POSTSCRIBEDNADAMAGE

Global characterization of post-transcriptional regulatory interactions in DNA damage response

 Coordinatore MAX-DELBRUCK-CENTRUM FUR MOLEKULARE MEDIZIN IN DER HELMHOLTZ-GEMEINSCHAFT 

 Organization address address: ROBERT ROSSLE STRASSE 10
city: BERLIN
postcode: 13125

contact info
Titolo: Dr.
Nome: Ioannis
Cognome: Legouras
Email: send email
Telefono: +49 30940644247
Fax: 4930940000000

 Nazionalità Coordinatore Germany [DE]
 Totale costo 174˙475 €
 EC contributo 174˙475 €
 Programma FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call FP7-PEOPLE-2011-IEF
 Funding Scheme MC-IEF
 Anno di inizio 2012
 Periodo (anno-mese-giorno) 2012-07-01   -   2014-06-30

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    MAX-DELBRUCK-CENTRUM FUR MOLEKULARE MEDIZIN IN DER HELMHOLTZ-GEMEINSCHAFT

 Organization address address: ROBERT ROSSLE STRASSE 10
city: BERLIN
postcode: 13125

contact info
Titolo: Dr.
Nome: Ioannis
Cognome: Legouras
Email: send email
Telefono: +49 30940644247
Fax: 4930940000000

DE (BERLIN) coordinator 174˙475.20

Mappa


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rna    candidates    post    protein    ddr    transcriptional    dynamics    biology    biochemical    mrna    cells    cell    proteins    global    translational    dna    regulation    tumour   

 Obiettivo del progetto (Objective)

'The proposed project aims to identify the dynamics of RNA-protein interactions during DNA damage response (DDR) using high-throughput “omics” approaches. The mechanistic basis of DDR has been classically thought to consist of an 'early' post-translational response, which results in activation of several signalling pathways and transciption factors. These activate the 'late' transcriptional reprogramming influencing DNA repair, cell cycle regulation and apoptosis. However, for an immediate and effective response, global post-transcriptional control of mRNA stability, splicing and translation may be crucial during “immediate-to-early” DDR. In this project, I will employ biochemical, cell, systems biology and computational approaches to study the relevance of post-transcriptional regulators such as RNA-binding proteins and microRNAs in DDR. Quantitative proteomics will be applied to identify the differentially mRNA-bound proteins after the exposure of human cultured cells to ionizing radiation. Validation of protein candidates will be carried out by biochemical and deep sequencing approaches. In addition, I will characterize the transcriptome-wide response of existing RNA molecules as well as the newly synthesized transcripts. This will provide a global insight into the dynamics of RNAs and proteins during DDR. The proposed project explores a previously uncharacterized link between post-transcriptional RNA operons and DDR. The results and implementations of this project will significantly contribute to the understanding of the regulation of DDR, as well as have potential clinical and translational impact. Novel tumour-specific RNA-protein interacting partners may serve as candidates to selectively induce cell death in tumour cells. The proposed project will enable a focused career development period in the scope of European mobility for the researcher who will gain a high level of expertise in systems biology and establish several international collaborations.'

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