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Wall-E SIGNED

Make it, don’t break it: a reconstitution screen for Casparian strip formation in the root endodermis

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 Wall-E project word cloud

Explore the words cloud of the Wall-E project. It provides you a very rough idea of what is the project "Wall-E" about.

lethality    expressing    reconstitute    efficiency    impregnations    wp1    machinery    function    wp3    selective    genes    barrier    root    wall    laccases    assembly    oxide    minimally    slowly    limitations    cell    cellular    differentiation    wp2    stability    proteins    master    attempt    pioneering    reverse    crispr    super    enzymes    causing    peroxidases    developmental    decade    dependent    formed    secondly    nutrients    polymerising    plants    activating    redundancy    last    functions    cells    model    introduce    firstly    activation    coming    gene    endodermal    dismutases    thirdly    uncover    standstill    genetic    generation    players    regulator    screening    gain    activator    myb36    organisms    faced    strips    screen    combinatorial    mutant    assessing    arabidopsis    pleiotropy    molecular    differentiating    water    pioneer    core    mechanisms    dirigent    elucidate    lignin    endodermis    genetically    transmembrane    casparian    sufficient    cs   

Project "Wall-E" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITE DE LAUSANNE 

Organization address
address: Quartier Unil-Centre Bâtiment Unicentre
city: LAUSANNE
postcode: 1015
website: www.unil.ch

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Total cost 191˙149 €
 EC max contribution 191˙149 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2019
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2021
 Duration (year-month-day) from 2021-08-01   to  2023-07-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITE DE LAUSANNE CH (LAUSANNE) coordinator 191˙149.00

Map

 Project objective

In plants, the root endodermis functions as a barrier, allowing the selective uptake of nutrients and water. The barrier is formed by cell wall impregnations called Casparian strips (CS), produced by differentiating endodermal cells. In the last decade, pioneering work on the endodermis has identified numerous players involved in CS formation, including transmembrane proteins, peroxidases, dirigent-like proteins, lignin polymerising enzymes, laccases and super-oxide dismutases. However, the forward and reverse genetic approaches used to uncover these proteins are slowly coming to a standstill due to their limitations when faced with gene redundancy or genes with a broader range of activities, causing pleiotropy or lethality. I now propose to use CS formation as a model in order to pioneer a combinatorial, gain-of-function screen, aiming to define a minimally sufficient gene set for the assembly of a CS. I will attempt to reconstitute a CS in the endodermis of the myb36 mutant – a master regulator of endodermal differentiation - by activation screening for genes within the MYB36-dependent gene set. Firstly, I will introduce a “core machinery” for CS formation by expressing important known players and assessing CS formation and stability (WP1). Secondly, I will test the most recently developed second generation CRISPR activator systems for their efficiency in activating genes of interest in the endodermis (WP2). Thirdly, using the CRISPR activator technology, I will screen for novel genes that will improve the formation, stability and functionality of the CS (WP3). Identifying new genes involved in CS formation through a combinatorial, gain-of-function approach represents a novel way to genetically elucidate molecular mechanisms and could become a model for other cellular and developmental processes in Arabidopsis or other organisms.

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The information about "WALL-E" are provided by the European Opendata Portal: CORDIS opendata.

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