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Totipotency SIGNED

Transcriptional and Epigenetic Regulation of Totipotency in Mouse Early Embryos.

Total Cost €


EC-Contrib. €






 Totipotency project word cloud

Explore the words cloud of the Totipotency project. It provides you a very rough idea of what is the project "Totipotency" about.

deficient    possibly    gene    developmental    imaging    technologies    initiate    maternally    epigenetic    erc    transcription    conditionally    nuclei    models    embryonic    quantitative    med    modified    initiation    regulation    immature    nucleosome    paternal    embryos    candidate    utilize    zygotic    genomic    tfs    oocytes    conditional    sensitive    human    totipotency    organism    activation    parental    spermatogenesis    binding    relevance    significance    function    mechanisms    spermatid    sperm    dissecting    zga    transcripts    computational    gametes    fate    totipotent    global    mouse    later    mature    manner    reprogramming    chromatin    exquisite    inheritance    live    acquisition    assisted    landscapes    sites    differentiated    fusion    barely    competence    interrogate    genome    germline    islands    biology    histone    translation    wild    coincides    zygote    regulators    nascent    sophisticated    cell    deficiency    nucleosomes    molecular    cpg    mammals    truly    depleting    degradation    gives    contribution    regulate    gain    eviction    reproductive    underlying    modifiers    transferring    combined   

Project "Totipotency" data sheet

The following table provides information about the project.


Organization address
city: BASEL
postcode: 4058

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Total cost 2˙859˙560 €
 EC max contribution 2˙859˙560 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2015-AdG
 Funding Scheme ERC-ADG
 Starting year 2016
 Duration (year-month-day) from 2016-08-01   to  2021-07-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

In mammals, fusion of two highly differentiated gametes gives rise to a totipotent zygote capable of developing into a whole organism. It coincides with translation and degradation of maternally provided transcripts, initiation of global transcription called zygotic genome activation (ZGA), and “epigenetic reprogramming” of germline chromatin states into an embryonic state. The molecular mechanisms underlying this exquisite reprogramming of cell fate are barely understood.

This research program has the ambitious goal to identify and characterize in a comprehensive way the transcription factors and chromatin regulators which initiate and regulate ZGA in a parental specific manner in early mouse embryos. We will utilize novel and highly sensitive genomic approaches to measure nascent transcription and determine open and modified chromatin landscapes in oocytes and early embryos, wild-type and conditionally deficient for major epigenetic modifiers. We will apply computational approaches to identify candidate TFs and histone modifiers controlling ZGA. We will use molecular and developmental biology approaches, combined with sensitive quantitative live-imaging, to interrogate the function of TFs and their binding sites for ZGA.

We will further investigate the significance of possible paternal inheritance of nucleosomes at CpG islands for gene regulation during ZGA and later development by depleting nucleosomes from mature sperm by using sophisticated conditional deficiency and gain-of-function mouse models. By transferring nuclei of immature spermatid and mature sperm into oocytes, we will interrogate the relevance of nucleosome eviction during spermatogenesis, as a possibly truly epigenetic reprogramming process, for defining embryonic competence.

ERC funding would represent a crucial contribution to dissecting the molecular mechanisms underlying acquisition of totipotency in mouse embryos and may impact on the use of Assisted Reproductive Technologies in human med


year authors and title journal last update
List of publications.
2018 Mark E. Gill, Antoine H. F. M. Peters
Toward human egg-like cells in vitro
published pages: 291-292, ISSN: 0036-8075, DOI: 10.1126/science.aav3479
Science 362/6412 2019-06-03
2019 Maaike Welling, Manuel Alexander Mohr, Aaron Ponti, Lluc Rullan Sabater, Andrea Boni, Yumiko K Kawamura, Prisca Liberali, Antoine HFM Peters, Pawel Pelczar, Periklis Pantazis
Primed Track, high-fidelity lineage tracing in mouse pre-implantation embryos using primed conversion of photoconvertible proteins
published pages: , ISSN: 2050-084X, DOI: 10.7554/elife.44491
eLife 8 2019-06-03

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The information about "TOTIPOTENCY" are provided by the European Opendata Portal: CORDIS opendata.

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