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Desiccation Survival SIGNED

Discovery of intrinsically disordered sequences conferring desiccation survival

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 Desiccation Survival project word cloud

Explore the words cloud of the Desiccation Survival project. It provides you a very rough idea of what is the project "Desiccation Survival" about.

desiccation    algorithms    mediated    learned    protect    death    machine    aggregate    idp    designing    screen    discover    unfolding    protection    cell    unravel    form    transition    paradigm    extract    disordered    assay    gelation    protein    shown    perform    validate    ing    rationally    periods    organisms    screening    functions    function    sequences    protective    intracellular    proteins    determinants    underlying    micro    family    materials    tested    library    questions    tardigrades    survival    intrinsically    solids    abundant    question    vitrified    phylum    space    glass    aggregation    rescue    dry    hits    learning    extremely    animals    pipeline    soluble    aid    fundamental    undergo    vitro    confer    sampling    dryness    sequence    biomaterials    vivo    engineering    survive    computational    cahs    biology    heat    unknown    functional    cells    stress    generate    irreversibly    idps    throughput    unfold    cytosolic   

Project "Desiccation Survival" data sheet

The following table provides information about the project.

Coordinator
UNITED KINGDOM RESEARCH AND INNOVATION 

Organization address
address: POLARIS HOUSE NORTH STAR AVENUE
city: SWINDON
postcode: SN2 1FL
website: n.a.

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 212˙933 €
 EC max contribution 212˙933 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2019
 Duration (year-month-day) from 2019-04-01   to  2021-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNITED KINGDOM RESEARCH AND INNOVATION UK (SWINDON) coordinator 212˙933.00

Map

 Project objective

Desiccation is a form of stress wherein extremely dry conditions cause intracellular proteins to unfold and aggregate irreversibly, resulting in cell-death. How do cells and organisms survive desiccation is a fundamental question in biology. Cytosolic Abundant Heat Soluble (CAHS) proteins, a family of intrinsically disordered proteins (IDPs) in tardigrades (a phylum of micro-animals), have been shown to be important for their survival during long periods of dryness. Under desiccation condition, CAHS proteins undergo glass-transition and gelation to form vitrified solids that protect intracellular proteins from unfolding and aggregation. However, the features of CAHS proteins that confer protection are unknown. Here, I aim to unravel the sequence determinants of CAHS protein functions, by addressing 3 specific questions:

Aim 1: What are the sequence features that promote glass-transition and gelation in CAHS proteins? Aim 2: Can we discover new sequences that can rescue cells from desiccation? Aim 3: What is the sequence-to-function paradigm underlying IDP-mediated desiccation survival?

I will (i) perform computational analysis of existing CAHS proteins to extract their sequence features to design a library for adequate sampling of the sequence space; (ii) screen the library with a high-throughput survival-based assay and validate the hits both in vitro and in vivo; (iii) analyse the results with machine learning algorithms to generate characteristic sequence features underlying protective glass-transition. The learned features will be tested by rationally designing and screening a new sequence library for desiccation survival. This project will provide fundamental sequence-level understanding of how IDPs promote stress response, specifically via glass-transition during desiccation. Moreover, the materials and pipeline generated and the findings of this study will aid in engineering functional biomaterials.

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The information about "DESICCATION SURVIVAL" are provided by the European Opendata Portal: CORDIS opendata.

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