NANODYNATCELLVATION

Nano -structural and -dynamic events in the T-cell activation

 Coordinatore THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD 

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 Nazionalità Coordinatore United Kingdom [UK]
 Totale costo 100˙000 €
 EC contributo 100˙000 €
 Programma FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call FP7-PEOPLE-2013-CIG
 Funding Scheme MC-CIG
 Anno di inizio 2013
 Periodo (anno-mese-giorno) 2013-09-01   -   2017-08-31

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD

 Organization address address: University Offices, Wellington Square
city: OXFORD
postcode: OX1 2JD

contact info
Titolo: Dr.
Nome: Stephen
Cognome: Conway
Email: send email
Telefono: +44 1865 289800
Fax: +44 1865 289801

UK (OXFORD) coordinator 100˙000.00
2    SCIENCE AND TECHNOLOGY FACILITIES COUNCIL

 Organization address address: Polaris House North Star Avenue
city: SWINDON
postcode: SN2 1SZ

contact info
Titolo: Mr.
Nome: Richard
Cognome: Glover
Telefono: 441235000000
Fax: 4412355303

UK (SWINDON) coordinator 0.00
3    THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD

 Organization address address: University Offices, Wellington Square
city: OXFORD
postcode: OX1 2JD

contact info
Titolo: Dr.
Nome: Stephen
Cognome: Conway
Email: send email
Telefono: +44 1865 289800
Fax: +44 1865 289801

UK (OXFORD) participant 100˙000.00

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

immunology    triggering    nanoscale    cells    molecules    interactions    immunological    constitution    cell    fluorescence    resolution    follow    membrane    specifically    super    molecular    synapse    fcs    sted    resting    microscopy   

 Obiettivo del progetto (Objective)

The organization of the T-cell in its resting and activated state, specifically of plasma-membrane molecules involved in the triggering of the T-cell, is a long-standing and contentious research topic in molecular immunology. Understanding the molecular mechanisms involved at the nanoscale, i.e. at spatial scales below the resolution limit of conventional optical microscopy (< 200 nm) will find answers to still open questions, and offer new routes to immunotherapy. The proposed multidisciplinary project aims to bring close together super-resolution STED microscopy and membrane biophysics with molecular immunology. The proposed studies will follow a holistic approach and include the investigation of the functional associations of a multitude of molecules in T-cell triggering, ranging from the T-cell receptor and co-receptors, over other proteins such as kinases and phosphatases, to lipids and the cortical cytoskeleton. Using super-resolution STED and single-molecule microscopy (specifically STED and fluorescence correlation spectroscopy, STED-FCS) I will directly observe, determine, follow and evaluate nanoscale molecular interactions of these molecules from the resting state and early activation of the T-cell until the constitution of the immunological synapse. Besides a sophisticated design of the experimental conditions such as appropriate live-cell fluorescence labeling, biochemical treatments and choice of activating the T-cell, I will improve the STED-FCS technology towards multi-color observations allowing the determination of nanoscale dynamics and interactions of different specific molecules at the same time. With new molecular interactions highlighted, I will be able to better understand how T-cells sense antigen presenting cells and what molecular ramifications are involved during the constitution of the immunological synapse.

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