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3DCellPhase- SIGNED

In situ Structural Analysis of Molecular Crowding and Phase Separation

Total Cost €

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EC-Contrib. €

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Partnership

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 3DCellPhase- project word cloud

Explore the words cloud of the 3DCellPhase- project. It provides you a very rough idea of what is the project "3DCellPhase-" about.

local    tomography    compartments    eukaryotic    cells    fibers    driving    nucleation    suitable    basis    cellular    direct    intriguingly    thinning    correlative    biochemical    shows    cryo    exhibit    ion    brings    environment    enclosed    molecular    readout    insights    rna    separation    microscopy    form    separated    structural    dynamically    despite    progress    context    description    quantitative    assemblies    combine    amorphous    stress    reconstituting    transparent    reactions    elucidate    fundamental    excluded    membrane    assembly    prepare    biology    vitro    organize    electron    cell    variations    gels    centrosomes    examination    illusive    living    volume    liquids    functionally    nature    liquid    granules    guided    windows    eukaryotes    assemble    site    cytoplasm    lack    principles    sites    vivo    fluorescence    microtubule    triggered    question    dynamic    techniques    cytoplasmic    preparation    organization    crowded    event    3d    bodies    beam   

Project "3DCellPhase-" data sheet

The following table provides information about the project.

Coordinator
EUROPEAN MOLECULAR BIOLOGY LABORATORY 

Organization address
address: Meyerhofstrasse 1
city: HEIDELBERG
postcode: 69117
website: http://www.embl.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 1˙228˙125 €
 EC max contribution 1˙228˙125 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-STG
 Funding Scheme ERC-STG
 Starting year 2018
 Duration (year-month-day) from 2018-02-01   to  2023-01-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    EUROPEAN MOLECULAR BIOLOGY LABORATORY DE (HEIDELBERG) coordinator 1˙228˙125.00

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 Project objective

This proposal brings together two fields in biology, namely the emerging field of phase-separated assemblies in cell biology and state-of-the-art cellular cryo-electron tomography, to advance our understanding on a fundamental, yet illusive, question: the molecular organization of the cytoplasm.

Eukaryotes organize their biochemical reactions into functionally distinct compartments. Intriguingly, many, if not most, cellular compartments are not membrane enclosed. Rather, they assemble dynamically by phase separation, typically triggered upon a specific event. Despite significant progress on reconstituting such liquid-like assemblies in vitro, we lack information as to whether these compartments in vivo are indeed amorphous liquids, or whether they exhibit structural features such as gels or fibers. My recent work on sample preparation of cells for cryo-electron tomography, including cryo-focused ion beam thinning, guided by 3D correlative fluorescence microscopy, shows that we can now prepare site-specific ‘electron-transparent windows’ in suitable eukaryotic systems, which allow direct examination of structural features of cellular compartments in their cellular context. Here, we will use these techniques to elucidate the structural principles and cytoplasmic environment driving the dynamic assembly of two phase-separated compartments: Stress granules, which are RNA bodies that form rapidly in the cytoplasm upon cellular stress, and centrosomes, which are sites of microtubule nucleation. We will combine these studies with a quantitative description of the crowded nature of cytoplasm and of its local variations, to provide a direct readout of the impact of excluded volume on molecular assembly in living cells. Taken together, these studies will provide fundamental insights into the structural basis by which cells form biochemical compartments.

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The information about "3DCELLPHASE-" are provided by the European Opendata Portal: CORDIS opendata.

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