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GENOMIS SIGNED

Illuminating GENome Organization through integrated MIcroscopy and Sequencing

Total Cost €

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EC-Contrib. €

0

Partnership

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 GENOMIS project word cloud

Explore the words cloud of the GENOMIS project. It provides you a very rough idea of what is the project "GENOMIS" about.

establishing    radial    microscopy    contemporary    functions    biology    environment    folding    nuclear    dna    preliminary    linear    loci    central    structure    explore    coupled    type    human    illuminate    govern    powerful    meters    analytical    sequencing    preserved    3d    resolution    types    parallel    profiling    fixed    packed    structural    shapes    combine    gene    framework    magnitude    dictate    nucleus    positioning    compressed    repertoire    compaction    amazing    discover    questions    thousands    embrace    visualization    read    datasets    contact    tools    experiments    unprecedented    cells    size    genomic    sequence    locations    vary    expression    probability    chromosomal    devised    localization    smaller    gpseq    models    orders    dozens    interconnected    fundamental    maps    genetic    first    obtain    cell    deciphering    single    chromosome    function    morphology    simultaneous    technologies    visualize    principles    genome    link    combining    organization    optimally    ensue   

Project "GENOMIS" data sheet

The following table provides information about the project.

Coordinator
KAROLINSKA INSTITUTET 

Organization address
address: Nobels Vag 5
city: STOCKHOLM
postcode: 17177
website: www.ki.se

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Sweden [SE]
 Project website http://ifish4u.org/
 Total cost 1˙499˙808 €
 EC max contribution 1˙499˙808 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-STG
 Funding Scheme ERC-STG
 Starting year 2018
 Duration (year-month-day) from 2018-01-01   to  2022-12-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    KAROLINSKA INSTITUTET SE (STOCKHOLM) coordinator 1˙499˙808.00

Map

 Project objective

In human cells, two meters of DNA sequence are compressed into a nucleus whose linear size is five orders of magnitude smaller. Deciphering how this amazing structural organization is achieved and how DNA functions can ensue in the environment of a cell’s nucleus represent central questions for contemporary biology.

Here, I embrace this challenge by establishing a comprehensive framework of microscopy and sequencing technologies coupled with advanced analytical approaches, aimed at addressing three fundamental highly-interconnected questions: 1) What are the design principles that govern DNA compaction? 2) How does genome structure vary between different cell types as well as among cells of the same type? 3) What is the link between genome structure and function? In preliminary experiments, we have devised a powerful method for Genomic loci Positioning by Sequencing (GPSeq) in fixed cells with optimally preserved nuclear morphology. In parallel, we are developing high-end microscopy tools for simultaneous localization of dozens of genomic locations at high resolution in thousands of single cells.

We will obtain first-ever genome-wide maps of radial positioning of DNA loci in the nucleus, and combine them with available DNA contact probability maps in order to build 3D models of the human genome structure in different cell types. Using microscopy, we will visualize chromosomal shapes at unprecedented resolution, and use these rich datasets to discover general DNA folding principles. Finally, by combining high-resolution chromosome visualization with gene expression profiling in single cells, we will explore the link between DNA structure and function. Our study shall illuminate the design principles that dictate how genetic information is packed and read in the human nucleus, while providing a comprehensive repertoire of tools for studying genome organization.

 Publications

year authors and title journal last update
List of publications.
2019 Eleni Gelali, Gabriele Girelli, Masahiro Matsumoto, Erik Wernersson, Joaquin Custodio, Ana Mota, Maud Schweitzer, Katalin Ferenc, Xinge Li, Reza Mirzazadeh, Federico Agostini, John P. Schell, Fredrik Lanner, Nicola Crosetto, Magda Bienko
iFISH is a publically available resource enabling versatile DNA FISH to study genome architecture
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-019-09616-w
Nature Communications 10/1 2019-09-26

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