Explore the words cloud of the TF-bind Determinants project. It provides you a very rough idea of what is the project "TF-bind Determinants" about.
The following table provides information about the project.
FRIEDRICH MIESCHER INSTITUTE FOR BIOMEDICAL RESEARCH FONDATION
|Coordinator Country||Switzerland [CH]|
|Total cost||187˙419 €|
|EC max contribution||187˙419 € (100%)|
1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
|Duration (year-month-day)||from 2017-04-01 to 2019-03-31|
Take a look of project's partnership.
|1||FRIEDRICH MIESCHER INSTITUTE FOR BIOMEDICAL RESEARCH FONDATION||CH (BASEL)||coordinator||187˙419.00|
Transcription factors (TFs) read cis-regulatory information encoded in the genome and by doing so, they establish gene expression patterns. However, TFs must compete with structural chromatin proteins such as nucleosomes for access to DNA, and this may partially explain why only a fraction of their sequence motifs in the genome are actually bound. It is unclear what features discriminate used from unused sites with similar motifs, in vivo, though it is likely that sensitivity of TFs to chromatin is critical for normal gene expression. For example, some TFs have reduced chromatin sensitivity, termed pioneer-factors, and are able to drive differentiation; even these bind a minority of cognate motifs in the genome. Such factors may nevertheless modify chromatin and expose motifs for TFs with higher sensitivity. Alternatively, chromatin proteins and modifications may be intrinsically directed by unidentified DNA sequence features. Though little is known about determines of TF-chromatin sensitivity, it is likely a function of DNA affinity and the ability to recruit chromatin-modifying activity. The complexity of chromatin/TF interactions necessitates a reductionist approach. The objectives of this project is to gain mechanistic understanding of chromatin-sensitivity and will use established methods to measure binding of ectopic TFs as a function TF properties and chromatin components. I will express exogenous TFs in mammalian cells and compare in vivo binding to in vitro DNA affinity and analyse their chromatin sensitivity (WP1). This setup enables me to modify and profile chromatin before and after expression, which will test the contribution these factors have on TF binding (WP2). Finally, I will test if manipulating the chromatin-modifying activity of an ectopic TF affects binding (WP3). These results will provide mechanistic insight into TF sensitivity to chromatin and should reveal general principles of binding hierarchies and the logic of cis-regulatory regions.
|year||authors and title||journal||last update|
Dominik Hartl, Arnaud R. Krebs, Ralph S. Grand, Tuncay Baubec, Luke Isbel, Christiane Wirbelauer, Lukas Burger, Dirk SchÃ¼beler
CG dinucleotides enhance promoter activity independent of DNA methylation
published pages: 554-563, ISSN: 1088-9051, DOI: 10.1101/gr.241653.118
|Genome Research 29/4||2019-07-18|
Are you the coordinator (or a participant) of this project? Plaese send me more information about the "TF-BIND DETERMINANTS" project.
For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.
Send me an email (firstname.lastname@example.org) and I put them in your project's page as son as possible.
Thanks. And then put a link of this page into your project's website.
The information about "TF-BIND DETERMINANTS" are provided by the European Opendata Portal: CORDIS opendata.
A self-esteem process framework of the transition to workRead More
use of multiscale modElling to Minimize coke ProducTion during the methanol-to- HYdrocarbon processRead More
Programmable NanoRobotics for Controlled Manipulation of Molecular CargoesRead More