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P4SB – From Plastic waste to Plastic value using Pseudomonas putida Synthetic Biology

Total Cost €


EC-Contrib. €






Project "P4SB" data sheet

The following table provides information about the project.


Organization address
city: AACHEN
postcode: 52062

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Project website
 Total cost 7˙056˙968 €
 EC max contribution 7˙056˙968 € (100%)
 Programme 1. H2020-EU.2.1.4. (INDUSTRIAL LEADERSHIP - Leadership in enabling and industrial technologies – Biotechnology)
 Code Call H2020-LEIT-BIO-2014-1
 Funding Scheme RIA
 Starting year 2015
 Duration (year-month-day) from 2015-04-01   to  2019-03-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
2    UNIVERSITAET LEIPZIG DE (LEIPZIG) participant 981˙875.00
3    BACMINE SL ES (TRES CANTOS) participant 684˙625.00
4    BIOPLASTECH LTD IE (DUBLIN) participant 628˙781.00
5    UNIVERSITY OF SURREY UK (GUILDFORD) participant 628˙125.00
10    SOPREMA FR (STRASBOURG) participant 317˙375.00
11    PROTEUS FR (LONGJUMEAU) participant 313˙625.00


 Project objective

P4SB is about the utilization of the conceptual and material tools of contemporary Synthetic Biology to bring about the sustainable and environmentally friendly bioconversion of oil-based plastic waste into fully biodegradable counterparts by means of deeply engineered, whole-cell bacterial catalysts. These tools will be used to design tailor-made enzymes for the bio-depolymerization of PET (polyethylene terephthalate) and PU (polyurethane), but also for the custom design of a Pseudomonas putida Cell Factory capable of metabolizing the resulting monomers. Pseudomonas putida will undergo deep metabolic surgery to channel these diverse substrates efficiently into the production of polyhydroxyalkanoates (PHA) and derivatives. In addition, synthetic downstream processing modules based on the programmed non-lytic secretion of PHA will facilitate the release and recovery of the bioplastic from the bacterial biomass.

These industry driven objectives will help to address the market need for novel routes to valorise the gigantic plastic waste streams in the European Union and beyond, with direct opportunities for SME partners of P4SB spanning the entire value chain from plastic waste via Synthetic Biology to biodegradable plastic. As a result we anticipate a completely biobased process reducing the environmental impact of plastic waste by establishing it as a novel bulk second generation carbon source for industrial biotechnology, while at the same time opening new opportunities for the European plastic recycling industry and helping to achieve the ambitious recycling targets set by the European Union for 2020.


List of deliverables.
Final report on scientific dissemination and exploitation of results incl. risk assessment, ethical, societal and intellectual property aspects Websites, patent fillings, videos etc. 2019-11-22 17:49:10
Interim Data Management Plan Open Research Data Pilot 2019-11-22 17:49:10
P4SB-project website with corporate identity Websites, patent fillings, videos etc. 2019-11-22 17:49:10
Initial Data Management Plan Open Research Data Pilot 2019-11-22 17:49:10
Interim report on scientific dissemination and exploitation of results incl. risk assessment, ethical, societal, and intellectual property aspects Websites, patent fillings, videos etc. 2019-11-22 17:49:09

Take a look to the deliverables list in detail:  detailed list of P4SB deliverables.


year authors and title journal last update
List of publications.
2019 Natalia A. Tarazona, Rainhard Machatschek, Burkhard Schulz, M. Auxiliadora Prieto, Andreas Lendlein
Molecular Insights into the Physical Adsorption of Amphiphilic Protein PhaF onto Copolyester Surfaces
published pages: TBD, ISSN: 1525-7797, DOI: 10.1021/acs.biomac.9b00069
Biomacromolecules 16 April 2019 2019-11-22
2019 Ren Wei, Daniel Breite, Chen Song, Daniel Gräsing, Tina Ploss, Patrick Hille, Ruth Schwerdtfeger, Jörg Matysik, Agnes Schulze, Wolfgang Zimmermann
Biocatalytic Degradation Efficiency of Postconsumer Polyethylene Terephthalate Packaging Determined by Their Polymer Microstructures
published pages: 1900491, ISSN: 2198-3844, DOI: 10.1002/advs.201900491
Advanced Science 2019-11-22
2019 Salvador, Abdulmutalib, Gonzalez, Kim, Smith, Faulon, Wei, Zimmermann, Jimenez
Microbial Genes for a Circular and Sustainable Bio-PET Economy
published pages: 373, ISSN: 2073-4425, DOI: 10.3390/genes10050373
Genes 10/5 2019-11-22
2017 Manuel S. Godoy, Beatrice Mongili, Debora Fino, M. Auxiliadora Prieto
About how to capture and exploit the CO 2 surplus that nature, per se, is not capable of fixing
published pages: 1216-1225, ISSN: 1751-7915, DOI: 10.1111/1751-7915.12805
Microbial Biotechnology 10/5 2019-11-22
2018 Siavash Atashgahi, Irene Sánchez-Andrea, Hermann J. Heipieper, Jan R. van der Meer, Alfons J. M. Stams, Hauke Smidt
Prospects for harnessing biocide resistance for bioremediation and detoxification
published pages: 743-746, ISSN: 0036-8075, DOI: 10.1126/science.aar3778
Science 360/6390 2019-11-22
2017 Tiso, Till; Zauter, Rabea; Tulke, Hannah; Leuchtle, Bernd; Li, Wing Jin; Behrens, Beate; Wittgens, Andreas; Rosenau, Frank; Hayen, Heiko; Blank, Lars Mathias
Designer rhamnolipids by reduction of congener diversity : production and characterization
published pages: 14;16(1):225, ISSN: 1475-2859, DOI: 10.18154/RWTH-2018-00735
Microbial cell factories 16, 225 (2017). doi:10.1186/s12934-017-0838-y 9 2019-11-22
2018 Mary Ann Franden, Lahiru N. Jayakody, Wing-Jin Li, Neil J. Wagner, Nicholas S. Cleveland, William E. Michener, Bernhard Hauer, Lars M. Blank, Nick Wierckx, Janosch Klebensberger, Gregg T. Beckham
Engineering Pseudomonas putida KT2440 for efficient ethylene glycol utilization
published pages: 197-207, ISSN: 1096-7176, DOI: 10.1016/j.ymben.2018.06.003
Metabolic Engineering 48 2019-11-22
2018 Tanja Narancic, Steven Verstichel, Srinivasa Reddy Chaganti, Laura Morales-Gamez, Shane T. Kenny, Bruno De Wilde, Ramesh Babu Padamati, Kevin E. O’Connor
Biodegradable Plastic Blends Create New Possibilities for End-of-Life Management of Plastics but They Are Not a Panacea for Plastic Pollution
published pages: , ISSN: 0013-936X, DOI: 10.1021/acs.est.8b02963
Environmental Science & Technology 2019-11-22
2018 Matheus Regis Belisário-Ferrari, Ren Wei, Tobias Schneider, Annett Honak, Wolfgang Zimmermann
Fast turbidimetric assay for analyzing the enzymatic hydrolysis of polyethylene terephthalate model substrates
published pages: 1800272, ISSN: 1860-6768, DOI: 10.1002/biot.201800272
Biotechnology Journal 2019-11-22
2018 Köbbing, Sebastian; Wierckx, Nick; Blank, Lars Mathias
Synthetic promoter stacking: When two plus two equals three
published pages: , ISSN: , DOI:
4th Meeting in Applied Synthetic Biology in Europe, Toulouse, France, 2018-10-24 - 2018-10-26 1 2019-11-22
2019 Audrey Magnin, Eric Pollet, Rémi Perrin, Christophe Ullmann, Cécile Persillon, Vincent Phalip, Luc Avérous
Enzymatic recycling of thermoplastic polyurethanes: Synergistic effect of an esterase and an amidase and recovery of building blocks
published pages: 141-150, ISSN: 0956-053X, DOI: 10.1016/j.wasman.2018.12.024
Waste Management 85 2019-11-22
2019 Natalia A. Tarazona, Beatriz Maestro, Olga Revelles, Jesús M. Sanz, M. Auxiliadora Prieto
Role of leucine zipper-like motifs in the oligomerization of Pseudomonas putida phasins
published pages: 362-370, ISSN: 0304-4165, DOI: 10.1016/j.bbagen.2018.11.002
Biochimica et Biophysica Acta (BBA) - General Subjects 1863/2 2019-11-22
2019 Aranzazu Mato, Natalia A. Tarazona, Alberto Hidalgo, Antonio Cruz, Mercedes Jiménez, Jesús Pérez-Gil, M. Auxiliadora Prieto
Interfacial Activity of Phasin PhaF from Pseudomonas putida KT2440 at Hydrophobic–Hydrophilic Biointerfaces
published pages: 678-686, ISSN: 0743-7463, DOI: 10.1021/acs.langmuir.8b03036
Langmuir 35/3 2019-11-22
2018 Alexander P. S. Darlington, Juhyun Kim, José I. Jiménez, Declan G. Bates
Engineering Translational Resource Allocation Controllers: Mechanistic Models, Design Guidelines, and Potential Biological Implementations
published pages: , ISSN: 2161-5063, DOI: 10.1021/acssynbio.8b00029
ACS Synthetic Biology 2019-11-22
2017 Rohola Hosseini, Jannis Kuepper, Sebastian Koebbing, Lars M. Blank, Nick Wierckx, Johannes H. de Winde
Regulation of solvent tolerance in Pseudomonas putida S12 mediated by mobile elements
published pages: n.a., ISSN: 1751-7915, DOI: 10.1111/1751-7915.12495
Microbial Biotechnology n.a. 2019-11-22
2018 Alexander P. S. Darlington, Juhyun Kim, José I. Jiménez, Declan G. Bates
Dynamic allocation of orthogonal ribosomes facilitates uncoupling of co-expressed genes
published pages: 695, ISSN: 2041-1723, DOI: 10.1038/s41467-018-02898-6
Nature Communications 9/1 2019-11-22
2018 Audrey Magnin, Lucie Hoornaert, Eric Pollet, Stéphanie Laurichesse, Vincent Phalip, Luc Avérous
Isolation and characterization of different promising fungi for biological waste management of polyurethanes
published pages: 1-12, ISSN: 1751-7915, DOI: 10.1111/1751-7915.13346
Microbial Biotechnology 2019-11-22
2018 Christian Eberlein, Thomas Baumgarten, Stephan Starke, Hermann J. Heipieper
Immediate response mechanisms of Gram-negative solvent-tolerant bacteria to cope with environmental stress: cis-trans isomerization of unsaturated fatty acids and outer membrane vesicle secretion
published pages: 2583-2593, ISSN: 0175-7598, DOI: 10.1007/s00253-018-8832-9
Applied Microbiology and Biotechnology 102/6 2019-11-22
2017 Tanja Narancic, Kevin E. O\'Connor
Microbial biotechnology addressing the plastic waste disaster
published pages: NA, ISSN: 1751-7915, DOI: 10.1111/1751-7915.12775
Microbial Biotechnology 2019-11-22
2016 J. Kim, M. Salvador, E. Saunders, J. Gonzalez, C. Avignone-Rossa, J. I. Jimenez
Properties of alternative microbial hosts used in synthetic biology: towards the design of a modular chassis
published pages: 303-313, ISSN: 0071-1365, DOI: 10.1042/EBC20160015
Essays In Biochemistry 60/4 2019-11-22
2017 Lars M. Blank
Let\'s talk about flux or the importance of (intracellular) reaction rates
published pages: 28-30, ISSN: 1751-7915, DOI: 10.1111/1751-7915.12455
Microbial Biotechnology 10/1 2019-11-22
2017 Matteo Cavaliere, Song Feng, Orkun Soyer, Jose I. Jimenez
Cooperation in microbial communities and their biotechnological applications
published pages: 2949-2963, ISSN: 1462-2912, DOI: 10.1111/1462-2920.13767
Environmental Microbiology 19(8) 2019-11-22
2016 Auxiliadora Prieto
To be, or not to be biodegradable… that is the question for the bio-based plastics
published pages: 652-657, ISSN: 1751-7915, DOI: 10.1111/1751-7915.12393
Microbial Biotechnology 9/5 2019-11-22
2015 Sebastian Zobel, Ilaria Benedetti, Lara Eisenbach, Victor de Lorenzo, Nick Wierckx, Lars M. Blank
Tn7-Based Device for Calibrated Heterologous Gene Expression in Pseudomonas putida
published pages: 1341-1351, ISSN: 2161-5063, DOI: 10.1021/acssynbio.5b00058
ACS Synthetic Biology 4/12 2019-11-22
2017 Jose Ignacio Jiménez
Shedding light on the black box models of the cell
published pages: 43-45, ISSN: 1751-7915, DOI: 10.1111/1751-7915.12489
Microbial Biotechnology 10 2019-11-22
2017 Ren Wei, Wolfgang Zimmermann
Biocatalysis as a green route for recycling the recalcitrant plastic polyethylene terephthalate
published pages: 6, ISSN: 1751-7915, DOI: 10.1111/1751-7915.12714
Microbial Biotechnology Vol. 10, 2017 2019-11-22
2017 Ren Wei, Wolfgang Zimmermann
Microbial enzymes for the recycling of recalcitrant petroleum-based plastics: how far are we?
published pages: , ISSN: 1751-7915, DOI: 10.1111/1751-7915.12710
Microbial Biotechnology Vol. 10, 2017 2019-11-22
2015 Nick Wierckx, M. Auxiliadora Prieto, Pablo Pomposiello, Victor de Lorenzo, Kevin O\'Connor, Lars M. Blank
Plastic waste as a novel substrate for industrial biotechnology
published pages: 900-903, ISSN: 1751-7915, DOI: 10.1111/1751-7915.12312
Microbial Biotechnology 8/6 2019-11-22
2016 Köbbing, Sebastian; Wynands, Benedikt Niklas Daniel; Blank, Lars Mathias; Wierckx, Nick
Synthetic promoter libraries, a powerful tool for tuning gene expression
published pages: , ISSN: , DOI:
3rd Congress on Applied Synthetic Biology in Europe, Lisbon, Portugal, 2016-02-22 - 2016-02-24 1 2019-11-22

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