Opendata, web and dolomites


Investigation of interspecific pollen tube reception

Total Cost €


EC-Contrib. €






 INTERSPREC project word cloud

Explore the words cloud of the INTERSPREC project. It provides you a very rough idea of what is the project "INTERSPREC" about.

play    fungi    patterns    host    at5g62150    reproduction    differ    causing    cis    pt    responsible    acid    accessions    symbiotic    screening    correlate    animals    found    thaliana    amino    sperm    bacteria    male    regulators    immunity    biological    trans    binding    certain    differences    profile    interactions    plants    pointing    lab    manipulating    communication    function    glycosylation    mechanism    regulatory    plant    recognition    organisms    release    acting    fertilization    containing    gametophyte    powerful    preliminary    expression    shed    sequence    distinction    domain    dna    barriers    identification    glycan    perfectly    contrast    reception    symbiosis    instrument    player    pave    regulation    tube    arabidopsis    female    recognize    breeding    mechanisms    crosses    peptide    reverse    y1h    first    gene    codes    chip    pollen    manipulate    pts    species    cell    output    lyrata    lysm    protein    almost    light    interspecific    fundamental    attest    ways    interaction    dissect   

Project "INTERSPREC" data sheet

The following table provides information about the project.


Organization address
address: RAMISTRASSE 71
city: ZURICH
postcode: 8006
website: n.a.

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Total cost 175˙419 €
 EC max contribution 175˙419 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2017
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-07-01   to  2020-06-30


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITAT ZURICH CH (ZURICH) coordinator 175˙419.00


 Project objective

Cell-cell interaction mechanisms play a role in many fundamental biological processes from symbiotic interactions of fungi and bacteria with their host plants to fertilization both in animals and plants. In plant reproduction, pollen tube (PT) reception is a cell-cell communication process allowing the recognition of the male gametophyte (the PT) by the female gametophyte necessary for fertilization. To investigate this process, the host lab used interspecific crosses between certain Arabidopsis thaliana accessions and A.lyrata pollen, where most PTs are not recognized, causing a failure in sperm release and thus fertilization. In contrast, several other A.thaliana accessions almost perfectly recognize interspecific PTs. Preliminary results identified the gene At5g62150 as a key player for species-specific PT recognition. At5g62150 codes for a LysM-domain containing glycan-binding peptide, pointing at glycosylation patterns for the distinction of intra- and interspecific PTs, a mechanism already known to be important in symbiosis and immunity. It was found that differences in the regulation of At5g62150 in distinct species is responsible for recognition, as the amino acid sequence does not differ between A. thaliana other accessions. With this proposal, we will first attest and manipulate the expression profile of At5g62150 in different accessions to correlate its regulation with its function. Then, we propose to dissect the regulation of At5g62150 in different ways: identification and study of the essential cis-regulatory element for regulation and Y1H screening and Reverse ChIP to find trans-acting regulators. The output will not only pave the way for the understanding and manipulating crosses barriers in plants, which is highly relevant for plant breeding, and shed light on cell-cell communication processes in general, but also will establish a novel, powerful instrument (Reverse ChIP) to investigate DNA-protein interaction in complex organisms.

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The information about "INTERSPREC" are provided by the European Opendata Portal: CORDIS opendata.

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