Opendata, web and dolomites

ItohRibo SIGNED

Structural study on mitochondrial ribosome assembly in human cells

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 ItohRibo project word cloud

Explore the words cloud of the ItohRibo project. It provides you a very rough idea of what is the project "ItohRibo" about.

forming    cas9    combination    hydrophobic    intermediates    encoded    implicated    complexes    mitoribosome    molecules    core    crispr    mitochondria    strength    cryo    stages    respiratory    membrane    tomography    specialized    transient    human    hundreds    trans    cooperation    mitochondrion    populations    82    proteins    nascent    folded    collaborators    assembly    lab    harnessing    rrnas    maturation    nucleoids    gap    et    compartmentalized    em    genome    organelle    expertise    postulated    assembled    intricate    nuclear    transport    mitochondrial    processed    mechanism    expand    microscopy    mitoribosomal    rrna    granules    scope    cascade    biochemical    complemented    localize    formed    resolution    chain    characterization    structural    fill    proven    mature    insights    dynamics    sub    synthesizing    reveal    catalytic    compartments    mt    electron    ribosomal    novo    techniques    mitoribosomes    combining    regulation    class    particle    ribosomes    completely    hierarchical    sections    abundant    exclusively    de    lacking    tight    models    co    single    13    unknown    relies    mechanistic    rna    milieus    heterogeneous    editing   

Project "ItohRibo" data sheet

The following table provides information about the project.

Coordinator
STOCKHOLMS UNIVERSITET 

Organization address
address: UNIVERSITETSVAGEN 10
city: STOCKHOLM
postcode: 10691
website: www.su.se

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Sweden [SE]
 Total cost 173˙857 €
 EC max contribution 173˙857 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2017
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-06-21   to  2020-06-20

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    STOCKHOLMS UNIVERSITET SE (STOCKHOLM) coordinator 173˙857.00

Map

 Project objective

Human mitoribosome represents a distinct class of ribosomes that has specialized in synthesizing exclusively 13 hydrophobic membrane proteins, forming the catalytic core of the respiratory chain. The mature mitoribosome is composed of 82 nuclear encoded proteins and three mt-rRNAs. It is postulated that mitoribosomes are formed in an intricate and well-defined hierarchical process, involving hundreds of proteins and RNA molecules working in cooperation and under tight regulation. However, a structural insight into this process is completely lacking and most of the trans-factors remain unknown. I propose to fill this gap by harnessing CRISPR/Cas9 for genome editing in combination with the state of the art methods in single particle cryo-electron microscopy (cryo-EM). By combining these techniques with biochemical characterization, I will reveal mechanistic insights into how mitoribosomal proteins are assembled in a cascade while nascent mitochondrial rRNA molecules are processed and folded. Since high resolution cryo-EM allows now a unique ability to investigate heterogeneous ribosomal populations and built de novo models, the proposed work will not only reveal the maturation states, but also currently unknown factors implicated in the process. On the other hand, the mitochondrion is compartmentalized into sub-organelle sections such as nucleoids, RNA granules, and membrane-related milieus, and they co-localize with various stages of the mitoribosome assembly. I will also use cryo-electron tomography (cryo-ET) to expand the scope beyond the mitoribosomal complexes and reveal the dynamics of the maturation process and transport mechanism of the assembly intermediates between the sub-organelle compartments. Our approach relies on the most recently developed methodologies and proven strength of the lab complemented by specialized expertise of collaborators, aiming to characterize the transient and low abundant complexes in human mitochondria.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "ITOHRIBO" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "ITOHRIBO" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

PROSPER (2019)

Politics of Rulemaking, Orchestration of Standards, and Private Economic Regulations

Read More  

RAMBEA (2019)

Realistic Assessment of Historical Masonry Bridges under Extreme Environmental Actions

Read More  

RegARcis (2020)

Role of the SWI/SNF complex in the Androgen Receptor cistrome regulation

Read More