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CRISPR-Locate SIGNED

Linking sequence to function of long noncoding RNAs with CRISPR.

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 CRISPR-Locate project word cloud

Explore the words cloud of the CRISPR-Locate project. It provides you a very rough idea of what is the project "CRISPR-Locate" about.

subcellular    extensive    insert    hypothesis    subsequently    characterised    discoveries    encode    protein    estimates    purify    grand    lncrnas    proteins    experimentally    separable    contains    invaluable    conventional    noncoding    time    rnas    delete    unlocking    mature    wake    significance    biological    experiments    alongside    lncrna    modular    encoded    fractionate    mutated    medicine    surprise    endogenous    19    compartments    sequence    molecules    human    resource    created    tagged    laborious    context    create    elucidated    push    place    functional    linking    proxy       tags    primary    responsible    faster    biology    detecting    natural    genes    afterwards    discovering    molecule    handful    pressing    function    least    locate    throughput    deletion    closer    technique    vast    cells    1000    annotation    domains    question    coding    wild       functions    genome    crispr    discovery    presently    efforts    lt    localization    10    rna    simultaneously    map    localisation   

Project "CRISPR-Locate" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITAET BERN 

Organization address
address: HOCHSCHULSTRASSE 6
city: BERN
postcode: 3012
website: http://www.unibe.ch

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Total cost 191˙149 €
 EC max contribution 191˙149 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2019
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2021
 Duration (year-month-day) from 2021-07-01   to  2023-06-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITAET BERN CH (BERN) coordinator 191˙149.00

Map

 Project objective

A great surprise in the wake of the Human Genome Project has been the discovery of vast numbers of RNAs that do not encode proteins. Alongside 19,000 protein-coding genes, our genome contains at least 20,000 long noncoding RNA (lncRNA) genes, but recent estimates push that number to 100,000. Extensive annotation efforts are presently discovering lncRNAs far faster than their functions can be elucidated, and thus only <1% of lncRNAs have been experimentally characterised. These discoveries have created a grand challenge of understanding lncRNAs’ biological significance. To do this, we must solve the pressing question of how lncRNAs’ functions are encoded in their primary sequence. As a proxy of lncRNAs function we can use subcellular localisation since lncRNAs function as a mature RNA molecule. One hypothesis is that, similar to proteins, lncRNAs are modular molecules composed of separable functional domains. Previous studies, including my own, have used conventional methods to identify domains in a handful of lncRNAs by laborious deletion experiments. I propose to advance this field, via a novel high-throughput technique, CRISPR-Locate, capable of detecting lncRNA domains and their function in their natural endogenous context. I will delete ⁓1000 different lncRNA domains at the same time and simultaneously insert RNA tags at their place. Subsequently, I will fractionate cells by their compartments and purify tagged lncRNAs. Afterwards, I will sequence all the tagged lncRNAs and, by comparing the change in the subcellular localisation between mutated and wild type cells, I will identify which domains are responsible for subcellular localization. Finally, I will use CRISPR-Locate to create a map of lncRNA domains, an invaluable resource linking sequence to function, and bring us a step closer to unlocking the potential of 10^4 novel genes in medicine and biology.

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The information about "CRISPR-LOCATE" are provided by the European Opendata Portal: CORDIS opendata.

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