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3DCellPhase- SIGNED

In situ Structural Analysis of Molecular Crowding and Phase Separation

Total Cost €

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EC-Contrib. €

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Partnership

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 3DCellPhase- project word cloud

Explore the words cloud of the 3DCellPhase- project. It provides you a very rough idea of what is the project "3DCellPhase-" about.

eukaryotic    insights    crowded    readout    fibers    brings    windows    enclosed    cells    assemble    intriguingly    assembly    exhibit    volume    combine    eukaryotes    cytoplasmic    rna    sites    living    shows    biology    cryo    cytoplasm    event    vitro    examination    biochemical    fluorescence    stress    beam    excluded    nature    description    ion    variations    question    progress    amorphous    correlative    microscopy    liquids    illusive    local    assemblies    guided    fundamental    elucidate    reconstituting    3d    environment    preparation    context    techniques    dynamically    cellular    prepare    quantitative    liquid    granules    centrosomes    electron    organize    basis    organization    form    bodies    separation    vivo    dynamic    separated    structural    lack    nucleation    molecular    functionally    despite    compartments    tomography    suitable    thinning    driving    gels    principles    site    reactions    membrane    cell    microtubule    direct    triggered    transparent   

Project "3DCellPhase-" data sheet

The following table provides information about the project.

Coordinator
EUROPEAN MOLECULAR BIOLOGY LABORATORY 

Organization address
address: Meyerhofstrasse 1
city: HEIDELBERG
postcode: 69117
website: http://www.embl.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 1˙228˙125 €
 EC max contribution 1˙228˙125 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-STG
 Funding Scheme ERC-STG
 Starting year 2018
 Duration (year-month-day) from 2018-02-01   to  2023-01-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    EUROPEAN MOLECULAR BIOLOGY LABORATORY DE (HEIDELBERG) coordinator 1˙228˙125.00

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 Project objective

This proposal brings together two fields in biology, namely the emerging field of phase-separated assemblies in cell biology and state-of-the-art cellular cryo-electron tomography, to advance our understanding on a fundamental, yet illusive, question: the molecular organization of the cytoplasm.

Eukaryotes organize their biochemical reactions into functionally distinct compartments. Intriguingly, many, if not most, cellular compartments are not membrane enclosed. Rather, they assemble dynamically by phase separation, typically triggered upon a specific event. Despite significant progress on reconstituting such liquid-like assemblies in vitro, we lack information as to whether these compartments in vivo are indeed amorphous liquids, or whether they exhibit structural features such as gels or fibers. My recent work on sample preparation of cells for cryo-electron tomography, including cryo-focused ion beam thinning, guided by 3D correlative fluorescence microscopy, shows that we can now prepare site-specific ‘electron-transparent windows’ in suitable eukaryotic systems, which allow direct examination of structural features of cellular compartments in their cellular context. Here, we will use these techniques to elucidate the structural principles and cytoplasmic environment driving the dynamic assembly of two phase-separated compartments: Stress granules, which are RNA bodies that form rapidly in the cytoplasm upon cellular stress, and centrosomes, which are sites of microtubule nucleation. We will combine these studies with a quantitative description of the crowded nature of cytoplasm and of its local variations, to provide a direct readout of the impact of excluded volume on molecular assembly in living cells. Taken together, these studies will provide fundamental insights into the structural basis by which cells form biochemical compartments.

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The information about "3DCELLPHASE-" are provided by the European Opendata Portal: CORDIS opendata.

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