Opendata, web and dolomites

EPICODE SIGNED

Programmable Readers, Writers, and Erasers of the Epigenetic Cytosine Code

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 EPICODE project word cloud

Explore the words cloud of the EPICODE project. It provides you a very rough idea of what is the project "EPICODE" about.

modules    cac    photoactivatable    nucleobase    acids    de    molecules    effectors    broadly    fusions    nucleobases    programmable    selectivity    chromatin    recognizes    imprinting    single    base    instructive    synthesis    contains    epigenetics    genomic    consist    typing    revolutionary    vitro    hmc    biological    reading    decoded    profiling    molecular    scaffold    manner    perturbation    encode    cancer    regulatory    methodology    mc    contrast    class    tale    reported    analogs    synthesized    writing    sciences    phenotype    human    cells    regulation    biologically    spatiotemporal    simplicity    fc    activator    central    detection    transcription    pairing    diagnosis    insights    concatenated    erasing    canonical    whereas    dynamic    identity    watson    recognition    shapes    enabled    vivo    novo    biomarker    editing    crick    cell    trigger    applicable    toolbox    expanded    tales    types    epigenetic    rnas    resolution    proteins    engineer    therapy    employ    regulate    dna    loci    universality    nucleic    first    exist   

Project "EPICODE" data sheet

The following table provides information about the project.

Coordinator
TECHNISCHE UNIVERSITAT DORTMUND 

Organization address
address: AUGUST SCHMIDT STRASSE 4
city: DORTMUND
postcode: 44227
website: www.tu-dortmund.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 1˙979˙679 €
 EC max contribution 1˙979˙679 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-COG
 Funding Scheme ERC-COG
 Starting year 2017
 Duration (year-month-day) from 2017-11-01   to  2022-10-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    TECHNISCHE UNIVERSITAT DORTMUND DE (DORTMUND) coordinator 1˙979˙679.00

Map

 Project objective

Human DNA contains two types of biologically instructive information: the canonical nucleobases A, G, T, C, and the epigenetic nucleobases mC, hmC, fC, and caC. Canonical nucleobases encode the identity of all RNAs and proteins that are synthesized by a cell, whereas epigenetic nucleobases regulate this synthesis. This regulation shapes the phenotype of cells, and its perturbation is a key trigger of cancer. Canonical nucleobases can be decoded in a programmable manner by nucleic acids and their analogs via Watson-Crick-base pairing, and the simplicity of this recognition has enabled revolutionary developments in the biological sciences. In contrast, comparable developments in epigenetics have not yet been possible, since a molecular scaffold with programmable recognition of epigenetic nucleobases does not exist. We will establish the first class of molecules capable of the expanded programmable recognition of both canonical and epigenetic DNA nucleobases in vitro and in vivo. This is based on transcription-activator-like effectors (TALEs) that consist of four types of concatenated modules, each of which recognizes a canonical nucleobase. We have recently reported the detection of single epigenetic nucleobases by TALEs. In this project, we will 1. engineer a toolbox of TALE modules with selectivity for C, mC, hmC, fC, and caC, 2. employ them for TALE-based in vitro typing and profiling (reading) of cancer biomarker mC/hmC, and 3. design photoactivatable TALE-fusions that enable the writing and erasing of mC at user-defined genomic loci in vivo with spatiotemporal resolution. This will provide the first insights into the dynamic effects of de novo editing on chromatin regulation, and enables the imprinting of regulatory states. Given the central role of epigenetic nucleobases in cancer and the universality of our approach, this project will provide enabling and broadly applicable methodology for cancer epigenetics research, diagnosis and therapy.

 Publications

year authors and title journal last update
List of publications.
2018 S. Maurer, B. Buchmuller, C. Ehrt, J. Jasper, O. Koch and D. Summerer*
Overcoming conservation in TALE-DNA interactions: A minimal repeat scaffold enables selective rerognition of an oxidized 5-methylcytosine
published pages: , ISSN: 2041-6520, DOI:
Chem. Sci. 2019-06-27
2019 T. Braun, M. Drescher* and D. Summerer*
Expanding the genetic code for site-directed spin-labeling
published pages: , ISSN: 1422-0067, DOI:
Int. J. Mol. Sci. 2019-06-27
2018 H. Neumann*, P. Neumann-Staubitz, A. Witte and D. Summerer*
Epigenetic chromatin modification by amber suppression technology
published pages: , ISSN: 1367-5931, DOI:
Curr. Opin. Chem. Biol. 2019-06-27
2019 M. Giess, A. Munoz-Lopez, B. Buchmuller, G. Kubik and D. Summerer*
Programmable Protein-DNA Crosslinking for the Direct Capture and Quantification of 5-Formylcytosine
published pages: , ISSN: 0002-7863, DOI:
J. Am. Chem. Soc. 2019-06-27

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "EPICODE" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "EPICODE" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

HBPTC (2019)

Hydrogen Bonding Phase Transfer Catalysis

Read More  

NEUTRAMENTH (2018)

A redox-neutral process for the cost-efficient and environmentally friendly production of Menthol

Read More  

HyperBio (2019)

Vis-NIR Hyperspectral imaging for biomaterial quality control

Read More