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Spectroscopy in cells with tailored in-vivo labelling strategies and multiply addressable nano-structural probes

Total Cost €


EC-Contrib. €






 SPICE project word cloud

Explore the words cloud of the SPICE project. It provides you a very rough idea of what is the project "SPICE" about.

proteins    dynamics    unravelling    arranged    cellular    suitable    techniques    magnetic    experiments    probes    invasive    epr    conformational    localization    chemical    resonance    spectroscopic    proposes    modified    obtaining    molecule    domain    degraded    living    native    biology    electron    physical    cellula    mechanism    multiply    been    paramagnetic    vibrational    nano    protein    combine    interactions    specific    probe    performed    structural    combined    intracellular    biological    nanometer    components    complementary    optical    equilibria    folded    push    parallel    characterization    explore    possibilities    time    transferred    length    single    life    corresponding    addressable    organelle    larger    distance    hitherto    environment    nmr    spice    scales    significantly    vitro    cell    structures    re    strategies    generally    modifications    crowding    microscopy    labelling    biophysics    spectroscopy    molecular    structure    posttranslational    vivo   

Project "SPICE" data sheet

The following table provides information about the project.


Organization address
postcode: 78464

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 1˙988˙697 €
 EC max contribution 1˙988˙697 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-COG
 Funding Scheme ERC-COG
 Starting year 2018
 Duration (year-month-day) from 2018-06-01   to  2023-05-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITAT KONSTANZ DE (KONSTANZ) coordinator 1˙988˙697.00


 Project objective

This project proposes to combine complementary spectroscopic approaches and push the domain of molecular spectroscopy for structural biology and biophysics into the living cell.

Non-invasive molecular spectroscopy to probe protein dynamics and structures at the molecular level is essential for unravelling one of the most challenges in Chemical Biology - the complex physical mechanism of life. Of particular interest are electron paramagnetic resonance (EPR) distance measurements in the nanometer range, especially, when they will be combined with complementary spectroscopic techniques. By now, different spectroscopic approaches require specific labelling strategies and probes. In this project, we will develop multiply-addressable nano-structural probes that are in parallel suitable for various spectroscopic techniques, e.g. magnetic resonance spectroscopy (NMR and EPR), vibrational spectroscopy, and optical (including single molecule) spectroscopy and microscopy. This enables obtaining information on all relevant length and time scales for the very same sample. Labelling will be performed directly in the native intracellular environment, where the proteins are folded, modified, transferred and degraded. Corresponding spectroscopic experiments can be combined or re-arranged for novel experiments even in cellula.

Current characterization methods for structure and dynamics of proteins are generally applied in vitro. In the cell, posttranslational modifications, crowding effects, organelle specific localization, or non-specific or specific interactions with cellular components significantly affect structure and conformational equilibria of proteins. We will demonstrate that novel in-cell spectroscopy approaches developed within the SPICE-project using multiply-addressable nano-structural probes and in vivo labelling strategies will open up possibilities to explore larger and more complex biological structures at the molecular level in cellula, which has hitherto been


year authors and title journal last update
List of publications.
2019 Mykhailo Azarkh, Anna Bieber, Mian Qi, Jörg W. A. Fischer, Maxim Yulikov, Adelheid Godt, Malte Drescher
Gd(III)–Gd(III) Relaxation-Induced Dipolar Modulation Enhancement for In-Cell Electron Paramagnetic Resonance Distance Determination
published pages: 1477-1481, ISSN: 1948-7185, DOI: 10.1021/acs.jpclett.9b00340
The Journal of Physical Chemistry Letters 10/7 2019-08-29
2019 Pia Widder, Frederic Berner, Daniel Summerer, Malte Drescher
Double Nitroxide Labeling by Copper-Catalyzed Azide–Alkyne Cycloadditions with Noncanonical Amino Acids for Electron Paramagnetic Resonance Spectroscopy
published pages: 839-844, ISSN: 1554-8929, DOI: 10.1021/acschembio.8b01111
ACS Chemical Biology 14/5 2019-08-29
2018 Anna Bieber, Dennis Bücker, Malte Drescher
Light-induced dipolar spectroscopy – A quantitative comparison between LiDEER and LaserIMD
published pages: 29-35, ISSN: 1090-7807, DOI: 10.1016/j.jmr.2018.08.006
Journal of Magnetic Resonance 296 2019-08-29
2019 Anandi Kugele, Bjarne Silkenath, Jakob Langer, Valentin Wittmann, Malte Drescher
Protein Spin Labeling with a Photocaged Nitroxide Using Diels–Alder Chemistry
published pages: , ISSN: 1439-4227, DOI: 10.1002/cbic.201900318
ChemBioChem 2019-08-29
2019 Anandi Kugele, Theresa Sophie Braun, Pia Widder, Lara Williams, Moritz Johannes Schmidt, Daniel Summerer, Malte Drescher
Site-directed spin labelling of proteins by Suzuki–Miyaura coupling via a genetically encoded aryliodide amino acid
published pages: 1923-1926, ISSN: 1359-7345, DOI: 10.1039/c8cc09325c
Chemical Communications 55/13 2019-08-29
2019 Theresa Braun, Malte Drescher, Daniel Summerer
Expanding the Genetic Code for Site-Directed Spin-Labeling
published pages: 373, ISSN: 1422-0067, DOI: 10.3390/ijms20020373
International Journal of Molecular Sciences 20/2 2019-08-29

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