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SMSC SIGNED

Single-Molecule Studies on Condensin

Total Cost €

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EC-Contrib. €

0

Partnership

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 SMSC project word cloud

Explore the words cloud of the SMSC project. It provides you a very rough idea of what is the project "SMSC" about.

mitosis    magnetic    meters    condensin    fluorescent    structure    biophysics    enters    spatiotemporal    biochemistry    genome    demands    kleisin    unknown    1nm    mechanism    few    compact    condensation    correlate    compacted    protein    cees    interactions    dimers    molecule    dna    of    synergetic    hottest    physiological    hydrolysis    daughter    formed    understand    hs    rate    single    speed    shapes    copy    entire    for    dekker    orthogonal    molecular    ryu    division    prof    innovative    afm    lab    kyung    images    volume    clues    binding    micrometer    fluorescence    time    cell    50ms    meet    imaging    cooperative    exactly    resolution    domains    embraces    je    size    collectively    experts    video    leader    exits    record    replicated    world    segregated    chromosome    heat    cells    gets    chromosomes    laboratory    proposes    combined    ring    conformational    smc    distributed    interdisciplinary    tweezers    techniques    condenses    dr    small    effort    reveal    technique    microscopy    atp   

Project "SMSC" data sheet

The following table provides information about the project.

Coordinator
TECHNISCHE UNIVERSITEIT DELFT 

Organization address
address: STEVINWEG 1
city: DELFT
postcode: 2628 CN
website: www.tudelft.nl

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Netherlands [NL]
 Project website https://ceesdekkerlab.nl
 Total cost 177˙598 €
 EC max contribution 177˙598 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-03-01   to  2019-02-28

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    TECHNISCHE UNIVERSITEIT DELFT NL (DELFT) coordinator 177˙598.00

Map

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 Project objective

For division of each of our cells, 2 meters of DNA should be replicated, compacted into a few-micrometer size chromosomes that are segregated and distributed via mitosis, so that each daughter cell gets exactly one copy. How this large DNA genome is well organized into such a small volume is one of the hottest issues for the understanding of chromosomes. Condensin, a ring-like structure formed by SMC dimers, kleisin, and two heat domains, embraces DNA and condenses the DNA using ATP hydrolysis. However, the molecular mechanism on how conformational changes of condensin compact DNA is still unknown. Here, the applicant Dr. Je-Kyung Ryu proposes to use two orthogonal single-molecule approaches, “high-speed AFM (HS AFM)” and “single-molecule fluorescent magnetic tweezers” to reveal the molecular mechanism of condensin. HS AFM imaging is a unique technique to record video-rate real-time images for entire structure of a protein in physiological condition with high spatiotemporal resolution (~1nm, ~50ms). In addition, using single-molecule fluorescence microscopy combined with magnetic tweezers, the applicant can understand how the cooperative binding of condensin to DNA correlate with DNA condensation. These two approaches will not only provide most important clues to understand the mechanism of condensin how DNA enters and exits the condensin ring and how condensin shapes chromosome but also include technical advances in single-molecule techniques. This work will be carried out in the laboratory of Prof. Cees Dekker, who is a world leader in HS AFM and single-molecule biophysics of DNA-protein interactions. The applicant’s experts in single-molecule biophysics, fluorescence microscopy, and biochemistry will make synergetic effects with the resources of the Dekker lab to collectively meet the interdisciplinary demands of the innovative effort.

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