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3DCellPhase- SIGNED

In situ Structural Analysis of Molecular Crowding and Phase Separation

Total Cost €

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EC-Contrib. €

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Partnership

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 3DCellPhase- project word cloud

Explore the words cloud of the 3DCellPhase- project. It provides you a very rough idea of what is the project "3DCellPhase-" about.

molecular    separation    correlative    functionally    crowded    excluded    insights    structural    stress    vitro    illusive    site    organize    membrane    volume    dynamic    reactions    exhibit    guided    lack    cytoplasm    form    reconstituting    ion    fibers    thinning    direct    eukaryotic    transparent    fluorescence    organization    separated    microscopy    shows    examination    fundamental    enclosed    gels    sites    tomography    assembly    suitable    centrosomes    nature    intriguingly    progress    combine    techniques    granules    beam    cryo    preparation    quantitative    basis    environment    vivo    cytoplasmic    dynamically    despite    bodies    compartments    liquids    driving    assemblies    eukaryotes    variations    description    context    3d    triggered    biology    question    cellular    brings    rna    event    electron    assemble    cells    windows    local    living    nucleation    liquid    biochemical    principles    elucidate    microtubule    amorphous    prepare    readout    cell   

Project "3DCellPhase-" data sheet

The following table provides information about the project.

Coordinator
EUROPEAN MOLECULAR BIOLOGY LABORATORY 

Organization address
address: Meyerhofstrasse 1
city: HEIDELBERG
postcode: 69117
website: http://www.embl.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 1˙228˙125 €
 EC max contribution 1˙228˙125 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-STG
 Funding Scheme ERC-STG
 Starting year 2018
 Duration (year-month-day) from 2018-02-01   to  2023-01-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    EUROPEAN MOLECULAR BIOLOGY LABORATORY DE (HEIDELBERG) coordinator 1˙228˙125.00

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 Project objective

This proposal brings together two fields in biology, namely the emerging field of phase-separated assemblies in cell biology and state-of-the-art cellular cryo-electron tomography, to advance our understanding on a fundamental, yet illusive, question: the molecular organization of the cytoplasm.

Eukaryotes organize their biochemical reactions into functionally distinct compartments. Intriguingly, many, if not most, cellular compartments are not membrane enclosed. Rather, they assemble dynamically by phase separation, typically triggered upon a specific event. Despite significant progress on reconstituting such liquid-like assemblies in vitro, we lack information as to whether these compartments in vivo are indeed amorphous liquids, or whether they exhibit structural features such as gels or fibers. My recent work on sample preparation of cells for cryo-electron tomography, including cryo-focused ion beam thinning, guided by 3D correlative fluorescence microscopy, shows that we can now prepare site-specific ‘electron-transparent windows’ in suitable eukaryotic systems, which allow direct examination of structural features of cellular compartments in their cellular context. Here, we will use these techniques to elucidate the structural principles and cytoplasmic environment driving the dynamic assembly of two phase-separated compartments: Stress granules, which are RNA bodies that form rapidly in the cytoplasm upon cellular stress, and centrosomes, which are sites of microtubule nucleation. We will combine these studies with a quantitative description of the crowded nature of cytoplasm and of its local variations, to provide a direct readout of the impact of excluded volume on molecular assembly in living cells. Taken together, these studies will provide fundamental insights into the structural basis by which cells form biochemical compartments.

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The information about "3DCELLPHASE-" are provided by the European Opendata Portal: CORDIS opendata.

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