Opendata, web and dolomites

SMSC SIGNED

Single-Molecule Studies on Condensin

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 SMSC project word cloud

Explore the words cloud of the SMSC project. It provides you a very rough idea of what is the project "SMSC" about.

effort    cees    fluorescent    condensin    biochemistry    reveal    mitosis    techniques    domains    hottest    shapes    innovative    enters    tweezers    chromosome    exits    genome    segregated    copy    synergetic    distributed    meters    interdisciplinary    je    entire    cells    structure    compacted    condenses    technique    embraces    molecule    proposes    cooperative    for    magnetic    world    size    collectively    afm    micrometer    demands    hydrolysis    speed    rate    daughter    exactly    clues    images    orthogonal    leader    protein    spatiotemporal    condensation    hs    physiological    ryu    resolution    1nm    dekker    of    small    dimers    division    cell    experts    gets    time    record    ring    chromosomes    compact    atp    conformational    microscopy    single    imaging    fluorescence    heat    mechanism    50ms    laboratory    smc    understand    dr    unknown    correlate    few    kleisin    kyung    meet    formed    video    biophysics    molecular    prof    combined    replicated    dna    lab    volume    binding    interactions   

Project "SMSC" data sheet

The following table provides information about the project.

Coordinator
TECHNISCHE UNIVERSITEIT DELFT 

Organization address
address: STEVINWEG 1
city: DELFT
postcode: 2628 CN
website: www.tudelft.nl

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Netherlands [NL]
 Project website https://ceesdekkerlab.nl
 Total cost 177˙598 €
 EC max contribution 177˙598 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-03-01   to  2019-02-28

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    TECHNISCHE UNIVERSITEIT DELFT NL (DELFT) coordinator 177˙598.00

Map

 Project objective

For division of each of our cells, 2 meters of DNA should be replicated, compacted into a few-micrometer size chromosomes that are segregated and distributed via mitosis, so that each daughter cell gets exactly one copy. How this large DNA genome is well organized into such a small volume is one of the hottest issues for the understanding of chromosomes. Condensin, a ring-like structure formed by SMC dimers, kleisin, and two heat domains, embraces DNA and condenses the DNA using ATP hydrolysis. However, the molecular mechanism on how conformational changes of condensin compact DNA is still unknown. Here, the applicant Dr. Je-Kyung Ryu proposes to use two orthogonal single-molecule approaches, “high-speed AFM (HS AFM)” and “single-molecule fluorescent magnetic tweezers” to reveal the molecular mechanism of condensin. HS AFM imaging is a unique technique to record video-rate real-time images for entire structure of a protein in physiological condition with high spatiotemporal resolution (~1nm, ~50ms). In addition, using single-molecule fluorescence microscopy combined with magnetic tweezers, the applicant can understand how the cooperative binding of condensin to DNA correlate with DNA condensation. These two approaches will not only provide most important clues to understand the mechanism of condensin how DNA enters and exits the condensin ring and how condensin shapes chromosome but also include technical advances in single-molecule techniques. This work will be carried out in the laboratory of Prof. Cees Dekker, who is a world leader in HS AFM and single-molecule biophysics of DNA-protein interactions. The applicant’s experts in single-molecule biophysics, fluorescence microscopy, and biochemistry will make synergetic effects with the resources of the Dekker lab to collectively meet the interdisciplinary demands of the innovative effort.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "SMSC" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "SMSC" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

Widow Spider Mating (2020)

Immature mating as a novel tactic of an invasive widow spider

Read More  

TARGET SLEEP (2020)

Boosting motor learning through sleep and targeted memory reactivation in ageing and Parkinson’s disease

Read More  

MY MITOCOMPLEX (2021)

Functional relevance of mitochondrial supercomplex assembly in myeloid cells

Read More