GENOMIS SIGNED
Illuminating GENome Organization through integrated MIcroscopy and Sequencing
Total Cost €
0EC-Contrib. €
0Partnership
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0GENOMIS project word cloud
Explore the words cloud of the GENOMIS project. It provides you a very rough idea of what is the project "GENOMIS" about.
Project "GENOMIS" data sheet
The following table provides information about the project.
| Coordinator |
KAROLINSKA INSTITUTET
Organization address contact info |
| Coordinator Country | Sweden [SE] |
| Project website | http://ifish4u.org/ |
| Total cost | 1˙499˙808 € |
| EC max contribution | 1˙499˙808 € (100%) |
| Programme |
1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC)) |
| Code Call | ERC-2016-STG |
| Funding Scheme | ERC-STG |
| Starting year | 2018 |
| Duration (year-month-day) | from 2018-01-01 to 2022-12-31 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | KAROLINSKA INSTITUTET | SE (STOCKHOLM) | coordinator | 1˙499˙808.00 |
Map
Project objective
In human cells, two meters of DNA sequence are compressed into a nucleus whose linear size is five orders of magnitude smaller. Deciphering how this amazing structural organization is achieved and how DNA functions can ensue in the environment of a cell’s nucleus represent central questions for contemporary biology.
Here, I embrace this challenge by establishing a comprehensive framework of microscopy and sequencing technologies coupled with advanced analytical approaches, aimed at addressing three fundamental highly-interconnected questions: 1) What are the design principles that govern DNA compaction? 2) How does genome structure vary between different cell types as well as among cells of the same type? 3) What is the link between genome structure and function? In preliminary experiments, we have devised a powerful method for Genomic loci Positioning by Sequencing (GPSeq) in fixed cells with optimally preserved nuclear morphology. In parallel, we are developing high-end microscopy tools for simultaneous localization of dozens of genomic locations at high resolution in thousands of single cells.
We will obtain first-ever genome-wide maps of radial positioning of DNA loci in the nucleus, and combine them with available DNA contact probability maps in order to build 3D models of the human genome structure in different cell types. Using microscopy, we will visualize chromosomal shapes at unprecedented resolution, and use these rich datasets to discover general DNA folding principles. Finally, by combining high-resolution chromosome visualization with gene expression profiling in single cells, we will explore the link between DNA structure and function. Our study shall illuminate the design principles that dictate how genetic information is packed and read in the human nucleus, while providing a comprehensive repertoire of tools for studying genome organization.
Publications
| year | authors and title | journal | last update |
|---|---|---|---|
| 2019 |
Eleni Gelali, Gabriele Girelli, Masahiro Matsumoto, Erik Wernersson, Joaquin Custodio, Ana Mota, Maud Schweitzer, Katalin Ferenc, Xinge Li, Reza Mirzazadeh, Federico Agostini, John P. Schell, Fredrik Lanner, Nicola Crosetto, Magda Bienko iFISH is a publically available resource enabling versatile DNA FISH to study genome architecture published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-019-09616-w |
Nature Communications 10/1 | 2019-09-26 |
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