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INTERSPREC SIGNED

Investigation of interspecific pollen tube reception

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 INTERSPREC project word cloud

Explore the words cloud of the INTERSPREC project. It provides you a very rough idea of what is the project "INTERSPREC" about.

binding    crosses    animals    screening    glycosylation    differences    fertilization    pollen    reception    lyrata    bacteria    contrast    pts    breeding    y1h    male    identification    dissect    codes    lysm    containing    sequence    gene    correlate    domain    differ    barriers    patterns    almost    chip    light    responsible    reproduction    communication    trans    arabidopsis    plants    release    expression    pave    pointing    glycan    lab    reverse    sperm    tube    accessions    thaliana    mechanism    powerful    player    fungi    symbiotic    acid    amino    pt    gametophyte    cis    play    species    found    biological    regulation    perfectly    causing    symbiosis    female    recognition    manipulating    cell    host    manipulate    interspecific    output    interaction    attest    regulators    organisms    dna    certain    distinction    acting    regulatory    mechanisms    interactions    plant    first    recognize    immunity    preliminary    fundamental    peptide    function    instrument    at5g62150    profile    shed    ways    protein   

Project "INTERSPREC" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITAT ZURICH 

Organization address
address: RAMISTRASSE 71
city: ZURICH
postcode: 8006
website: n.a.

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Total cost 175˙419 €
 EC max contribution 175˙419 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2017
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-07-01   to  2020-06-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITAT ZURICH CH (ZURICH) coordinator 175˙419.00

Map

 Project objective

Cell-cell interaction mechanisms play a role in many fundamental biological processes from symbiotic interactions of fungi and bacteria with their host plants to fertilization both in animals and plants. In plant reproduction, pollen tube (PT) reception is a cell-cell communication process allowing the recognition of the male gametophyte (the PT) by the female gametophyte necessary for fertilization. To investigate this process, the host lab used interspecific crosses between certain Arabidopsis thaliana accessions and A.lyrata pollen, where most PTs are not recognized, causing a failure in sperm release and thus fertilization. In contrast, several other A.thaliana accessions almost perfectly recognize interspecific PTs. Preliminary results identified the gene At5g62150 as a key player for species-specific PT recognition. At5g62150 codes for a LysM-domain containing glycan-binding peptide, pointing at glycosylation patterns for the distinction of intra- and interspecific PTs, a mechanism already known to be important in symbiosis and immunity. It was found that differences in the regulation of At5g62150 in distinct species is responsible for recognition, as the amino acid sequence does not differ between A. thaliana other accessions. With this proposal, we will first attest and manipulate the expression profile of At5g62150 in different accessions to correlate its regulation with its function. Then, we propose to dissect the regulation of At5g62150 in different ways: identification and study of the essential cis-regulatory element for regulation and Y1H screening and Reverse ChIP to find trans-acting regulators. The output will not only pave the way for the understanding and manipulating crosses barriers in plants, which is highly relevant for plant breeding, and shed light on cell-cell communication processes in general, but also will establish a novel, powerful instrument (Reverse ChIP) to investigate DNA-protein interaction in complex organisms.

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The information about "INTERSPREC" are provided by the European Opendata Portal: CORDIS opendata.

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