PEPSTEM

Effect of neuropeptides on selective neuronal differentiation of mouse embryonic stem cells

Coordinatore	BIOTALENTUM TUDASFEJLESZTO KFT    more  Organization address address: AULICH LAJOS UTCA 26 city: GOEDOELLO postcode: 2100 contact info Nome: Ocsovszky Cognome: Zsofia Email: send email Telefono: +36 202750933 Fax: 3628526243
Nazionalità Coordinatore	Hungary [HU]
Totale costo	45˙000 €
EC contributo	45˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2010-RG
Funding Scheme	MC-ERG
Anno di inizio	2012
Periodo (anno-mese-giorno)	2012-05-21   -   2015-05-20

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	BIOTALENTUM TUDASFEJLESZTO KFT  Organization address address: AULICH LAJOS UTCA 26 city: GOEDOELLO postcode: 2100 contact info Nome: Ocsovszky Cognome: Zsofia Email: send email Telefono: +36 202750933 Fax: 3628526243	HU (GOEDOELLO)	coordinator	45˙000.00

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 Obiettivo del progetto (Objective)

'The capacity of embryonic stem cells (ES cells) to differentiate into neuronal cells represents a potential source for neuronal replacement and a model for studying factors controlling early stages of neuronal differentiation. Various molecules have been used to induce such differentiation but so far neuropeptides acting via G-protein coupled receptors have not yet been profoundly investigated. Since undifferentiated ES-cells express numerous G-coupled receptors which expression is increasing with time in culture, it suggest a prominent role of these receptors in the maintenance and development of ES cells. RT-PCR analyses of Ghrelin is proven to have a prominent effect on the growth and proliferation of the neural progenitor cells in the dorsal motor nucleus of the vagus (DMNV), but its effect on ES cells has not yet been investigated. MC4 receptor, which binds both alpha-MSH and AgRP is expressed by ES-cells but wheter it has a function on neuronal differentiation is unknown. Y1 and Y5 receptors of NPY have proven to have a significant role in maintaining the pluripotency of ES cells. These original data demonstrate that functional G-protein coupled receptors have a role on differentiation into a neuronal phenotype. It opens an exciting new field for neuropeptide regulation of tissue ontogenesis. Using a three-dimensional „minibrain” could reveal potential selective differentiation in the forming tissue. Since these neuropeptides often regulates each other’s expression in the adult brain, it would be interesting to see whether these regulation networks can form in vitro in a differentiating cell-culture. This would open new ways of studying the effect of neuropeptides on neural differentiation and synaptic network formation.'