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AS-ABA SIGNED

Alternative splicing and SR proteins in ABA-mediated plant stress responses

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 AS-ABA project word cloud

Explore the words cloud of the AS-ABA project. It provides you a very rough idea of what is the project "AS-ABA" about.

generation    drought    action    serine    expressing    genes    genome    discovering    rip    levels    productivity    sr    molecular    central    mode    localization    revealing    phytohormone    tagged    technologies    reverse    regulation    alternative    unfavorable    diversity    functional    physiological    altered    mechanisms    sequencing    unknown    pave    stress    causes    abscisic    immunoprecipitation    splicing    combination    protein    expanding    abiotic    efficient    suggests    plays    environmental    aba    proteins    flexible    gain    cope    play    regulating    relevance    crop    genetics    version    plant    gene    arginine    acid    transcriptome    underlying    mounting    worldwide    six    transcripts    world    eukaryotes    mechanistic    strategies    of    subcellular    significance    tolerance    posttranscriptional    proteome    roles    implicated    mechanism    seq    regulated    pervasive    salinity    hormone    arabidopsis    brief    mediated    biochemical    largely    regions    rna    binding    expression    hormonal    plants   

Project "AS-ABA" data sheet

The following table provides information about the project.

Coordinator
FUNDACAO CALOUSTE GULBENKIAN 

Organization address
address: AVENIDA BERNA 45
city: LISBOA
postcode: 1000
website: www.igc.gulbenkian.pt

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Portugal [PT]
 Project website http://www.igc.gulbenkian.pt/pduque
 Total cost 148˙635 €
 EC max contribution 148˙635 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2015
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-09-01   to  2019-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    FUNDACAO CALOUSTE GULBENKIAN PT (LISBOA) coordinator 148˙635.00

Map

 Project objective

Abscisic acid (ABA), the major plant stress hormone, plays a crucial role in the response to the most pervasive causes of loss of crop productivity worldwide, including drought and high salinity. Serine/arginine-rich (SR) proteins are RNA-binding proteins that play crucial roles in the regulation of alternative splicing (AS), a key posttranscriptional mechanism for expanding proteome diversity and a flexible means of regulating gene expression in higher eukaryotes. While its functional relevance in plants remains largely unknown, mounting evidence suggests a central role for AS in the response to abiotic stress, in particular by targeting the ABA hormonal pathway. Using gene expression analyses, subcellular localization studies and reverse genetics approaches, this project will investigate the functional significance of six ABA-regulated Arabidopsis SR genes in stress responses mediated by the ABA phytohormone. To gain mechanistic insight into the mode of action of the SR proteins implicated in the ABA pathway, the physiological transcripts targeted by these splicing factors to achieve plant stress tolerance will be identified using a combination of genome-wide and biochemical approaches. In brief, next-generation sequencing technologies will be applied to whole transcriptome analysis (RNA-seq) of plants with altered levels of an SR protein and to RNA immunoprecipitation (RIP-seq) in plants expressing a tagged version of the SR protein. In discovering new plant genes that determine the ability to cope with unfavorable environmental conditions and by revealing the molecular mechanisms underlying their mode of action, this work will pave the way for the development of new efficient strategies to improve crop productivity in specific world regions.

 Publications

year authors and title journal last update
List of publications.
2018 Tom Laloum, Guiomar Martín, Paula Duque
Alternative Splicing Control of Abiotic Stress Responses
published pages: 140-150, ISSN: 1360-1385, DOI: 10.1016/j.tplants.2017.09.019
Trends in Plant Science 23/2 2019-10-31

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