NUCLEAR PORE

The nuclear pore permeability barrier – physical concepts and a biosynthetic approach to understand and exploit the unique selectivity of a natural macromolecular sieve

 Coordinatore ASOCIACION CENTRO DE INVESTIGACION COOPERATIVA EN BIOMATERIALES 

 Organization address address: PASEO MIRAMON PARQUE TECNOLOGICO DE SAN SEBASTIAN EDIFICIO EMPRESARIAL C 182
city: SAN SEBASTIAN
postcode: 20009

contact info
Titolo: Mr.
Nome: Alfonso
Cognome: Egaña
Email: send email
Telefono: +34 943 005 300
Fax: +34 943 005 301

 Nazionalità Coordinatore Spain [ES]
 Totale costo 166˙336 €
 EC contributo 166˙336 €
 Programma FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call FP7-PEOPLE-2012-IEF
 Funding Scheme MC-IEF
 Anno di inizio 2013
 Periodo (anno-mese-giorno) 2013-03-01   -   2015-02-28

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    ASOCIACION CENTRO DE INVESTIGACION COOPERATIVA EN BIOMATERIALES

 Organization address address: PASEO MIRAMON PARQUE TECNOLOGICO DE SAN SEBASTIAN EDIFICIO EMPRESARIAL C 182
city: SAN SEBASTIAN
postcode: 20009

contact info
Titolo: Mr.
Nome: Alfonso
Cognome: Egaña
Email: send email
Telefono: +34 943 005 300
Fax: +34 943 005 301

ES (SAN SEBASTIAN) coordinator 166˙336.20

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

barrier    nuclear    supramolecular    vitro    molecular    ntrs    selective    domains    biological    transport    assemblies    species    fg    model    bound    pore    repeat    permeability    selectivity   

 Obiettivo del progetto (Objective)

'Nuclear pore complexes (NPCs) are the exclusive gate of entry/exit of macromolecules into and out of the nucleus of living cells. They exhibit a unique selectivity in transport, with respect to size and molecular species: very small molecules can diffuse efficiently through the pore, while larger objects are delayed/blocked unless they are bound to specialized proteins, so called nuclear transport receptors (NTRs). The permeability barrier and its selectivity arise from an assembly of protein domains that are rich in phenylalanine-glycine repeats (FG repeat domains). These domains are natively unfolded and grafted at high density to the NPC channel walls. NTRs can interact with the FG repeat domains, thereby facilitating translocation of NTR-bound cargo.

The supramolecular organization of the nuclear pore permeability barrier and the mechanism behind selective transport remain insufficiently understood. Our goal is to understand the relation between the organizational and dynamic features of FG-nucleoporin assemblies, their physicochemical properties, and the resulting biological functions. Using an approach that combines tailor-made biomimetic in vitro model systems, surface-sensitive in situ analysis techniques, and polymer physics theory, we will quantitatively study these relationships on the supramolecular level, a level that – for this type of assemblies – is hardly accessible with conventional biological and biophysical approaches. The model systems will be well-defined allowing tight control over composition and supramolecular structure. In a second step, we will apply the obtained knowledge and construct a biosynthetic in vitro system that performs active, directed macromolecular transport.

If successful, this highly interdisciplinary project will greatly increase our mechanistic understanding of nuclear transport. It will also enable the development of novel biotechnology such as species-selective molecular separation devices.'

Altri progetti dello stesso programma (FP7-PEOPLE)

COMVEBONOV (2008)

Computational Modelling and Analysis of Automotive Vehicle Body Noise and Vibration

Read More  

PERIODS (2009)

Periods of modular forms

Read More  

NFESEC (2012)

Nanophotonics for Efficient Solar-to-H2 Energy Conversion

Read More