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GENOMIS SIGNED

Illuminating GENome Organization through integrated MIcroscopy and Sequencing

Total Cost €

0

EC-Contrib. €

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Partnership

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 Outcomes and results

 GENOMIS project word cloud

Explore the words cloud of the GENOMIS project. It provides you a very rough idea of what is the project "GENOMIS" about.

environment    nucleus    visualization    biology    fixed    unprecedented    size    deciphering    thousands    explore    resolution    technologies    questions    devised    single    compressed    tools    sequencing    3d    genomic    compaction    gene    organization    framework    gpseq    chromosomal    experiments    dozens    genetic    folding    contemporary    cell    combine    linear    first    structural    magnitude    establishing    embrace    maps    models    combining    nuclear    shapes    microscopy    sequence    dna    functions    profiling    localization    coupled    genome    types    vary    govern    optimally    simultaneous    preserved    human    ensue    expression    radial    principles    structure    powerful    positioning    locations    dictate    smaller    discover    cells    preliminary    interconnected    chromosome    datasets    contact    central    morphology    type    illuminate    amazing    fundamental    loci    obtain    link    analytical    repertoire    packed    parallel    orders    probability    function    meters    visualize    read   

Project "GENOMIS" data sheet

The following table provides information about the project.

Coordinator		 KAROLINSKA INSTITUTET 

Organization address
address: Nobels Vag 5
city: STOCKHOLM
postcode: 17177
website: www.ki.se

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.
 Coordinator Country Sweden [SE]
 Project website http://ifish4u.org/
 Total cost 1˙499˙808 €
 EC max contribution 1˙499˙808 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-STG
 Funding Scheme ERC-STG
 Starting year 2018
 Duration (year-month-day) from 2018-01-01   to  2022-12-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    KAROLINSKA INSTITUTET SE (STOCKHOLM) coordinator 1˙499˙808.00

Map

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Leaflet | Map data © OpenStreetMap contributors, CC-BY-SA, Imagery © Mapbox

 Project objective

In human cells, two meters of DNA sequence are compressed into a nucleus whose linear size is five orders of magnitude smaller. Deciphering how this amazing structural organization is achieved and how DNA functions can ensue in the environment of a cell’s nucleus represent central questions for contemporary biology.

Here, I embrace this challenge by establishing a comprehensive framework of microscopy and sequencing technologies coupled with advanced analytical approaches, aimed at addressing three fundamental highly-interconnected questions: 1) What are the design principles that govern DNA compaction? 2) How does genome structure vary between different cell types as well as among cells of the same type? 3) What is the link between genome structure and function? In preliminary experiments, we have devised a powerful method for Genomic loci Positioning by Sequencing (GPSeq) in fixed cells with optimally preserved nuclear morphology. In parallel, we are developing high-end microscopy tools for simultaneous localization of dozens of genomic locations at high resolution in thousands of single cells.

We will obtain first-ever genome-wide maps of radial positioning of DNA loci in the nucleus, and combine them with available DNA contact probability maps in order to build 3D models of the human genome structure in different cell types. Using microscopy, we will visualize chromosomal shapes at unprecedented resolution, and use these rich datasets to discover general DNA folding principles. Finally, by combining high-resolution chromosome visualization with gene expression profiling in single cells, we will explore the link between DNA structure and function. Our study shall illuminate the design principles that dictate how genetic information is packed and read in the human nucleus, while providing a comprehensive repertoire of tools for studying genome organization.

 Publications

year authors and title journal last update
List of publications.
2019 Eleni Gelali, Gabriele Girelli, Masahiro Matsumoto, Erik Wernersson, Joaquin Custodio, Ana Mota, Maud Schweitzer, Katalin Ferenc, Xinge Li, Reza Mirzazadeh, Federico Agostini, John P. Schell, Fredrik Lanner, Nicola Crosetto, Magda Bienko
iFISH is a publically available resource enabling versatile DNA FISH to study genome architecture
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-019-09616-w 		Nature Communications 10/1 2019-09-26

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The information about "GENOMIS" are provided by the European Opendata Portal: CORDIS opendata.

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