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INTERSPREC SIGNED

Investigation of interspecific pollen tube reception

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 INTERSPREC project word cloud

Explore the words cloud of the INTERSPREC project. It provides you a very rough idea of what is the project "INTERSPREC" about.

player    cis    preliminary    arabidopsis    interactions    attest    screening    mechanisms    interaction    regulatory    output    pts    codes    causing    organisms    distinction    recognize    plants    fungi    female    perfectly    glycan    manipulate    host    at5g62150    differences    reproduction    dissect    gene    pointing    symbiosis    binding    acid    recognition    pave    peptide    reception    identification    animals    lysm    barriers    chip    fundamental    accessions    sperm    pt    plant    responsible    certain    cell    acting    shed    release    regulators    manipulating    gametophyte    contrast    lyrata    powerful    function    profile    thaliana    pollen    containing    differ    mechanism    biological    protein    instrument    play    y1h    almost    amino    expression    first    communication    male    light    found    correlate    crosses    sequence    trans    immunity    lab    species    symbiotic    ways    patterns    reverse    tube    domain    regulation    interspecific    fertilization    dna    bacteria    breeding    glycosylation   

Project "INTERSPREC" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITAT ZURICH 

Organization address
address: RAMISTRASSE 71
city: ZURICH
postcode: 8006
website: n.a.

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Total cost 175˙419 €
 EC max contribution 175˙419 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2017
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-07-01   to  2020-06-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITAT ZURICH CH (ZURICH) coordinator 175˙419.00

Map

 Project objective

Cell-cell interaction mechanisms play a role in many fundamental biological processes from symbiotic interactions of fungi and bacteria with their host plants to fertilization both in animals and plants. In plant reproduction, pollen tube (PT) reception is a cell-cell communication process allowing the recognition of the male gametophyte (the PT) by the female gametophyte necessary for fertilization. To investigate this process, the host lab used interspecific crosses between certain Arabidopsis thaliana accessions and A.lyrata pollen, where most PTs are not recognized, causing a failure in sperm release and thus fertilization. In contrast, several other A.thaliana accessions almost perfectly recognize interspecific PTs. Preliminary results identified the gene At5g62150 as a key player for species-specific PT recognition. At5g62150 codes for a LysM-domain containing glycan-binding peptide, pointing at glycosylation patterns for the distinction of intra- and interspecific PTs, a mechanism already known to be important in symbiosis and immunity. It was found that differences in the regulation of At5g62150 in distinct species is responsible for recognition, as the amino acid sequence does not differ between A. thaliana other accessions. With this proposal, we will first attest and manipulate the expression profile of At5g62150 in different accessions to correlate its regulation with its function. Then, we propose to dissect the regulation of At5g62150 in different ways: identification and study of the essential cis-regulatory element for regulation and Y1H screening and Reverse ChIP to find trans-acting regulators. The output will not only pave the way for the understanding and manipulating crosses barriers in plants, which is highly relevant for plant breeding, and shed light on cell-cell communication processes in general, but also will establish a novel, powerful instrument (Reverse ChIP) to investigate DNA-protein interaction in complex organisms.

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The information about "INTERSPREC" are provided by the European Opendata Portal: CORDIS opendata.

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