Opendata, web and dolomites


Genetic landscape of the homology search

Total Cost €


EC-Contrib. €






 RecPAIR project word cloud

Explore the words cloud of the RecPAIR project. It provides you a very rough idea of what is the project "RecPAIR" about.

life    dcas9    lesions    quality    genes    microfluidic    conclude    hr    central    dna    crispri    combination    library    defects    faithful    directed    screen    elusive    gene    molecular    refined    characterise    vulnerable    map    sequencing    ageing    external    integrity    phenotype    broken    phenotyping    strands    recombination    dsbs    recover    tested    situ    uses    double    dynamics    linked    dsb    pooled    induction    breaks    characterised    cell    strains    strand    screening    characterisation    homology    vivo    functional    cancer    landscape    repair    first    genetic    coupled    probability    incorrect    sources    internal    model    perhaps    genotyping    position    dumpling    single    chromosome    coli    movements    homologous    genome    fluorescent    template    microscopy    replicated    monitored    center    throughput    pairs    drives    threatening    create    interrupted    search    lies    localises    crispr    limit    mutagenesis    damage    phenotypic    duplex   

Project "RecPAIR" data sheet

The following table provides information about the project.


Organization address
postcode: 751 05

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Sweden [SE]
 Total cost 191˙852 €
 EC max contribution 191˙852 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2019
 Duration (year-month-day) from 2019-06-08   to  2021-06-07


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UPPSALA UNIVERSITET SE (UPPSALA) coordinator 191˙852.00


 Project objective

The integrity of genetic information is central to life, yet the DNA is vulnerable to damage from internal and external sources. Incorrect repair of DNA damage drives mutagenesis, loss of genetic information, ageing, and cancer. Double strand DNA breaks (DSBs) are perhaps the most threatening DNA lesions, where the integrity of both strands of the DNA duplex is interrupted at the same position. In E. coli, faithful repair of DSBs is possible only through the homologous recombination (HR) pathway which uses replicated chromosome as a template to recover the information. At the center of HR lies an elusive search process, during which broken strand localises and pairs with the repair template.

I will use a combination of CRISPR/dCas9 screening and in-situ genotyping of pooled library of strains to characterise the genetic landscape controlling the homology search. First, I will develop a low probability DSB induction method, to limit the DSB-formation to only a single chromosome per cell. Next, I will design and implement a whole-genome CRISPRi screen coupled to high-throughput sequencing and map the genes involved specifically in the homology directed repair of DSBs. The knowledge of the recombination-specific genes will allow to create a refined, high-quality phenotypic screen. In this screen the whole chromosome dynamics will be monitored and defects in the DNA movements will be characterised for each tested target with a microfluidic-based fluorescent microscopy. Each phenotype will be linked to a specific gene using the state-of-the-art in-situ phenotyping approach called DuMPLING. The functional characterisation of recombination genes will allow to conclude a molecular model of the search process in vivo.

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The information about "RECPAIR" are provided by the European Opendata Portal: CORDIS opendata.

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