AOX1 PROMOTER

Purification and Characterization of Regulatory Proteins of the Alcohol Oxidase 1 (AOX1) Promoter of Pichia pastoris

Coordinatore	AKDENIZ UNIVERSITY    more  Organization address address: DUMLUPINAR BULVARI Kampus city: Antalya postcode: 7058 contact info Titolo: Prof. Nome: Muharrem Cognome: Certel Email: send email Telefono: -3102119 Fax: -2275536
Nazionalità Coordinatore	Turkey [TR]
Totale costo	100˙000 €
EC contributo	100˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2009-RG
Funding Scheme	MC-IRG
Anno di inizio	2010
Periodo (anno-mese-giorno)	2010-09-01   -   2014-08-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	AKDENIZ UNIVERSITY  Organization address address: DUMLUPINAR BULVARI Kampus city: Antalya postcode: 7058 contact info Titolo: Prof. Nome: Muharrem Cognome: Certel Email: send email Telefono: -3102119 Fax: -2275536	TR (Antalya)	coordinator	100˙000.00

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 Obiettivo del progetto (Objective)

'The methylotrophic yeast Pichia pastoris is used as a host system to produce recombinant proteins for a variety of applications, from food products to pharmaceuticals. However, despite the success of the P. pastoris expression system, relatively little is known about the molecular biology of the promoter utilized in this system, namely alcohol oxidase I (AOX1) promoter. In order to better utilize the AOX1 promoter, I propose to study regulation of the promoter using three approaches, 1) purify and characterize the regulatory proteins that bind to the AOX1 promoter and 2) overexpress his-tagged Methanol Expression Regulator 1 (Mrx1) protein to determine binding sites on the AOX1 promoter 3) determine the cis-acting regulatory sequences and design synthetic promoters. In addition, I will generate new host strains in which activator protein(s) will be over-expressed and expression of the repressor protein(s) will be suppressed. These studies will identify cis- and trans-acting factors that dictate the AOX1 promoter strength, in turn effect heterologous protein production. The elucidation of the AOX1 promoter control mechanism will benefit industrial and academic scientists who utilize P. pastoris expressions system in their studies.'

Introduzione (Teaser)

Researchers investigated how yeasts regulate protein production in order to create new strains with maximised protein yield.