Opendata, web and dolomites
  1. home
  2. trasparenza
  3. open h2020
  4. genomis

GENOMIS SIGNED

Illuminating GENome Organization through integrated MIcroscopy and Sequencing

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0
 Outcomes and results

 GENOMIS project word cloud

Explore the words cloud of the GENOMIS project. It provides you a very rough idea of what is the project "GENOMIS" about.

loci    dictate    profiling    human    radial    devised    sequencing    microscopy    datasets    framework    principles    single    structural    positioning    nucleus    simultaneous    tools    gene    optimally    analytical    obtain    morphology    localization    preserved    linear    explore    expression    functions    fixed    locations    chromosome    structure    ensue    powerful    smaller    shapes    chromosomal    coupled    experiments    dna    3d    link    read    technologies    central    compressed    fundamental    genome    models    organization    visualization    folding    nuclear    function    meters    repertoire    amazing    parallel    combine    biology    contemporary    discover    cells    environment    gpseq    visualize    packed    magnitude    establishing    first    sequence    illuminate    probability    genomic    unprecedented    genetic    embrace    size    combining    cell    vary    resolution    questions    type    interconnected    compaction    govern    types    dozens    maps    contact    orders    preliminary    thousands    deciphering   

Project "GENOMIS" data sheet

The following table provides information about the project.

Coordinator		 KAROLINSKA INSTITUTET 

Organization address
address: Nobels Vag 5
city: STOCKHOLM
postcode: 17177
website: www.ki.se

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.
 Coordinator Country Sweden [SE]
 Project website http://ifish4u.org/
 Total cost 1˙499˙808 €
 EC max contribution 1˙499˙808 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-STG
 Funding Scheme ERC-STG
 Starting year 2018
 Duration (year-month-day) from 2018-01-01   to  2022-12-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    KAROLINSKA INSTITUTET SE (STOCKHOLM) coordinator 1˙499˙808.00

Map

 Project objective

In human cells, two meters of DNA sequence are compressed into a nucleus whose linear size is five orders of magnitude smaller. Deciphering how this amazing structural organization is achieved and how DNA functions can ensue in the environment of a cell’s nucleus represent central questions for contemporary biology.

Here, I embrace this challenge by establishing a comprehensive framework of microscopy and sequencing technologies coupled with advanced analytical approaches, aimed at addressing three fundamental highly-interconnected questions: 1) What are the design principles that govern DNA compaction? 2) How does genome structure vary between different cell types as well as among cells of the same type? 3) What is the link between genome structure and function? In preliminary experiments, we have devised a powerful method for Genomic loci Positioning by Sequencing (GPSeq) in fixed cells with optimally preserved nuclear morphology. In parallel, we are developing high-end microscopy tools for simultaneous localization of dozens of genomic locations at high resolution in thousands of single cells.

We will obtain first-ever genome-wide maps of radial positioning of DNA loci in the nucleus, and combine them with available DNA contact probability maps in order to build 3D models of the human genome structure in different cell types. Using microscopy, we will visualize chromosomal shapes at unprecedented resolution, and use these rich datasets to discover general DNA folding principles. Finally, by combining high-resolution chromosome visualization with gene expression profiling in single cells, we will explore the link between DNA structure and function. Our study shall illuminate the design principles that dictate how genetic information is packed and read in the human nucleus, while providing a comprehensive repertoire of tools for studying genome organization.

 Publications

year authors and title journal last update
List of publications.
2019 Eleni Gelali, Gabriele Girelli, Masahiro Matsumoto, Erik Wernersson, Joaquin Custodio, Ana Mota, Maud Schweitzer, Katalin Ferenc, Xinge Li, Reza Mirzazadeh, Federico Agostini, John P. Schell, Fredrik Lanner, Nicola Crosetto, Magda Bienko
iFISH is a publically available resource enabling versatile DNA FISH to study genome architecture
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-019-09616-w 		Nature Communications 10/1 2019-09-26

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "GENOMIS" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "GENOMIS" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

ERC VP CSA (2018)

Support to the Vice-Presidents of the ERC Scientific Council 2018

Read More  

CellProbe (2019)

CellProbe: Microfluidic probe for simultaneous tagging and extraction of single cells

Read More  

CELPRED (2020)

Circuit elements of the cortical circuit for predictive processing

Read More