Opendata, web and dolomites

MaCChines SIGNED

Molecular machines based on coiled-coil protein origami

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 MaCChines project word cloud

Explore the words cloud of the MaCChines project. It provides you a very rough idea of what is the project "MaCChines" about.

introduce    cage    machines    genes    cages    performing    concatenated    vivo    pioneered    library    2013    structures    de    versatile    toehold    fraction    novo    dimer    structure    frontier    science    pyramid    trigonal    coil    nanomaterials    prism    methodology    contrast    flexible    bipyramid    sequences    cargo    assemble    molecular    robustness    catalysis    shapes    locomotion    regulated    forming    polyhedral    release    folds    demonstrated    offers    fold    molecules    interactions    caging    cc    crosslinked    rewards    designed    linkers    smart    demonstration    modular    pot    functions    pairwise    origami    coiled    tetrahedral    knotted    modules    unseen    types    displacement    throughput    positional    potentials    ccpos    positions    variants    explored    advantages    medicine    sequence    ccpo    disassembly    rearrangement    building    recognition    core    nanotweezers    branch    chain    functional    structural    polypeptide    self    nature    assembles    construction    tiny    proteins    combinatorial    group    protein    compact    hydrophobic    builds    strategy    domains    strategies    single    naturally    assembly   

Project "MaCChines" data sheet

The following table provides information about the project.

Coordinator
KEMIJSKI INSTITUT 

Organization address
address: HAJDRIHOVA 19
city: LJUBLJANA
postcode: 1000
website: http://www.ki.si

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Slovenia [SI]
 Total cost 2˙497˙125 €
 EC max contribution 2˙497˙125 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-ADG
 Funding Scheme ERC-ADG
 Starting year 2018
 Duration (year-month-day) from 2018-09-01   to  2023-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    KEMIJSKI INSTITUT SI (LJUBLJANA) coordinator 2˙362˙838.00
2    EN-FIST CENTER ODLICNOSTI SI (LJUBLJANA) participant 134˙286.00

Map

 Project objective

Proteins are the most versatile and complex smart nanomaterials, forming molecular machines and performing numerous functions from structure building, recognition, catalysis to locomotion. Nature however explored only a tiny fraction of possible protein sequences and structures. Design of proteins with new, in nature unseen shapes and features, offers high rewards for medicine, technology and science. In 2013 my group pioneered the design of a new type of modular coiled-coil protein origami (CCPO) folds. This type of de novo designed proteins are defined by the sequence of coiled-coil (CC) dimer-forming modules that are concatenated by flexible linkers into a single polypeptide chain that self-assembles into a polyhedral cage based on pairwise CC interactions. This is in contrast to naturally evolved proteins where their fold is defined by a compact hydrophobic core. We recently demonstrated the robustness of this strategy by the largest de novo designed single chain protein, construction of tetrahedral, pyramid, trigonal prism and bipyramid cages that self-assemble in vivo. This proposal builds on unique advantages of CCPOs and represents a new frontier of this branch of protein design science. I propose to introduce functional domains into selected positions of CCPO cages, implement new types of building modules that will enable regulated CCPO assembly and disassembly, test new strategies of caging and release of cargo molecules for targeted delivery, design knotted and crosslinked protein cages and introduce toehold displacement for the regulated structural rearrangement of CCPOs required for designed molecular machines, which will be demonstrated on protein nanotweezers. Technology for the positional combinatorial library-based single pot assembly of CCPO genes will provide high throughput of CCPO variants. Project will result in new methodology, understanding of potentials of CCPOs for designed molecular machines and in demonstration of different applications.

 Publications

year authors and title journal last update
List of publications.
2020 Tina Lebar, Duško Lainšček, Estera Merljak, Jana Aupič, Roman Jerala
A tunable orthogonal coiled-coil interaction toolbox for engineering mammalian cells
published pages: , ISSN: 1552-4450, DOI: 10.1038/s41589-019-0443-y
Nature Chemical Biology 2020-03-11
2019 Tina Fink, Jan Lonzarić, Arne Praznik, Tjaša Plaper, Estera Merljak, Katja Leben, Nina Jerala, Tina Lebar, Žiga Strmšek, Fabio Lapenta, Mojca Benčina, Roman Jerala
Design of fast proteolysis-based signaling and logic circuits in mammalian cells
published pages: 115-122, ISSN: 1552-4450, DOI: 10.1038/s41589-018-0181-6
Nature Chemical Biology 15/2 2020-03-11

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "MACCHINES" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "MACCHINES" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

Resonances (2019)

Resonances and Zeta Functions in Smooth Ergodic Theory and Geometry

Read More  

Aware (2019)

Aiding Antibiotic Development with Deep Analysis of Resistance Evolution

Read More  

CARBYNE (2020)

New carbon reactivity rules for molecular editing

Read More