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3DCellPhase- SIGNED

In situ Structural Analysis of Molecular Crowding and Phase Separation

Total Cost €

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EC-Contrib. €

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Partnership

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 3DCellPhase- project word cloud

Explore the words cloud of the 3DCellPhase- project. It provides you a very rough idea of what is the project "3DCellPhase-" about.

combine    cytoplasm    assemblies    liquid    cell    dynamic    insights    exhibit    assembly    basis    rna    membrane    nature    progress    molecular    reconstituting    cells    separated    tomography    elucidate    readout    techniques    functionally    preparation    liquids    lack    dynamically    biology    description    local    direct    separation    eukaryotic    fibers    triggered    beam    centrosomes    crowded    volume    cellular    compartments    vivo    suitable    variations    vitro    amorphous    eukaryotes    illusive    site    gels    ion    thinning    driving    transparent    intriguingly    despite    organize    excluded    cytoplasmic    granules    nucleation    stress    living    microtubule    3d    prepare    sites    event    enclosed    fluorescence    brings    assemble    question    biochemical    examination    cryo    electron    quantitative    reactions    structural    microscopy    form    correlative    bodies    organization    environment    fundamental    shows    principles    context    windows    guided   

Project "3DCellPhase-" data sheet

The following table provides information about the project.

Coordinator
EUROPEAN MOLECULAR BIOLOGY LABORATORY 

Organization address
address: Meyerhofstrasse 1
city: HEIDELBERG
postcode: 69117
website: http://www.embl.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 1˙228˙125 €
 EC max contribution 1˙228˙125 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-STG
 Funding Scheme ERC-STG
 Starting year 2018
 Duration (year-month-day) from 2018-02-01   to  2023-01-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    EUROPEAN MOLECULAR BIOLOGY LABORATORY DE (HEIDELBERG) coordinator 1˙228˙125.00

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 Project objective

This proposal brings together two fields in biology, namely the emerging field of phase-separated assemblies in cell biology and state-of-the-art cellular cryo-electron tomography, to advance our understanding on a fundamental, yet illusive, question: the molecular organization of the cytoplasm.

Eukaryotes organize their biochemical reactions into functionally distinct compartments. Intriguingly, many, if not most, cellular compartments are not membrane enclosed. Rather, they assemble dynamically by phase separation, typically triggered upon a specific event. Despite significant progress on reconstituting such liquid-like assemblies in vitro, we lack information as to whether these compartments in vivo are indeed amorphous liquids, or whether they exhibit structural features such as gels or fibers. My recent work on sample preparation of cells for cryo-electron tomography, including cryo-focused ion beam thinning, guided by 3D correlative fluorescence microscopy, shows that we can now prepare site-specific ‘electron-transparent windows’ in suitable eukaryotic systems, which allow direct examination of structural features of cellular compartments in their cellular context. Here, we will use these techniques to elucidate the structural principles and cytoplasmic environment driving the dynamic assembly of two phase-separated compartments: Stress granules, which are RNA bodies that form rapidly in the cytoplasm upon cellular stress, and centrosomes, which are sites of microtubule nucleation. We will combine these studies with a quantitative description of the crowded nature of cytoplasm and of its local variations, to provide a direct readout of the impact of excluded volume on molecular assembly in living cells. Taken together, these studies will provide fundamental insights into the structural basis by which cells form biochemical compartments.

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The information about "3DCELLPHASE-" are provided by the European Opendata Portal: CORDIS opendata.

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