BLIMP1 IN PGCS

Molecular Mechanism for Primordial Germ Cell Specification - The Role of Blimp1

 Coordinatore THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE 

 Organization address address: The Old Schools, Trinity Lane
city: CAMBRIDGE
postcode: CB2 1TN

contact info
Titolo: Ms.
Nome: Edna
Cognome: Murphy
Email: send email
Telefono: +44 1223 333543
Fax: +44 1223 332988

 Nazionalità Coordinatore United Kingdom [UK]
 Totale costo 0 €
 EC contributo 181˙350 €
 Programma FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call FP7-PEOPLE-IEF-2008
 Funding Scheme MC-IEF
 Anno di inizio 2009
 Periodo (anno-mese-giorno) 2009-06-01   -   2011-05-31

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE

 Organization address address: The Old Schools, Trinity Lane
city: CAMBRIDGE
postcode: CB2 1TN

contact info
Titolo: Ms.
Nome: Edna
Cognome: Murphy
Email: send email
Telefono: +44 1223 333543
Fax: +44 1223 332988

UK (CAMBRIDGE) coordinator 181˙350.77

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

fellow    critical    repressor    epiblast    pgcs    specification    mice    embryo    cells    mechanisms    pgc    genetic    pluripotency    fate    blimp    cell    mouse    germ    generate    stem   

 Obiettivo del progetto (Objective)

'Germ cells serve the critical function of transmitting genetic and epigenetic information across generations in multi-cellular organisms. An important attribute of the germ line is to generate the totipotent state. Studies of this unique cell lineage are of wide general interest in the context of stem cells and mechanisms of pluripotency. Relatively little is yet known about how germ cells are specified in mice. Recent genetic studies showed that the transcriptional repressor Blimp1 is a critical determinant of germ cell fate in the epiblast cells of the mouse embryo. There are only about 35 founder primordial germ cells (PGCs) present in each embryo, providing a significant hurdle for the mechanistic elucidation of germ cell specification. A robust in-vitro system that mimics PGC specification is essential to gain a deeper insight into the role of Blimp1 in germ cell fate determination. This project aims to generate such a system using a twofold approach. First, by engineering embryonic stem cells to express Blimp1 in an inducible manner and utilizing them for the induction of PGC fate. Second, by generating transgenic mice expressing fluorescent reporter molecules, for the derivation of epiblast stem cell cultures and subsequent FACS sorting of PGCs. To elucidate the gene expression programme governed by Blimp1 during PGC specification, the culture models will be used for analysis of the genome wide target site binding of Blimp1 and performing functional assays of the target genes and co-repressor proteins bound with Blimp1 to the sites identified. This will likely provide novel biochemical insight into PGC specification revealing mechanisms underlying the balance between pluripotency and differentiation. The project will provide training to the fellow in terms of techniques and subjects pertaining to stem cells, germ cells, mouse genetics and development, while at the same time allowing the re-integration of the fellow into the European scientific community.'

Altri progetti dello stesso programma (FP7-PEOPLE)

CONTROLNETONCTRANS (2009)

Identifying network control elements in breast cancer oncogenic transformation via whole transcriptome analysis

Read More  

ADINE (2012)

Art and Death in Neolithic Europe

Read More  

GHGELCAT (2013)

Electrocatalysis of greenhouse gases to fuels or chemical feedstocks on well-characterized materials

Read More