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SPICE SIGNED

Spectroscopy in cells with tailored in-vivo labelling strategies and multiply addressable nano-structural probes

Total Cost €

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EC-Contrib. €

0

Partnership

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 SPICE project word cloud

Explore the words cloud of the SPICE project. It provides you a very rough idea of what is the project "SPICE" about.

posttranslational    mechanism    suitable    multiply    length    cell    epr    combined    folded    complementary    protein    crowding    optical    vitro    re    nano    corresponding    probes    scales    spectroscopy    life    invasive    chemical    significantly    molecule    been    performed    electron    biology    dynamics    molecular    conformational    microscopy    nmr    structures    combine    equilibria    domain    structure    proposes    modified    unravelling    larger    explore    nanometer    intracellular    parallel    hitherto    components    specific    time    environment    characterization    vivo    native    biophysics    single    cellula    generally    living    magnetic    localization    transferred    structural    resonance    obtaining    physical    probe    biological    vibrational    paramagnetic    distance    degraded    addressable    spectroscopic    interactions    techniques    proteins    cellular    strategies    spice    modifications    experiments    arranged    possibilities    organelle    labelling    push   

Project "SPICE" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITAT KONSTANZ 

Organization address
address: UNIVERSITATSSTRASSE 10
city: KONSTANZ
postcode: 78464
website: www.uni-konstanz.de/

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 1˙988˙697 €
 EC max contribution 1˙988˙697 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-COG
 Funding Scheme ERC-COG
 Starting year 2018
 Duration (year-month-day) from 2018-06-01   to  2023-05-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITAT KONSTANZ DE (KONSTANZ) coordinator 1˙988˙697.00

Map

 Project objective

This project proposes to combine complementary spectroscopic approaches and push the domain of molecular spectroscopy for structural biology and biophysics into the living cell.

Non-invasive molecular spectroscopy to probe protein dynamics and structures at the molecular level is essential for unravelling one of the most challenges in Chemical Biology - the complex physical mechanism of life. Of particular interest are electron paramagnetic resonance (EPR) distance measurements in the nanometer range, especially, when they will be combined with complementary spectroscopic techniques. By now, different spectroscopic approaches require specific labelling strategies and probes. In this project, we will develop multiply-addressable nano-structural probes that are in parallel suitable for various spectroscopic techniques, e.g. magnetic resonance spectroscopy (NMR and EPR), vibrational spectroscopy, and optical (including single molecule) spectroscopy and microscopy. This enables obtaining information on all relevant length and time scales for the very same sample. Labelling will be performed directly in the native intracellular environment, where the proteins are folded, modified, transferred and degraded. Corresponding spectroscopic experiments can be combined or re-arranged for novel experiments even in cellula.

Current characterization methods for structure and dynamics of proteins are generally applied in vitro. In the cell, posttranslational modifications, crowding effects, organelle specific localization, or non-specific or specific interactions with cellular components significantly affect structure and conformational equilibria of proteins. We will demonstrate that novel in-cell spectroscopy approaches developed within the SPICE-project using multiply-addressable nano-structural probes and in vivo labelling strategies will open up possibilities to explore larger and more complex biological structures at the molecular level in cellula, which has hitherto been

 Publications

year authors and title journal last update
List of publications.
2019 Mykhailo Azarkh, Anna Bieber, Mian Qi, Jörg W. A. Fischer, Maxim Yulikov, Adelheid Godt, Malte Drescher
Gd(III)–Gd(III) Relaxation-Induced Dipolar Modulation Enhancement for In-Cell Electron Paramagnetic Resonance Distance Determination
published pages: 1477-1481, ISSN: 1948-7185, DOI: 10.1021/acs.jpclett.9b00340
The Journal of Physical Chemistry Letters 10/7 2019-08-29
2019 Pia Widder, Frederic Berner, Daniel Summerer, Malte Drescher
Double Nitroxide Labeling by Copper-Catalyzed Azide–Alkyne Cycloadditions with Noncanonical Amino Acids for Electron Paramagnetic Resonance Spectroscopy
published pages: 839-844, ISSN: 1554-8929, DOI: 10.1021/acschembio.8b01111
ACS Chemical Biology 14/5 2019-08-29
2018 Anna Bieber, Dennis Bücker, Malte Drescher
Light-induced dipolar spectroscopy – A quantitative comparison between LiDEER and LaserIMD
published pages: 29-35, ISSN: 1090-7807, DOI: 10.1016/j.jmr.2018.08.006
Journal of Magnetic Resonance 296 2019-08-29
2019 Anandi Kugele, Bjarne Silkenath, Jakob Langer, Valentin Wittmann, Malte Drescher
Protein Spin Labeling with a Photocaged Nitroxide Using Diels–Alder Chemistry
published pages: , ISSN: 1439-4227, DOI: 10.1002/cbic.201900318
ChemBioChem 2019-08-29
2019 Anandi Kugele, Theresa Sophie Braun, Pia Widder, Lara Williams, Moritz Johannes Schmidt, Daniel Summerer, Malte Drescher
Site-directed spin labelling of proteins by Suzuki–Miyaura coupling via a genetically encoded aryliodide amino acid
published pages: 1923-1926, ISSN: 1359-7345, DOI: 10.1039/c8cc09325c
Chemical Communications 55/13 2019-08-29
2019 Theresa Braun, Malte Drescher, Daniel Summerer
Expanding the Genetic Code for Site-Directed Spin-Labeling
published pages: 373, ISSN: 1422-0067, DOI: 10.3390/ijms20020373
International Journal of Molecular Sciences 20/2 2019-08-29

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