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PotatoMASH SIGNED

Potato Multi-Allele Scanning Haplotags – a low cost, genome-scanning marker system for use in potato breeding

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 PotatoMASH project word cloud

Explore the words cloud of the PotatoMASH project. It provides you a very rough idea of what is the project "PotatoMASH" about.

skills    plants    tended    seq    reads    fellowship    date    spacing    assisted    single    400    annually    gt    paired    fry    genetic    run    portion    marker    distances    plant    generation    linked    equations    strategies    unlike    genomic    yield    placed    training    snps    potato    aggregate    multiplex    subsequently    haplotags    bi    deploying    barcoding    populations    adoption    gain    action    covered    scanning    commercial    utilisation    prediction    rate    disequilibrium    breeding    sequencing    loci    thousands    utilised    multiplexing    patterns    researcher    employ    1000    assayed    restrict    multiple    equip    varieties    mas    postdoctoral    uses    gs    1mb    markers    traits    young    samples    variants    faster    combined    profile    platform    massively    combine    combinatorial    efficient    conjunction    economic    colour    burden    euchromatic    breeder    ngs    genome    approximately    tetraploid    discriminate    pcr    clusters    combining    linkage    simultaneous    sustainability    genotyping    allelic   

Project "PotatoMASH" data sheet

The following table provides information about the project.

Coordinator
TEAGASC - AGRICULTURE AND FOOD DEVELOPMENT AUTHORITY 

Organization address
address: Oak Park
city: Carlow
postcode: R93
website: n.a.

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Ireland [IE]
 Total cost 175˙866 €
 EC max contribution 175˙866 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2017
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-09-24   to  2020-09-23

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    TEAGASC - AGRICULTURE AND FOOD DEVELOPMENT AUTHORITY IE (Carlow) coordinator 175˙866.00

Map

 Project objective

'Potato breeding is a 10-year process that involves combining over 40 characteristics to produce varieties that have improved sustainability, utilisation and consumer characteristics. Genomic and marker assisted selection (GS and MAS) can make breeding faster/more efficient, increasing the rate of genetic gain and the ability to combine multiple traits. Strategies for GS to date have tended to employ many thousands of markers; however, the economic burden of deploying such approaches on thousands of plants annually in a breeding programme may restrict the adoption of GS. The objective of this action is to develop a novel, low cost, genome-scanning marker platform for use in simultaneous GS and MAS for multiple traits in potato breeding, by combining existing knowledge on the patterns of linkage disequilibrium in potato with recent advances in genotyping-by-sequencing approaches based on massively multiplex PCR. The marker system will target clusters of SNPs whose aggregate profile over distances covered by paired-end next generation sequencing (NGS) reads will allow them to be used as haplotags. Unlike bi-allelic SNPs, haplotags can discriminate multiple allelic variants in tetraploid potato. Approximately 400 loci at placed at 1Mb spacing throughout the euchromatic portion of the genome, and a further 100 SNPs linked to specific traits, will be targeted and assayed using an approach called GT-Seq, which uses combinatorial barcoding to allow multiplexing of thousands of samples in a single NGS run. The sequencing platform will be utilised in conjunction with 2 training populations to develop genomic prediction equations for yield and fry colour of potato. Subsequently, it will be deployed for combined GS and MAS on 1000 plants from the second field generation of a commercial potato breeding programme. The research and training activities in the fellowship proposal will equip a young postdoctoral researcher with the skills of a 'next generation plant breeder'.

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The information about "POTATOMASH" are provided by the European Opendata Portal: CORDIS opendata.

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