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Wall-E SIGNED

Make it, don’t break it: a reconstitution screen for Casparian strip formation in the root endodermis

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 Wall-E project word cloud

Explore the words cloud of the Wall-E project. It provides you a very rough idea of what is the project "Wall-E" about.

secondly    gene    minimally    nutrients    organisms    enzymes    genetically    model    developmental    machinery    wp3    wall    water    strips    causing    endodermis    cell    firstly    limitations    transmembrane    mechanisms    wp2    molecular    master    genetic    lethality    super    combinatorial    oxide    attempt    functions    casparian    myb36    wp1    reconstitute    screen    sufficient    differentiating    differentiation    activating    plants    efficiency    arabidopsis    coming    activation    redundancy    faced    decade    reverse    thirdly    pleiotropy    barrier    stability    root    cells    mutant    peroxidases    introduce    assembly    dependent    dirigent    players    endodermal    core    selective    polymerising    gain    activator    standstill    cs    formed    lignin    pioneer    function    screening    regulator    generation    dismutases    pioneering    cellular    laccases    slowly    uncover    elucidate    expressing    impregnations    assessing    crispr    last    genes    proteins   

Project "Wall-E" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITE DE LAUSANNE 

Organization address
address: Quartier Unil-Centre Bâtiment Unicentre
city: LAUSANNE
postcode: 1015
website: www.unil.ch

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Total cost 191˙149 €
 EC max contribution 191˙149 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2019
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2021
 Duration (year-month-day) from 2021-08-01   to  2023-07-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITE DE LAUSANNE CH (LAUSANNE) coordinator 191˙149.00

Map

 Project objective

In plants, the root endodermis functions as a barrier, allowing the selective uptake of nutrients and water. The barrier is formed by cell wall impregnations called Casparian strips (CS), produced by differentiating endodermal cells. In the last decade, pioneering work on the endodermis has identified numerous players involved in CS formation, including transmembrane proteins, peroxidases, dirigent-like proteins, lignin polymerising enzymes, laccases and super-oxide dismutases. However, the forward and reverse genetic approaches used to uncover these proteins are slowly coming to a standstill due to their limitations when faced with gene redundancy or genes with a broader range of activities, causing pleiotropy or lethality. I now propose to use CS formation as a model in order to pioneer a combinatorial, gain-of-function screen, aiming to define a minimally sufficient gene set for the assembly of a CS. I will attempt to reconstitute a CS in the endodermis of the myb36 mutant – a master regulator of endodermal differentiation - by activation screening for genes within the MYB36-dependent gene set. Firstly, I will introduce a “core machinery” for CS formation by expressing important known players and assessing CS formation and stability (WP1). Secondly, I will test the most recently developed second generation CRISPR activator systems for their efficiency in activating genes of interest in the endodermis (WP2). Thirdly, using the CRISPR activator technology, I will screen for novel genes that will improve the formation, stability and functionality of the CS (WP3). Identifying new genes involved in CS formation through a combinatorial, gain-of-function approach represents a novel way to genetically elucidate molecular mechanisms and could become a model for other cellular and developmental processes in Arabidopsis or other organisms.

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