SPICE SIGNED
Spectroscopy in cells with tailored in-vivo labelling strategies and multiply addressable nano-structural probes
Total Cost €
0EC-Contrib. €
0Partnership
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Project "SPICE" data sheet
The following table provides information about the project.
| Coordinator |
UNIVERSITAT KONSTANZ
Organization address contact info |
| Coordinator Country | Germany [DE] |
| Total cost | 1˙988˙697 € |
| EC max contribution | 1˙988˙697 € (100%) |
| Programme |
1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC)) |
| Code Call | ERC-2017-COG |
| Funding Scheme | ERC-COG |
| Starting year | 2018 |
| Duration (year-month-day) | from 2018-06-01 to 2023-05-31 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | UNIVERSITAT KONSTANZ | DE (KONSTANZ) | coordinator | 1˙988˙697.00 |
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Project objective
This project proposes to combine complementary spectroscopic approaches and push the domain of molecular spectroscopy for structural biology and biophysics into the living cell.
Non-invasive molecular spectroscopy to probe protein dynamics and structures at the molecular level is essential for unravelling one of the most challenges in Chemical Biology - the complex physical mechanism of life. Of particular interest are electron paramagnetic resonance (EPR) distance measurements in the nanometer range, especially, when they will be combined with complementary spectroscopic techniques. By now, different spectroscopic approaches require specific labelling strategies and probes. In this project, we will develop multiply-addressable nano-structural probes that are in parallel suitable for various spectroscopic techniques, e.g. magnetic resonance spectroscopy (NMR and EPR), vibrational spectroscopy, and optical (including single molecule) spectroscopy and microscopy. This enables obtaining information on all relevant length and time scales for the very same sample. Labelling will be performed directly in the native intracellular environment, where the proteins are folded, modified, transferred and degraded. Corresponding spectroscopic experiments can be combined or re-arranged for novel experiments even in cellula.
Current characterization methods for structure and dynamics of proteins are generally applied in vitro. In the cell, posttranslational modifications, crowding effects, organelle specific localization, or non-specific or specific interactions with cellular components significantly affect structure and conformational equilibria of proteins. We will demonstrate that novel in-cell spectroscopy approaches developed within the SPICE-project using multiply-addressable nano-structural probes and in vivo labelling strategies will open up possibilities to explore larger and more complex biological structures at the molecular level in cellula, which has hitherto been
Publications
| year | authors and title | journal | last update |
|---|---|---|---|
| 2019 |
Mykhailo Azarkh, Anna Bieber, Mian Qi, Jörg W. A. Fischer, Maxim Yulikov, Adelheid Godt, Malte Drescher Gd(III)–Gd(III) Relaxation-Induced Dipolar Modulation Enhancement for In-Cell Electron Paramagnetic Resonance Distance Determination published pages: 1477-1481, ISSN: 1948-7185, DOI: 10.1021/acs.jpclett.9b00340 |
The Journal of Physical Chemistry Letters 10/7 | 2019-08-29 |
| 2019 |
Pia Widder, Frederic Berner, Daniel Summerer, Malte Drescher Double Nitroxide Labeling by Copper-Catalyzed Azide–Alkyne Cycloadditions with Noncanonical Amino Acids for Electron Paramagnetic Resonance Spectroscopy published pages: 839-844, ISSN: 1554-8929, DOI: 10.1021/acschembio.8b01111 |
ACS Chemical Biology 14/5 | 2019-08-29 |
| 2018 |
Anna Bieber, Dennis Bücker, Malte Drescher Light-induced dipolar spectroscopy – A quantitative comparison between LiDEER and LaserIMD published pages: 29-35, ISSN: 1090-7807, DOI: 10.1016/j.jmr.2018.08.006 |
Journal of Magnetic Resonance 296 | 2019-08-29 |
| 2019 |
Anandi Kugele, Bjarne Silkenath, Jakob Langer, Valentin Wittmann, Malte Drescher Protein Spin Labeling with a Photocaged Nitroxide Using Diels–Alder Chemistry published pages: , ISSN: 1439-4227, DOI: 10.1002/cbic.201900318 |
ChemBioChem | 2019-08-29 |
| 2019 |
Anandi Kugele, Theresa Sophie Braun, Pia Widder, Lara Williams, Moritz Johannes Schmidt, Daniel Summerer, Malte Drescher Site-directed spin labelling of proteins by Suzuki–Miyaura coupling via a genetically encoded aryliodide amino acid published pages: 1923-1926, ISSN: 1359-7345, DOI: 10.1039/c8cc09325c |
Chemical Communications 55/13 | 2019-08-29 |
| 2019 |
Theresa Braun, Malte Drescher, Daniel Summerer Expanding the Genetic Code for Site-Directed Spin-Labeling published pages: 373, ISSN: 1422-0067, DOI: 10.3390/ijms20020373 |
International Journal of Molecular Sciences 20/2 | 2019-08-29 |
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