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Totipotency SIGNED

Transcriptional and Epigenetic Regulation of Totipotency in Mouse Early Embryos.

Total Cost €

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EC-Contrib. €

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Partnership

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 Totipotency project word cloud

Explore the words cloud of the Totipotency project. It provides you a very rough idea of what is the project "Totipotency" about.

epigenetic    models    binding    reproductive    conditionally    immature    zga    molecular    human    totipotent    gametes    imaging    relevance    embryonic    depleting    erc    sites    significance    med    exquisite    combined    sophisticated    truly    conditional    fate    computational    possibly    nucleosomes    zygotic    maternally    nascent    mature    interrogate    candidate    regulators    later    developmental    parental    differentiated    transferring    gain    totipotency    manner    biology    dissecting    regulation    regulate    islands    sperm    underlying    gene    modifiers    embryos    genome    initiate    assisted    reprogramming    fusion    initiation    competence    landscapes    translation    deficiency    activation    paternal    gives    genomic    acquisition    wild    mammals    spermatogenesis    organism    degradation    histone    mechanisms    utilize    cell    nuclei    live    transcription    oocytes    coincides    contribution    chromatin    cpg    technologies    barely    germline    mouse    transcripts    deficient    inheritance    quantitative    modified    nucleosome    sensitive    global    spermatid    function    zygote    eviction    tfs   

Project "Totipotency" data sheet

The following table provides information about the project.

Coordinator
FRIEDRICH MIESCHER INSTITUTE FOR BIOMEDICAL RESEARCH FONDATION 

Organization address
address: MAULBEERSTRASSE 66
city: BASEL
postcode: 4058
website: www.fmi.ch

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Total cost 2˙859˙560 €
 EC max contribution 2˙859˙560 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2015-AdG
 Funding Scheme ERC-ADG
 Starting year 2016
 Duration (year-month-day) from 2016-08-01   to  2021-07-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    FRIEDRICH MIESCHER INSTITUTE FOR BIOMEDICAL RESEARCH FONDATION CH (BASEL) coordinator 2˙859˙560.00

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 Project objective

In mammals, fusion of two highly differentiated gametes gives rise to a totipotent zygote capable of developing into a whole organism. It coincides with translation and degradation of maternally provided transcripts, initiation of global transcription called zygotic genome activation (ZGA), and “epigenetic reprogramming” of germline chromatin states into an embryonic state. The molecular mechanisms underlying this exquisite reprogramming of cell fate are barely understood.

This research program has the ambitious goal to identify and characterize in a comprehensive way the transcription factors and chromatin regulators which initiate and regulate ZGA in a parental specific manner in early mouse embryos. We will utilize novel and highly sensitive genomic approaches to measure nascent transcription and determine open and modified chromatin landscapes in oocytes and early embryos, wild-type and conditionally deficient for major epigenetic modifiers. We will apply computational approaches to identify candidate TFs and histone modifiers controlling ZGA. We will use molecular and developmental biology approaches, combined with sensitive quantitative live-imaging, to interrogate the function of TFs and their binding sites for ZGA.

We will further investigate the significance of possible paternal inheritance of nucleosomes at CpG islands for gene regulation during ZGA and later development by depleting nucleosomes from mature sperm by using sophisticated conditional deficiency and gain-of-function mouse models. By transferring nuclei of immature spermatid and mature sperm into oocytes, we will interrogate the relevance of nucleosome eviction during spermatogenesis, as a possibly truly epigenetic reprogramming process, for defining embryonic competence.

ERC funding would represent a crucial contribution to dissecting the molecular mechanisms underlying acquisition of totipotency in mouse embryos and may impact on the use of Assisted Reproductive Technologies in human med

 Publications

year authors and title journal last update
List of publications.
2018 Mark E. Gill, Antoine H. F. M. Peters
Toward human egg-like cells in vitro
published pages: 291-292, ISSN: 0036-8075, DOI: 10.1126/science.aav3479
Science 362/6412 2019-06-03
2019 Maaike Welling, Manuel Alexander Mohr, Aaron Ponti, Lluc Rullan Sabater, Andrea Boni, Yumiko K Kawamura, Prisca Liberali, Antoine HFM Peters, Pawel Pelczar, Periklis Pantazis
Primed Track, high-fidelity lineage tracing in mouse pre-implantation embryos using primed conversion of photoconvertible proteins
published pages: , ISSN: 2050-084X, DOI: 10.7554/elife.44491
eLife 8 2019-06-03

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The information about "TOTIPOTENCY" are provided by the European Opendata Portal: CORDIS opendata.

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