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MaCChines SIGNED

Molecular machines based on coiled-coil protein origami

Total Cost €

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EC-Contrib. €

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Partnership

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 MaCChines project word cloud

Explore the words cloud of the MaCChines project. It provides you a very rough idea of what is the project "MaCChines" about.

modules    naturally    pot    shapes    functional    sequence    variants    origami    domains    positions    performing    library    modular    novo    advantages    coiled    dimer    building    chain    coil    recognition    strategy    pyramid    toehold    medicine    structural    catalysis    prism    rewards    cages    de    sequences    interactions    tetrahedral    linkers    group    potentials    fraction    machines    nanomaterials    knotted    flexible    regulated    branch    disassembly    nature    cc    builds    assemble    core    ccpos    assembles    cargo    protein    types    self    pairwise    polypeptide    ccpo    contrast    structure    smart    tiny    release    compact    construction    folds    versatile    rearrangement    science    molecular    methodology    crosslinked    fold    genes    offers    assembly    proteins    robustness    locomotion    caging    nanotweezers    introduce    vivo    functions    2013    polyhedral    throughput    explored    forming    designed    single    pioneered    concatenated    cage    demonstration    trigonal    combinatorial    displacement    positional    unseen    hydrophobic    structures    strategies    frontier    demonstrated    bipyramid    molecules   

Project "MaCChines" data sheet

The following table provides information about the project.

Coordinator
KEMIJSKI INSTITUT 

Organization address
address: HAJDRIHOVA 19
city: LJUBLJANA
postcode: 1000
website: http://www.ki.si

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Slovenia [SI]
 Total cost 2˙497˙125 €
 EC max contribution 2˙497˙125 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-ADG
 Funding Scheme ERC-ADG
 Starting year 2018
 Duration (year-month-day) from 2018-09-01   to  2023-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    KEMIJSKI INSTITUT SI (LJUBLJANA) coordinator 2˙362˙838.00
2    EN-FIST CENTER ODLICNOSTI SI (LJUBLJANA) participant 134˙286.00

Map

 Project objective

Proteins are the most versatile and complex smart nanomaterials, forming molecular machines and performing numerous functions from structure building, recognition, catalysis to locomotion. Nature however explored only a tiny fraction of possible protein sequences and structures. Design of proteins with new, in nature unseen shapes and features, offers high rewards for medicine, technology and science. In 2013 my group pioneered the design of a new type of modular coiled-coil protein origami (CCPO) folds. This type of de novo designed proteins are defined by the sequence of coiled-coil (CC) dimer-forming modules that are concatenated by flexible linkers into a single polypeptide chain that self-assembles into a polyhedral cage based on pairwise CC interactions. This is in contrast to naturally evolved proteins where their fold is defined by a compact hydrophobic core. We recently demonstrated the robustness of this strategy by the largest de novo designed single chain protein, construction of tetrahedral, pyramid, trigonal prism and bipyramid cages that self-assemble in vivo. This proposal builds on unique advantages of CCPOs and represents a new frontier of this branch of protein design science. I propose to introduce functional domains into selected positions of CCPO cages, implement new types of building modules that will enable regulated CCPO assembly and disassembly, test new strategies of caging and release of cargo molecules for targeted delivery, design knotted and crosslinked protein cages and introduce toehold displacement for the regulated structural rearrangement of CCPOs required for designed molecular machines, which will be demonstrated on protein nanotweezers. Technology for the positional combinatorial library-based single pot assembly of CCPO genes will provide high throughput of CCPO variants. Project will result in new methodology, understanding of potentials of CCPOs for designed molecular machines and in demonstration of different applications.

 Publications

year authors and title journal last update
List of publications.
2020 Tina Lebar, Duško Lainšček, Estera Merljak, Jana Aupič, Roman Jerala
A tunable orthogonal coiled-coil interaction toolbox for engineering mammalian cells
published pages: , ISSN: 1552-4450, DOI: 10.1038/s41589-019-0443-y
Nature Chemical Biology 2020-03-11
2019 Tina Fink, Jan Lonzarić, Arne Praznik, Tjaša Plaper, Estera Merljak, Katja Leben, Nina Jerala, Tina Lebar, Žiga Strmšek, Fabio Lapenta, Mojca Benčina, Roman Jerala
Design of fast proteolysis-based signaling and logic circuits in mammalian cells
published pages: 115-122, ISSN: 1552-4450, DOI: 10.1038/s41589-018-0181-6
Nature Chemical Biology 15/2 2020-03-11

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