MEREMY6

Uncovering the mechanism and physiological relevance of myosin-VI-dependent AMPA receptor trafficking in neurons

Coordinatore	more  Organization address address: Martinistrasse 52 city: HAMBURG postcode: 20246 contact info Nome: Uwe Cognome: Csizmadia-Barth Email: send email Telefono: +49 40 741055188 Fax: +49 40 741056261
Nazionalità Coordinatore	Non specificata
Totale costo	100˙000 €
EC contributo	100˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Anno di inizio	2012
Periodo (anno-mese-giorno)	2012-08-01   -   2016-07-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	UNIVERSITAETSKLINIKUM HAMBURG-EPPENDORF  Organization address address: Martinistrasse 52 city: HAMBURG postcode: 20246 contact info Nome: Uwe Cognome: Csizmadia-Barth Email: send email Telefono: +49 40 741055188 Fax: +49 40 741056261	DE (HAMBURG)	coordinator	100˙000.00

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 Obiettivo del progetto (Objective)

'The proper trafficking of membranes and associated proteins in neurons is crucial for the function of the nervous system. Myosin-VI is an actin-based cytoskeletal motor that promotes membrane trafficking and is suggested to play crucial roles in neurons. Most notably, the myosin affects the intracellular trafficking of AMPA receptors, major neurotransmitter receptors composed of transmembrane protein subunits. In addition, data suggest a potential link between myosin-VI and neurodegenerative diseases such as Alzheimer’s. Here, we want to dissect the molecular mechanism by which myosin-VI acts in AMPA receptor trafficking in neurons. Furthermore, we aim to determine the relevance of myosin-VI-driven AMPA receptor trafficking events for structure, function and plasticity of a well-characterized neuronal circuit, and for behavioral output. To this end, we will focus on Purkinje neurons as a model system. These cerebellar neurons are crucial for the coordination and fine-tuning of movements and for certain forms of motor learning. We will make use of genetically altered mice in combination with approaches such as transfection and live cell microscopy of cultured Purkinje neurons, in vitro genetic rescue experiments, ultra-structural analyses via electron microscopy, electrophysiological methods and behavioral tests. We expect that our study will provide novel, general insights into the mechanism by which myosin-VI functions in membrane trafficking. In particular, the results should shed new light onto the molecular and cellular events that take place in Purkinje neurons and determine output from the cerebellum. Moreover, understanding the mechanism of function of neuronal myosin-VI might impact our view of the pathogenesis of neurodegenerative disease.'