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ELONGAN SIGNED

Gene editing and in vitro approaches to understand conceptus elongation in ungulates

Total Cost €

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EC-Contrib. €

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Partnership

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 ELONGAN project word cloud

Explore the words cloud of the ELONGAN project. It provides you a very rough idea of what is the project "ELONGAN" about.

species    bypass    uterine    pursue    biology    ungulates    prolonged    migration    function    maternal    females    ungulate    stages    embryos    reproductive    transferred    appearance    editing    rationally    gastrulation    elongation    serve    play    profitability    extra    right    subsequently    farming    bovine    undergo    dramatic    vitro    experiments    culture    seq    constitute    metabolomics    genes    proliferation    data    conceptus    uterus    proteomics    interactions    developmental    blastocyst    cell    crispr    recipient    candidate    rna    conceptuses    implantation    strategies    unanswered    disc    differentiation    hatched    vivo    gene    ko    embryo    understand    model    requirement    first    livestock    putatively    preimplantation    human    termed    mouse    perform    purpose    fluid    mammalian    deficiencies    rodent    contrast    flat    morphological    ablated    frequent    reducing    sustain    hatching    elucidate    membranes    questions    derivatives    implant    failures    embryonic   

Project "ELONGAN" data sheet

The following table provides information about the project.

Coordinator
INSTITUTO NACIONAL DE INVESTIGACION Y TECNOLOGIA AGRARIA Y ALIMENTARIA OA MP 

Organization address
address: Carretera de la Coruna Km7.5
city: MADRID
postcode: 28040
website: www.inia.es

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Spain [ES]
 Total cost 1˙480˙880 €
 EC max contribution 1˙480˙880 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-STG
 Funding Scheme ERC-STG
 Starting year 2017
 Duration (year-month-day) from 2017-10-01   to  2022-09-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    INSTITUTO NACIONAL DE INVESTIGACION Y TECNOLOGIA AGRARIA Y ALIMENTARIA OA MP ES (MADRID) coordinator 1˙480˙880.00

Map

 Project objective

In contrast to human or rodent embryos, ungulate embryos do not implant into the uterus right after blastocyst hatching. Before implantation, the hatched ungulate blastocyst must undergo dramatic morphological changes characterized by cell differentiation, proliferation and migration processes leading to the development of extra-embryonic membranes, the appearance of a flat embryonic disc and gastrulation. This prolonged preimplantation development is termed conceptus elongation and deficiencies on this process constitute the most frequent cause of reproductive failures in ungulates, including the 4 most relevant mammalian livestock species in Europe. The purpose of this project is to elucidate the factors involved in conceptus elongation by gene editing and in vitro culture approaches. A first objective will be to identify key genes involved in differentiation processes by RNA-seq analysis of different embryo derivatives from bovine conceptuses at different developmental stages. Subsequently, the function of some of the genes identified as well as others known to play a crucial role in mouse development or putatively involved in embryo-maternal interactions will be assessed. For this aim, bovine embryos in which a candidate gene has been ablated (KO) will be generated by CRISPR and transferred to recipient females to assess in vivo the function of such particular gene on conceptus development. A second set of experiments pursue the development of an in vitro system for conceptus elongation that would bypass the requirement for in vivo experiments. For this aim we will perform metabolomics and proteomics analyses of bovine uterine fluid at different stages and will use these data to rationally develop a culture system able to sustain conceptus development. The knowledge generated by this project will serve to develop strategies to enhance farming profitability by reducing embryonic loss and to understand Developmental Biology questions unanswered by the mouse model.

 Deliverables

List of deliverables.
Data Management Plan Open Research Data Pilot 2019-11-25 12:35:12

Take a look to the deliverables list in detail:  detailed list of ELONGAN deliverables.

 Publications

year authors and title journal last update
List of publications.
2018 Ismael Lamas-Toranzo, Eva Pericuesta, Pablo Bermejo-Álvarez
Mitochondrial and metabolic adjustments during the final phase of follicular development prior to IVM of bovine oocytes
published pages: 156-162, ISSN: 0093-691X, DOI: 10.1016/j.theriogenology.2018.07.007
Theriogenology 119 2019-05-22
2018 Ismael Lamas-Toranzo, Priscila Ramos-Ibeas, Eva Pericuesta, Pablo Bermejo-Álvarez
Directions and applications of CRISPR technology in livestock research
published pages: 292-300, ISSN: 1984-3143, DOI: 10.21451/1984-3143-ar2018-0075
Animal Reproduction 15/3 2019-05-22

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The information about "ELONGAN" are provided by the European Opendata Portal: CORDIS opendata.

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