NCCHROMICS

Mechanisms underlying epigenetic regulation by small non-coding RNAs

Coordinatore	EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZURICH    more  Organization address address: Raemistrasse 101 city: ZUERICH postcode: 8092 contact info Titolo: Prof. Nome: Renato Cognome: Paro Email: send email Telefono: +41 61 387 31 20 Fax: +41 61 387 39 96
Nazionalità Coordinatore	Switzerland [CH]
Totale costo	275˙362 €
EC contributo	275˙362 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2011-IOF
Funding Scheme	MC-IOF
Anno di inizio	0
Periodo (anno-mese-giorno)	0000-00-00   -   0000-00-00

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZURICH  Organization address address: Raemistrasse 101 city: ZUERICH postcode: 8092 contact info Titolo: Prof. Nome: Renato Cognome: Paro Email: send email Telefono: +41 61 387 31 20 Fax: +41 61 387 39 96	CH (ZUERICH)	coordinator	275˙362.50

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 Obiettivo del progetto (Objective)

'For a long time it has been accepted that a big part of the genome consisted of the so-called 'junk' DNA without functional importance. Through the ENCODE project it has been evident that the vast majority of this DNA is in fact transcribed into RNA, producing a huge number of small and long non-coding RNAs (ncRNAs) of unknown function. Previous studies suggested that ncRNAs may be implicated among others in epigenetic regulation. Aim of the current project is to investigate the mechanisms underlying this form of regulation with regards to gene silencing by Polycomb Repression Complex 2 (PRC2) and establishment of specific chromatin marks like H4K12 acetylation. As a model system we employ mouse pluripotent stem cells during differentiation and reprogramming, which are characterized by remarkable chromatin remodelling offering the possibility to track chromatin dynamics in relation with changes in ncRNAs. We will apply RIP-seq for PRC2 to identify small ncRNAs associated with action of this complex and test how they are connected with silencing of selected genomic regions in our model system. To this end, we want to identify the mechanisms through which these ncRNAs guide PRC2. In addition, we want to develop a high throughput approach to map genome wide at the chromatin level small ncRNAs associated with selected histone modifications like H4K12 acetylation and check their distribution around critical genomic elements like promoters and splice junctions. Deliverables of this project will provide an unprecedented insight into the way chromatin modifications and complexes are involved in epigenetic regulation via ncRNAs. This project combines the field of epigenetics with the emerging field of ncRNAs. It will enable the applicant to interact with a lab in Harvard which is world leader in the field of ncRNAs and a lab in ETH Zurich which is driving force in epigenetics, thus creating interesting synergies between the two research environments towards RNAepigenetics.'