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Endogenous barcoding for in vivo fate mapping of lineage development in the blood and immune system

Total Cost €


EC-Contrib. €






 BARCODED-CELLTRACING project word cloud

Explore the words cloud of the BARCODED-CELLTRACING project. It provides you a very rough idea of what is the project "BARCODED-CELLTRACING" about.

answers    stem    tested    hematopoiesis    unperturbed    analytical    builds    blood    switched    situ    fluorescent    driver    lineage    realize    progenitor    map    relationships    barcode    manipulations    loxp    hematopoietic    resolve    dna    limited    fates    fate    ultimately    physiological    thousand    stage    until    barcoding    mice    principles    opposed    peripheral    progeny    analyze    rested    tags    resolving    inducible    post    invasive    questions    experiments    developmental    structure    inability    endogenous    power    cell    marked    designed    locus    marker    chose    nodes    cells    mapping    experimental    recombination    mouse    feasibilities    traced    ensemble    mutants    uncovered    dissection    maintains    vivo    fact    single    largely    disruptive    deconvolution    diverse    termed    immune    hsc    genetic    artificial    tissue    routes    transplantation    recombinase    lineages    output    resolution    polylox    cre    isolation    zoo    link    hundred    generate    differences    notably    manner   

Project "BARCODED-CELLTRACING" data sheet

The following table provides information about the project.


Organization address
postcode: 69120

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 2˙500˙000 €
 EC max contribution 2˙500˙000 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-ADG
 Funding Scheme ERC-ADG
 Starting year 2017
 Duration (year-month-day) from 2017-07-01   to  2022-06-30


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

The immune system is a complex ensemble of diverse lineages. Studies on in-vivo-hematopoiesis have until now largely rested on transplantation. More physiological experiments have been limited by the inability to analyze hematopoietic stem (HSC) and progenitor cells in situ without cell isolation and other disruptive manipulations. We have developed mouse mutants in which a fluorescent marker can be switched on in HSC in situ (inducible fate mapping), and traced HSC lineage output under unperturbed conditions in vivo. These experiments uncovered marked differences comparing in situ and post-transplantation hematopoiesis. These new developments raise several important questions, notably on the developmental fates HSC realize in vivo (as opposed to their experimental potential), and on the structure (routes and nodes) of hematopoiesis from HSC to peripheral blood and immune lineages. Answers to these questions (and in fact the deconvolution of any tissue) require the development of non-invasive, high resolution barcoding systems. We have now designed, built and tested a DNA-based barcoding system, termed Polylox, that is based on an artificial recombination locus in which Cre recombinase can generate several hundred thousand genetic tags in mice. We chose the Cre-loxP system to link high resolution barcoding (i.e. the ability to barcode single cells and to fate map their progeny) to the zoo of tissue- or stage-specific, inducible Cre-driver mice. Here, I will present the principles of this endogenous barcoding system, demonstrate its experimental and analytical feasibilities and its power to resolve complex lineages. The work program addresses in a comprehensive manner major open questions on the structure of the hematopoietic system that builds and maintains the immune system. This project ultimately aims at an in depth dissection of unique or common lineage pathways emerging from HSC, and at resolving relationships within cell lineages of the immune system.


year authors and title journal last update
List of publications.
2017 Weike Pei, Thorsten B. Feyerabend, Jens Rössler, Xi Wang, Daniel Postrach, Katrin Busch, Immanuel Rode, Kay Klapproth, Nikolaus Dietlein, Claudia Quedenau, Wei Chen, Sascha Sauer, Stephan Wolf, Thomas Höfer, Hans-Reimer Rodewald
Polylox barcoding reveals haematopoietic stem cell fates realized in vivo
published pages: 456-460, ISSN: 0028-0836, DOI: 10.1038/nature23653
Nature 548/7668 2019-04-18
2018 Thomas Höfer, Hans-Reimer Rodewald
Differentiation-based model of hematopoietic stem cell functions and lineage pathways
published pages: 1106-1113, ISSN: 0006-4971, DOI: 10.1182/blood-2018-03-791517
Blood 132/11 2019-02-12

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