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INTERSPREC SIGNED

Investigation of interspecific pollen tube reception

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 INTERSPREC project word cloud

Explore the words cloud of the INTERSPREC project. It provides you a very rough idea of what is the project "INTERSPREC" about.

mechanism    play    dna    instrument    perfectly    sequence    crosses    reception    gene    at5g62150    reverse    lysm    release    mechanisms    binding    distinction    shed    pave    manipulate    cis    differences    cell    causing    dissect    gametophyte    sperm    barriers    biological    reproduction    contrast    species    light    lab    interactions    animals    codes    regulation    function    powerful    protein    recognition    fundamental    domain    communication    pointing    pollen    containing    acting    fungi    immunity    regulators    interspecific    arabidopsis    profile    male    symbiosis    accessions    first    regulatory    bacteria    plant    glycosylation    certain    found    patterns    plants    attest    responsible    expression    differ    recognize    interaction    ways    acid    tube    screening    y1h    correlate    almost    glycan    organisms    lyrata    trans    chip    preliminary    output    fertilization    pt    amino    peptide    identification    manipulating    symbiotic    host    breeding    female    pts    thaliana    player   

Project "INTERSPREC" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITAT ZURICH 

Organization address
address: RAMISTRASSE 71
city: ZURICH
postcode: 8006
website: n.a.

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Total cost 175˙419 €
 EC max contribution 175˙419 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2017
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-07-01   to  2020-06-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITAT ZURICH CH (ZURICH) coordinator 175˙419.00

Map

 Project objective

Cell-cell interaction mechanisms play a role in many fundamental biological processes from symbiotic interactions of fungi and bacteria with their host plants to fertilization both in animals and plants. In plant reproduction, pollen tube (PT) reception is a cell-cell communication process allowing the recognition of the male gametophyte (the PT) by the female gametophyte necessary for fertilization. To investigate this process, the host lab used interspecific crosses between certain Arabidopsis thaliana accessions and A.lyrata pollen, where most PTs are not recognized, causing a failure in sperm release and thus fertilization. In contrast, several other A.thaliana accessions almost perfectly recognize interspecific PTs. Preliminary results identified the gene At5g62150 as a key player for species-specific PT recognition. At5g62150 codes for a LysM-domain containing glycan-binding peptide, pointing at glycosylation patterns for the distinction of intra- and interspecific PTs, a mechanism already known to be important in symbiosis and immunity. It was found that differences in the regulation of At5g62150 in distinct species is responsible for recognition, as the amino acid sequence does not differ between A. thaliana other accessions. With this proposal, we will first attest and manipulate the expression profile of At5g62150 in different accessions to correlate its regulation with its function. Then, we propose to dissect the regulation of At5g62150 in different ways: identification and study of the essential cis-regulatory element for regulation and Y1H screening and Reverse ChIP to find trans-acting regulators. The output will not only pave the way for the understanding and manipulating crosses barriers in plants, which is highly relevant for plant breeding, and shed light on cell-cell communication processes in general, but also will establish a novel, powerful instrument (Reverse ChIP) to investigate DNA-protein interaction in complex organisms.

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The information about "INTERSPREC" are provided by the European Opendata Portal: CORDIS opendata.

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lastchecktime (2020-11-26 16:19:46) correctly updated