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HAT-MOZ-sphere

Identification of genes and pathways regulated by the HAT activity of MOZ

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
results

HAT-MOZ-sphere project word cloud

Explore the words cloud of the HAT-MOZ-sphere project. It provides you a very rough idea of what is the project "HAT-MOZ-sphere" about.

moz line loci expertise enzymatic encoding fusion hat polymerase translocations transduction genes performed supervisor calibre host marks variety haematopoiesis skills leukaemia exceptionally mouse epigenetic cells anne deletion finger position seq recruitment powerful technique tool bone programs crucially acute player maintenance comprise mutated training murine histone function zinc dr modifications marrow transferable leadership obtain protein multidisciplinary leukaemic genome landscape relying proteins acetyl cancer rna manchester retrovirus regulated tif2 survey determined understand institute transferase critical sequencing leukaemogenesis model chromatin competitive abrogate uk followed silac haematopoietic chip immunoprecipitation monocytic largeot obtaining myeloblastic laboratory

Project "HAT-MOZ-sphere" data sheet

The following table provides information about the project.

Coordinator	THE UNIVERSITY OF MANCHESTER Organization address address: OXFORD ROAD city: MANCHESTER postcode: M13 9PL website: www.manchester.ac.uk contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	United Kingdom [UK]
Project website	http://www.cruk.manchester.ac.uk/Research/CRUK-MI-Groups/Stem-Cell-Biology/Home
Total cost	195˙454 €
EC max contribution	195˙454 € (100%)
Programme	1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
Code Call	H2020-MSCA-IF-2014
Funding Scheme	MSCA-IF-EF-ST
Starting year	2015
Duration (year-month-day)	from 2015-09-01 to 2017-08-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	THE UNIVERSITY OF MANCHESTER THE UNIVERSITY OF MANCHESTER Organization address address: OXFORD ROAD city: MANCHESTER postcode: M13 9PL website: www.manchester.ac.uk contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	UK (MANCHESTER)	coordinator	195˙454.00

Map

Project objective

The research programme will comprise the study of the Histone acetyl transferase (HAT) MOZ (Monocytic leukaemia Zinc finger) a critical player in the development and maintenance of the haematopoietic system. In addition, translocations of MOZ are associated with Acute Myeloblastic Leukaemia. Given the importance of this protein in haematopoiesis and leukaemogenesis, it would be essential to further understand the function of MOZ and in particular to identify the genes regulated by its HAT activity of MOZ. A mouse line specifically mutated to abrogate the HAT enzymatic activity of MOZ has been developed in the host laboratory, and represents a powerful and unique tool for the study of MOZ HAT activity. Using Chromatin ImmunoPrecipitation followed by sequencing (ChIP-seq), genome wide targets of MOZ will be determined and the consequences of the deletion of its HAT activity on the epigenetic landscape will be investigated through the survey of several histone modifications and RNA polymerase II recruitment. The non-histone targets of MOZ will be identified through the SILAC technique. Finally a MOZ murine leukaemic model has been developed, based on the transduction of bone marrow cells with a retrovirus encoding MOZ-TIF2, one of the fusion proteins involving MOZ. ChIP-seq will be performed in these leukaemic cells to identify genes targeted by MOZ-TIF2 and changes in epigenetic marks at these loci. Overall, this project will provide a better understanding of MOZ HAT activity in haematopoiesis and leukaemogenesis. Crucially, this project will enable Dr Anne Largeot to obtain and develop a wide range of new and transferable skills through a variety of multidisciplinary training programs. Relying on the knowledge and expertise of her supervisor and the exceptionally high calibre of the Cancer Research UK Manchester Institute, this project will enable Dr Anne Largeot to achieve her long-term objective of obtaining a leadership position in a competitive European Institute

Publications

List of publications.
year	authors and title	journal	last update
2016	Andrew J. Lilly, Guilherme Costa, Anne Largeot, Muhammad Z. H. Fadlullah, Michael Lie-A-Ling, Georges Lacaud, Valerie Kouskoff Interplay between SOX7 and RUNX1 regulates hemogenic endothelial fate in the yolk sac published pages: 4341-4351, ISSN: 0950-1991, DOI: 10.1242/dev.140970	Development 143/23	2019-06-14
2016	Anne Largeot, Flor Maria Perez-Campo, Elli Marinopoulou, Michael Lie-a-Ling, Valerie Kouskoff, Georges Lacaud Expression of the MOZ-TIF2 oncoprotein in mice represses senescence published pages: 231-237.e4, ISSN: 0301-472X, DOI: 10.1016/j.exphem.2015.12.006	Experimental Hematology 44/4	2019-06-14

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