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SMSC SIGNED

Single-Molecule Studies on Condensin

Total Cost €

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EC-Contrib. €

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Partnership

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 SMSC project word cloud

Explore the words cloud of the SMSC project. It provides you a very rough idea of what is the project "SMSC" about.

kleisin    collectively    interactions    interdisciplinary    imaging    ring    dr    biophysics    innovative    cell    magnetic    cooperative    reveal    distributed    exactly    gets    shapes    physiological    protein    cells    spatiotemporal    binding    mitosis    synergetic    few    small    proposes    dekker    understand    formed    enters    condensation    volume    orthogonal    microscopy    structure    tweezers    prof    demands    division    meters    condensin    atp    video    segregated    of    world    experts    entire    single    size    mechanism    kyung    fluorescence    chromosomes    micrometer    rate    heat    technique    for    1nm    record    combined    time    exits    molecule    unknown    embraces    daughter    dna    50ms    afm    effort    molecular    ryu    hydrolysis    conformational    compact    leader    je    fluorescent    images    techniques    correlate    laboratory    meet    dimers    genome    lab    compacted    speed    biochemistry    cees    domains    resolution    hottest    clues    chromosome    condenses    copy    smc    replicated    hs   

Project "SMSC" data sheet

The following table provides information about the project.

Coordinator
TECHNISCHE UNIVERSITEIT DELFT 

Organization address
address: STEVINWEG 1
city: DELFT
postcode: 2628 CN
website: www.tudelft.nl

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Netherlands [NL]
 Project website https://ceesdekkerlab.nl
 Total cost 177˙598 €
 EC max contribution 177˙598 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-03-01   to  2019-02-28

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    TECHNISCHE UNIVERSITEIT DELFT NL (DELFT) coordinator 177˙598.00

Map

 Project objective

For division of each of our cells, 2 meters of DNA should be replicated, compacted into a few-micrometer size chromosomes that are segregated and distributed via mitosis, so that each daughter cell gets exactly one copy. How this large DNA genome is well organized into such a small volume is one of the hottest issues for the understanding of chromosomes. Condensin, a ring-like structure formed by SMC dimers, kleisin, and two heat domains, embraces DNA and condenses the DNA using ATP hydrolysis. However, the molecular mechanism on how conformational changes of condensin compact DNA is still unknown. Here, the applicant Dr. Je-Kyung Ryu proposes to use two orthogonal single-molecule approaches, “high-speed AFM (HS AFM)” and “single-molecule fluorescent magnetic tweezers” to reveal the molecular mechanism of condensin. HS AFM imaging is a unique technique to record video-rate real-time images for entire structure of a protein in physiological condition with high spatiotemporal resolution (~1nm, ~50ms). In addition, using single-molecule fluorescence microscopy combined with magnetic tweezers, the applicant can understand how the cooperative binding of condensin to DNA correlate with DNA condensation. These two approaches will not only provide most important clues to understand the mechanism of condensin how DNA enters and exits the condensin ring and how condensin shapes chromosome but also include technical advances in single-molecule techniques. This work will be carried out in the laboratory of Prof. Cees Dekker, who is a world leader in HS AFM and single-molecule biophysics of DNA-protein interactions. The applicant’s experts in single-molecule biophysics, fluorescence microscopy, and biochemistry will make synergetic effects with the resources of the Dekker lab to collectively meet the interdisciplinary demands of the innovative effort.

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The information about "SMSC" are provided by the European Opendata Portal: CORDIS opendata.

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