenic IR spectroscopy for the study of biomolecular ions
published pages: 114-125, ISSN: 1359-6640, DOI: 10.1039/c8fd00180d
Faraday Discussions 217 2020-04-24
2019 Stephan Warnke, Ahmed Ben Faleh, Valeriu Scutelnic, Thomas R. Rizzo
Separation and Identification of Glycan Anomers Using Ultrahigh-Resolution Ion-Mobility Spectrometry and Cryogenic Ion Spectroscopy
published pages: 2204-2211, ISSN: 1044-0305, DOI: 10.1007/s13361-019-02333-0
Journal of The American Society for Mass Spectrometry 30/11 2020-04-24
2020 Robert P. Pellegrinelli, Lei Yue, Eduardo Carrascosa, Stephan Warnke, Ahmed Ben Faleh, Thomas R. Rizzo
How General Is Anomeric Retention during Collision-Induced Dissociation of Glycans?
published pages: 5948-5951, ISSN: 0002-7863, DOI: 10.1021/jacs.0c00264
Journal of the American Chemical Society 142/13 2020-04-24
2020 Irina Dyukova, Eduardo Carrascosa, Robert P. Pellegrinelli, Thomas R. Rizzo
Combining Cryogenic Infrared Spectroscopy with Selective Enzymatic Cleavage for Determining Glycan Primary Structure
published pages: 1658-1662, ISSN: 0003-2700, DOI: 10.1021/acs.analchem.9b04776
Analytical Chemistry 92/2 2020-04-24
2019 Ahmed Ben Faleh, Stephan Warnke, Thomas R. Rizzo
Combining Ultrahigh-Resolution Ion-Mobility Spectrometry with Cryogenic Infrared Spectroscopy for the Analysis of Glycan Mixtures
published pages: 4876-4882, ISSN: 0003-2700, DOI: 10.1021/acs.analchem.9b00659
Analytical Chemistry 91/7 2020-04-24

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