ICSC LGR5

Identification and Genetic Profile Characterization of Lgr5 positive Colon Cancer Stem Cells

 Coordinatore KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPEN - KNAW 

 Organization address address: KLOVENIERSBURGWAL 29 HET TRIPPENHUIS
city: AMSTERDAM
postcode: 1011 JV

contact info
Titolo: Mr.
Nome: Don
Cognome: Van Velzen
Email: send email
Telefono: +31 (0)30 2121800
Fax: +31 (0)30 2121865

 Nazionalità Coordinatore Netherlands [NL]
 Totale costo 0 €
 EC contributo 161˙162 €
 Programma FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call FP7-PEOPLE-IEF-2008
 Funding Scheme MC-IEF
 Anno di inizio 2009
 Periodo (anno-mese-giorno) 2009-05-01   -   2011-04-30

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPEN - KNAW

 Organization address address: KLOVENIERSBURGWAL 29 HET TRIPPENHUIS
city: AMSTERDAM
postcode: 1011 JV

contact info
Titolo: Mr.
Nome: Don
Cognome: Van Velzen
Email: send email
Telefono: +31 (0)30 2121800
Fax: +31 (0)30 2121865

NL (AMSTERDAM) coordinator 161˙162.47

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

crypt    expressed    intestine    marker    intestinal    gene    small    tumor    genes    cells    self    colon    tumour    tissues    normal    csc    positive    pattern    profile    compare    cancer    lgr    adult    epithelium    wnt    stem    cell    expression   

 Obiettivo del progetto (Objective)

'In the murine small intestine, the epithelium renews every five days. This vigorous self- renewing process is controlled by several secreted Wnt factors at the base of the crypt-villus axis. Lgr5/Gpr49 gene is a Wnt target gene that displays a complex expression pattern during embryogenesis, yet expression in most tissues subsides around birth. In adult mice, Lgr5 expression is restricted to rare, scattered cells in multiple tissues including the hair follicles, mammary glands and the intestinal epithelium. In the intestinal epithelium, Lgr5 is expressed throughout the proliferative crypt compartment and has been recently identified as a marker of adult stem cells in small intestine and colon. The cancer stem cell hypothesis postulates that a small reservoir of self-sustaining cells is exclusively able to self-renew and generate the heterogeneous cell population that constitutes the bulk of the tumour. Importantly, Cancer Stem Cells (CSC) not only fuel tumour growth but also tumor metastases. In 2002, Lgr5 was identified as a gene expressed in colon cancer. Therefore, the main goal of this project is to identify colon Cancer Stem Cells by using Lgr5 as a marker and compare the expression profile of this CSC cells with normal adult intestinal Stem Cells. First, I propose to analyse the Lgr5 expression pattern in tumours deriving from the intestinal tract, and compare it with the appropriate non-tumor controls. Following, Lgr5 positive cells will be isolated from fresh tumour suspensions, by antibody-based FACS sorting, and tested for Cancer Stem cell potential. Finally, the expression profile of Lgr5 positive colon Cancer Stem Cells will be compared with the genetic profile of Lgr5 normal intestinal Stem cells, to identify the genes implicated in Stem Cell malignant transformation. The results obtained from these studies would allow identifying potential target cells and target genes for the diagnosis and treatment of colon cancer.'

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