STEMCELLMARK

LGR receptors mark adult stem cells in multiple mammalian tissues

Coordinatore	KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPEN - KNAW    more Spiacenti, non ci sono informazioni su questo coordinatore. Contattare Fabio per maggiori infomrazioni, grazie.
Nazionalità Coordinatore	Netherlands [NL]
Totale costo	2˙106˙000 €
EC contributo	2˙106˙000 €
Programma	FP7-IDEAS-ERC Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	ERC-2008-AdG
Funding Scheme	ERC-AG
Anno di inizio	2009
Periodo (anno-mese-giorno)	2009-03-01   -   2014-02-28

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPEN - KNAW  Organization address address: KLOVENIERSBURGWAL 29 HET TRIPPENHUIS city: AMSTERDAM postcode: 1011 JV contact info Titolo: Mr. Nome: Don Cognome: Van Velzen Email: send email Telefono: +31 30 2121800 Fax: +31 30 2121865	NL (AMSTERDAM)	hostInstitution	2˙106˙000.00
2	KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPEN - KNAW  Organization address address: KLOVENIERSBURGWAL 29 HET TRIPPENHUIS city: AMSTERDAM postcode: 1011 JV contact info Titolo: Prof. Nome: Johannes Carolus Cognome: Clevers Email: send email Telefono: +31 30 2121831 Fax: +31 30 2121801	NL (AMSTERDAM)	hostInstitution	2˙106˙000.00

Mappa

 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

 Obiettivo del progetto (Objective)

'Self-renewal and repair of tissues in adult mammals is fueled by rare tissue stem cells. Identification and subsequent study of such adult stem cells depends strictly on the availability of molecular markers. To date, a paucity of single definitive markers has complicated the study of the biology of these rare cells. Our interest in the role of the Wnt pathway in intestinal homeostasis and cancer has led us to exhaustively define the Wnt target gene program shared between these two processes. Within this program, the cell surface receptor-encoding Lgr5 represented a candidate stem cell marker for the intestine. The creation of Lgr5-genetic mouse models subsequently allowed us to definitively identify the Lgr5 cells as the intestinal stem cells. These stem cells divide every day, yet persist throughout life. Using the same mouse models, we have found that Lgr5 marks stem cells in a variety of other tissues. For two family members with similarly restricted expression domains, Lgr4 and -6, comparable genetic tools have been generated. In this proposal, I aim to exploit these unique genetic tools to study primary adult stem cells in health and disease. This should allow us to outline unique and common characteristics of individual stem cell types, and possibly- define the minimum molecular determinants of stemness. Specifically, I aim: 1. To make a complete inventory of adult stem cells expressing Lgr4, Lgr5 and/or Lgr6 using the transgenic mouse models. 2. To FACS-purify stem cells from selected tissues, and detail their molecular and metabolic characteristics. 3. To genetically define the biological role of the Lgr-family receptors in adult stem cells by gene knockout. 4. To translate these findings to the equivalent human tissues using antibody-based histology and FACS technology. 5. To explore the usefulness of the Lgr proteins as markers of cancer stem cells in human solid tumors.'