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Wall-E SIGNED

Make it, don’t break it: a reconstitution screen for Casparian strip formation in the root endodermis

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 Wall-E project word cloud

Explore the words cloud of the Wall-E project. It provides you a very rough idea of what is the project "Wall-E" about.

nutrients    elucidate    crispr    activation    organisms    wp1    faced    peroxidases    causing    wall    arabidopsis    mutant    attempt    minimally    sufficient    molecular    plants    standstill    barrier    gene    water    activator    pioneering    genes    expressing    players    differentiating    myb36    screening    root    polymerising    cs    firstly    slowly    efficiency    wp2    generation    endodermis    activating    regulator    endodermal    developmental    lethality    pioneer    coming    model    gain    cell    last    uncover    cells    mechanisms    lignin    dismutases    core    secondly    enzymes    stability    formed    limitations    cellular    impregnations    reverse    screen    differentiation    redundancy    selective    assessing    dirigent    transmembrane    thirdly    casparian    master    oxide    genetically    function    decade    assembly    machinery    laccases    dependent    strips    functions    pleiotropy    genetic    reconstitute    super    combinatorial    introduce    proteins    wp3   

Project "Wall-E" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITE DE LAUSANNE 

Organization address
address: Quartier Unil-Centre Bâtiment Unicentre
city: LAUSANNE
postcode: 1015
website: www.unil.ch

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Total cost 191˙149 €
 EC max contribution 191˙149 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2019
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2021
 Duration (year-month-day) from 2021-08-01   to  2023-07-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITE DE LAUSANNE CH (LAUSANNE) coordinator 191˙149.00

Map

 Project objective

In plants, the root endodermis functions as a barrier, allowing the selective uptake of nutrients and water. The barrier is formed by cell wall impregnations called Casparian strips (CS), produced by differentiating endodermal cells. In the last decade, pioneering work on the endodermis has identified numerous players involved in CS formation, including transmembrane proteins, peroxidases, dirigent-like proteins, lignin polymerising enzymes, laccases and super-oxide dismutases. However, the forward and reverse genetic approaches used to uncover these proteins are slowly coming to a standstill due to their limitations when faced with gene redundancy or genes with a broader range of activities, causing pleiotropy or lethality. I now propose to use CS formation as a model in order to pioneer a combinatorial, gain-of-function screen, aiming to define a minimally sufficient gene set for the assembly of a CS. I will attempt to reconstitute a CS in the endodermis of the myb36 mutant – a master regulator of endodermal differentiation - by activation screening for genes within the MYB36-dependent gene set. Firstly, I will introduce a “core machinery” for CS formation by expressing important known players and assessing CS formation and stability (WP1). Secondly, I will test the most recently developed second generation CRISPR activator systems for their efficiency in activating genes of interest in the endodermis (WP2). Thirdly, using the CRISPR activator technology, I will screen for novel genes that will improve the formation, stability and functionality of the CS (WP3). Identifying new genes involved in CS formation through a combinatorial, gain-of-function approach represents a novel way to genetically elucidate molecular mechanisms and could become a model for other cellular and developmental processes in Arabidopsis or other organisms.

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