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Stress Imaging SIGNED

Nanoscale Stress Imaging with Imperfect Diamonds

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Project "Stress Imaging" data sheet

The following table provides information about the project.

Coordinator
ACADEMISCH ZIEKENHUIS GRONINGEN 

Organization address
address: HANZEPLEIN 1
city: GRONINGEN
postcode: 9713 GZ
website: www.umcg.nl

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Netherlands [NL]
 Total cost 1˙454˙713 €
 EC max contribution 1˙454˙713 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-STG
 Funding Scheme ERC-STG
 Starting year 2017
 Duration (year-month-day) from 2017-03-01   to  2022-02-28

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    ACADEMISCH ZIEKENHUIS GRONINGEN NL (GRONINGEN) coordinator 1˙454˙713.00

Map

 Project objective

My goal is to optically detect the magnetic resonance of free radicals/ROS inside cells. Radicals are suspected to play a crucial role in numerous pathogenic conditions including diseases responsible for most deaths worldwide (as arteriosclerosis, cancer, immune responses to pathogens). They are also involved in many processes in healthy cells as mitochondrial metabolism or aging of cells and part of the working mechanism of many drugs. Despite their relevance relatively little is known about where and when radicals are built, how they work or which ones play a role. Their short lifetime and reactivity poses a problem for many state of the art methods. Thus they are often a bottleneck in understanding stress responses. My goal is to develop a method, which can detect their magnetic resonance in the nanoscale. The method is based on a fluorescent defect in diamond, which changes its optical properties based on its magnetic surrounding. While this technique has been able to detect even the faint signal of a single electron spin, this technique is entirely new to biological fields. We can localize where, when and how much of a certain radical is generated with nm resolution. This is impossible with the current state of the art. Furthermore, since we obtain spectra we can also differentiate radicals to some extent. I am proposing to investigate two systems: 1) the involvement of radicals in the aging of yeast cells 2) the response of macrophages to stress. In the first project I will test the so-called free radical theory, which states that organisms age because cells accumulate free radical damage over time. In the second project I will answer the question how a macrophage reacts to the impact of a pathogen or a drug. Outcomes of this project would enable us to increase our understanding on how stress responses work on a molecular level. This will open up new possibilities to assess if and how drugs are working or how and why certain pathogens are worse than others.

 Publications

year authors and title journal last update
List of publications.
2020 Aryan Morita, Thamir Hamoh, Felipe P. Perona Martinez, Mayeul Chipaux, Alina Sigaeva, Charles Mignon, Kiran J. van der Laan, Axel Hochstetter, Romana Schirhagl
The Fate of Lipid-Coated and Uncoated Fluorescent Nanodiamonds during Cell Division in Yeast
published pages: 516, ISSN: 2079-4991, DOI: 10.3390/nano10030516
Nanomaterials 10/3 2020-04-24
2020 Kiran J. van der Laan, Aryan Morita, Felipe P. Perona-Martinez, Romana Schirhagl
Evaluation of the Oxidative Stress Response of Aging Yeast Cells in Response to Internalization of Fluorescent Nanodiamond Biosensors
published pages: 372, ISSN: 2079-4991, DOI: 10.3390/nano10020372
Nanomaterials 10/2 2020-04-24
2019 Alina Sigaeva, Yori Ong, Viraj G. Damle, Aryan Morita, Kiran J. van der Laan, Romana Schirhagl
Optical Detection of Intracellular Quantities Using Nanoscale Technologies
published pages: 1739-1749, ISSN: 0001-4842, DOI: 10.1021/acs.accounts.9b00102
Accounts of Chemical Research 52/7 2019-11-26
2018 Alina Sigaeva, Thamir Hamoh, Felipe Perona, Romana Schirhagl
P-141 - Fluorescent nanodiamonds: potential free radical detectors in live cells
published pages: , ISSN: , DOI:
Free Radical Biology and Medicine 2019-05-14
2018 Tingting Zheng Felipe Perona Martínez Ingeborg Maria Storm Wolf Rombouts Joris Sprakel Renko de Vries Romana Schirhagl
De Novo Designed Proteins for Colloidal Stabilization and Improvement of Cellular Uptake
published pages: , ISSN: , DOI:
Biophysical Journal 2019-05-14
2016 Simon R. Hemelaar, Andreas Nagl, François Bigot, Melissa M. Rodríguez-García, Marcel P. de Vries, Mayeul Chipaux, Romana Schirhagl
The interaction of fluorescent nanodiamond probes with cellular media
published pages: , ISSN: 1436-5073, DOI:
Microchimica Acta 2019-05-14
2018 Simon Hemelaar, Babujhi Saspaanithy, Severin L’Hommelet, Felipe Perona Martinez, Kiran van der Laan, Romana Schirhagl
The Response of HeLa Cells to Fluorescent NanoDiamond Uptake
published pages: 355, ISSN: 1424-8220, DOI: 10.3390/s18020355
Sensors 18/2 2019-05-14

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