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PotatoMASH SIGNED

Potato Multi-Allele Scanning Haplotags – a low cost, genome-scanning marker system for use in potato breeding

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 PotatoMASH project word cloud

Explore the words cloud of the PotatoMASH project. It provides you a very rough idea of what is the project "PotatoMASH" about.

generation    unlike    reads    utilised    sequencing    1000    genotyping    genome    faster    single    fry    date    placed    profile    platform    commercial    rate    clusters    assayed    yield    variants    deploying    simultaneous    combining    paired    loci    breeder    bi    utilisation    conjunction    colour    restrict    thousands    burden    fellowship    spacing    gs    run    portion    genetic    sustainability    efficient    linked    scanning    employ    tetraploid    strategies    disequilibrium    varieties    samples    gain    pcr    distances    plant    economic    genomic    discriminate    patterns    assisted    gt    barcoding    haplotags    subsequently    multiplex    skills    uses    euchromatic    potato    snps    ngs    aggregate    1mb    training    breeding    traits    populations    linkage    mas    markers    approximately    tended    prediction    adoption    researcher    seq    postdoctoral    covered    combined    combinatorial    equip    400    marker    allelic    equations    plants    annually    multiple    young    action    multiplexing    massively    combine   

Project "PotatoMASH" data sheet

The following table provides information about the project.

Coordinator
TEAGASC - AGRICULTURE AND FOOD DEVELOPMENT AUTHORITY 

Organization address
address: Oak Park
city: Carlow
postcode: R93
website: n.a.

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Ireland [IE]
 Total cost 175˙866 €
 EC max contribution 175˙866 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2017
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-09-24   to  2020-09-23

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    TEAGASC - AGRICULTURE AND FOOD DEVELOPMENT AUTHORITY IE (Carlow) coordinator 175˙866.00

Map

 Project objective

'Potato breeding is a 10-year process that involves combining over 40 characteristics to produce varieties that have improved sustainability, utilisation and consumer characteristics. Genomic and marker assisted selection (GS and MAS) can make breeding faster/more efficient, increasing the rate of genetic gain and the ability to combine multiple traits. Strategies for GS to date have tended to employ many thousands of markers; however, the economic burden of deploying such approaches on thousands of plants annually in a breeding programme may restrict the adoption of GS. The objective of this action is to develop a novel, low cost, genome-scanning marker platform for use in simultaneous GS and MAS for multiple traits in potato breeding, by combining existing knowledge on the patterns of linkage disequilibrium in potato with recent advances in genotyping-by-sequencing approaches based on massively multiplex PCR. The marker system will target clusters of SNPs whose aggregate profile over distances covered by paired-end next generation sequencing (NGS) reads will allow them to be used as haplotags. Unlike bi-allelic SNPs, haplotags can discriminate multiple allelic variants in tetraploid potato. Approximately 400 loci at placed at 1Mb spacing throughout the euchromatic portion of the genome, and a further 100 SNPs linked to specific traits, will be targeted and assayed using an approach called GT-Seq, which uses combinatorial barcoding to allow multiplexing of thousands of samples in a single NGS run. The sequencing platform will be utilised in conjunction with 2 training populations to develop genomic prediction equations for yield and fry colour of potato. Subsequently, it will be deployed for combined GS and MAS on 1000 plants from the second field generation of a commercial potato breeding programme. The research and training activities in the fellowship proposal will equip a young postdoctoral researcher with the skills of a 'next generation plant breeder'.

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The information about "POTATOMASH" are provided by the European Opendata Portal: CORDIS opendata.

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