Opendata, web and dolomites

PlasmoSilencing SIGNED

Exoribonuclease-mediated degradation of nascent RNA in Malaria Parasites: A Novel Mechanism in Virulence Gene Silencing

Total Cost €


EC-Contrib. €






 PlasmoSilencing project word cloud

Explore the words cloud of the PlasmoSilencing project. It provides you a very rough idea of what is the project "PlasmoSilencing" about.

made    approximately    genome    pfrnase    organisms    linked    cyclic    erc    transcriptional    deficient    relatively    human    grant    causes    transcription    occurs    elusive    children    200    deaths    immune    discovered    mechanism    mechanisms    blood    genes    full    cryptic    rna    mainstream    crispr    recruitment    events    promoter    noncoding    malaria    silences    assays    destroys    regulation    exoribonucleases    editing    total    entirely    protein    exoribonuclease    termed    activation    posttranscriptional    molecular    scherf    demonstrated    asexual    occupancy    length    nascent    evasion    million    paradigms    canonical    gene    few    appeal    repercussions    background    severe    monoallelic    monocistronic    strategies    types    laboratory    avenues    falciparum    mainly    parasite    coordinated    expression    parasites    dependent    stages    technique    exist    cas9    regulatory    silencing    untouched    mrna    join    regions    infections    protozoan    innovative    pull    young    unstable   

Project "PlasmoSilencing" data sheet

The following table provides information about the project.


Organization address
address: RUE MICHEL ANGE 3
city: PARIS
postcode: 75794

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Project website
 Total cost 2˙499˙761 €
 EC max contribution 2˙499˙761 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2014-ADG
 Funding Scheme ERC-ADG
 Starting year 2015
 Duration (year-month-day) from 2015-11-01   to  2020-10-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

Background: The human protozoan malaria parasite P. falciparum causes approximately 200 million infections and 0.7 million deaths (mainly children) per year. In the well-studied asexual blood stages, cyclic monocistronic gene activation occurs at the transcriptional level; however, relatively few transcription factors have been identified, thus other types of regulatory processes that contribute to this coordinated gene expression are believed to exist. Through the study of molecular process of monoallelic expression of immune evasion genes in P. falciparum (project funded by a previous ERC grant to A. Scherf), we discovered an entirely new mechanism of gene silencing. We demonstrated that an exoribonuclease silences genes linked to severe malaria. A non-canonical 3’-5’exoribonuclease termed PfRNase II destroys nascent RNA made from promoter regions, leading to cryptic unstable mRNA. Parasites carrying a deficient PfRNase II produce full-length mRNA and long noncoding RNA. The molecular events and the number of genes directly controlled by this novel type of posttranscriptional gene silencing remain elusive. Aim: This proposal aims to investigate the molecular mechanisms controlling PfRNase II-dependent gene silencing using innovative strategies such as the new genome editing technique (Cas9/CRISPR) developed in my laboratory for use in P. falciparum. We will study i) the recruitment of PfRNase II to promoter regions of severe malaria related genes using protein pull-down assays and ii) the genome occupancy of PfRNase II and two other 3’-5’ exoribonucleases to determine the total number of genes controlled by this mechanism. Impact: This project represents a major change in mainstream malaria parasite gene regulation paradigms with repercussions for other organisms. The proposed research will both open new avenues in molecular process that control severe malaria and appeal to young researchers to join this rather ‘untouched’ topic.


year authors and title journal last update
List of publications.
2016 Shruthi Sridhar Vembar, Matthew Seetin, Christine Lambert, Maria Nattestad, Michael C. Schatz, Primo Baybayan, Artur Scherf, Melissa Laird Smith
Complete telomere-to-telomere de novo assembly of the Plasmodium falciparum genome through long-read (>11 kb), single molecule, real-time sequencing
published pages: 339-351, ISSN: 1340-2838, DOI: 10.1093/dnares/dsw022
DNA Research 23/4 2019-07-05
2017 Jessica M. Bryant, Clément Regnault, Christine Scheidig-Benatar, Sebastian Baumgarten, Julien Guizetti, Artur Scherf
CRISPR/Cas9 Genome Editing Reveals That the Intron Is Not Essential for var2csa Gene Activation or Silencing in Plasmodium falciparum
published pages: , ISSN: 2150-7511, DOI: 10.1128/mBio.00729-17
mBio 8/4 2019-07-05
2018 Jessica M. Bryant, Sebastian Baumgarten, Audrey Lorthiois, Christine Scheidig-Benatar, Aurélie Claës, Artur Scherf
De Novo Genome Assembly of a Plasmodium falciparum NF54 Clone Using Single-Molecule Real-Time Sequencing
published pages: , ISSN: 2169-8287, DOI: 10.1128/genomeA.01479-17
Genome Announcements 6/5 2019-07-05
2018 Gigliola Zanghì, Shruthi S. Vembar, Sebastian Baumgarten, Shuai Ding, Julien Guizetti, Jessica M. Bryant, Denise Mattei, Anja T.R. Jensen, Laurent Rénia, Yun Shan Goh, Robert Sauerwein, Cornelus C. Hermsen, Jean-François Franetich, Mallaury Bordessoulles, Olivier Silvie, Valérie Soulard, Olivier Scatton, Patty Chen, Salah Mecheri, Dominique Mazier, Artur Scherf
A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection
published pages: 2951-2963, ISSN: 2211-1247, DOI: 10.1016/j.celrep.2018.02.075
Cell Reports 22/11 2019-07-05
2017 Rafael M. Martins, Cameron R. Macpherson, Aurélie Claes, Christine Scheidig-Benatar, Hiroshi Sakamoto, Xue Yan Yam, Peter Preiser, Suchi Goel, Mats Wahlgren, Odile Sismeiro, Jean-Yves Coppée, Artur Scherf
An ApiAP2 member regulates expression of clonally variant genes of the human malaria parasite Plasmodium falciparum
published pages: , ISSN: 2045-2322, DOI: 10.1038/s41598-017-12578-y
Scientific Reports 7/1 2019-07-05
2017 Fabien Sindikubwabo, Shuai Ding, Tahir Hussain, Philippe Ortet, Mohamed Barakat, Sebastian Baumgarten, Dominique Cannella, Andrés Palencia, Alexandre Bougdour, Lucid Belmudes, Yohann Couté, Isabelle Tardieux, Cyrille Y Botté, Artur Scherf, Mohamed-ali Hakimi
Modifications at K31 on the lateral surface of histone H4 contribute to genome structure and expression in apicomplexan parasites
published pages: , ISSN: 2050-084X, DOI: 10.7554/eLife.29391
eLife 6 2019-07-05
2016 Julien Guizetti, Anna Barcons-Simon, Artur Scherf
Trans-acting GC-rich non-coding RNA at var expression site modulates gene counting in malaria parasite
published pages: gkw664, ISSN: 0305-1048, DOI: 10.1093/nar/gkw664
Nucleic Acids Research 2019-07-05
2016 Shruthi Sridhar Vembar, Dorothea Droll, Artur Scherf
Translational regulation in blood stages of the malaria parasite Plasmodium spp. : systems-wide studies pave the way
published pages: 772-792, ISSN: 1757-7004, DOI: 10.1002/wrna.1365
Wiley Interdisciplinary Reviews: RNA 7/6 2019-07-05

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "PLASMOSILENCING" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email ( and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "PLASMOSILENCING" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

TroyCAN (2020)

Redefining the esophageal stem cell niche – towards targeting of squamous cell carcinoma

Read More  

InsideChromatin (2019)

Towards Realistic Modelling of Nucleosome Organization Inside Functional Chromatin Domains

Read More  

SuperH (2019)

Discovery and Characterization of Hydrogen-Based High-Temperature Superconductors

Read More