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ELONGAN SIGNED

Gene editing and in vitro approaches to understand conceptus elongation in ungulates

Total Cost €

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EC-Contrib. €

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Partnership

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 ELONGAN project word cloud

Explore the words cloud of the ELONGAN project. It provides you a very rough idea of what is the project "ELONGAN" about.

pursue    interactions    deficiencies    derivatives    conceptuses    fluid    prolonged    editing    profitability    embryos    model    requirement    putatively    ko    failures    frequent    bovine    hatching    human    candidate    unanswered    biology    serve    contrast    genes    experiments    appearance    undergo    play    rationally    crispr    livestock    reducing    conceptus    proliferation    uterus    subsequently    membranes    transferred    rna    vivo    uterine    farming    understand    embryonic    ungulates    proteomics    preimplantation    cell    ablated    vitro    blastocyst    elongation    right    dramatic    maternal    function    questions    termed    embryo    reproductive    metabolomics    flat    mammalian    morphological    strategies    first    females    bypass    purpose    sustain    differentiation    implantation    disc    gene    elucidate    mouse    implant    rodent    gastrulation    species    culture    developmental    migration    recipient    constitute    seq    ungulate    stages    data    perform    extra    hatched   

Project "ELONGAN" data sheet

The following table provides information about the project.

Coordinator
INSTITUTO NACIONAL DE INVESTIGACION Y TECNOLOGIA AGRARIA Y ALIMENTARIA OA MP 

Organization address
address: Carretera de la Coruna Km7.5
city: MADRID
postcode: 28040
website: www.inia.es

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Spain [ES]
 Total cost 1˙480˙880 €
 EC max contribution 1˙480˙880 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-STG
 Funding Scheme ERC-STG
 Starting year 2017
 Duration (year-month-day) from 2017-10-01   to  2022-09-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    INSTITUTO NACIONAL DE INVESTIGACION Y TECNOLOGIA AGRARIA Y ALIMENTARIA OA MP ES (MADRID) coordinator 1˙480˙880.00

Map

 Project objective

In contrast to human or rodent embryos, ungulate embryos do not implant into the uterus right after blastocyst hatching. Before implantation, the hatched ungulate blastocyst must undergo dramatic morphological changes characterized by cell differentiation, proliferation and migration processes leading to the development of extra-embryonic membranes, the appearance of a flat embryonic disc and gastrulation. This prolonged preimplantation development is termed conceptus elongation and deficiencies on this process constitute the most frequent cause of reproductive failures in ungulates, including the 4 most relevant mammalian livestock species in Europe. The purpose of this project is to elucidate the factors involved in conceptus elongation by gene editing and in vitro culture approaches. A first objective will be to identify key genes involved in differentiation processes by RNA-seq analysis of different embryo derivatives from bovine conceptuses at different developmental stages. Subsequently, the function of some of the genes identified as well as others known to play a crucial role in mouse development or putatively involved in embryo-maternal interactions will be assessed. For this aim, bovine embryos in which a candidate gene has been ablated (KO) will be generated by CRISPR and transferred to recipient females to assess in vivo the function of such particular gene on conceptus development. A second set of experiments pursue the development of an in vitro system for conceptus elongation that would bypass the requirement for in vivo experiments. For this aim we will perform metabolomics and proteomics analyses of bovine uterine fluid at different stages and will use these data to rationally develop a culture system able to sustain conceptus development. The knowledge generated by this project will serve to develop strategies to enhance farming profitability by reducing embryonic loss and to understand Developmental Biology questions unanswered by the mouse model.

 Deliverables

List of deliverables.
Data Management Plan Open Research Data Pilot 2019-11-25 12:35:12

Take a look to the deliverables list in detail:  detailed list of ELONGAN deliverables.

 Publications

year authors and title journal last update
List of publications.
2018 Ismael Lamas-Toranzo, Eva Pericuesta, Pablo Bermejo-Álvarez
Mitochondrial and metabolic adjustments during the final phase of follicular development prior to IVM of bovine oocytes
published pages: 156-162, ISSN: 0093-691X, DOI: 10.1016/j.theriogenology.2018.07.007
Theriogenology 119 2019-05-22
2018 Ismael Lamas-Toranzo, Priscila Ramos-Ibeas, Eva Pericuesta, Pablo Bermejo-Álvarez
Directions and applications of CRISPR technology in livestock research
published pages: 292-300, ISSN: 1984-3143, DOI: 10.21451/1984-3143-ar2018-0075
Animal Reproduction 15/3 2019-05-22

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The information about "ELONGAN" are provided by the European Opendata Portal: CORDIS opendata.

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