PROTCAGE

Chemistry in the Confinement of Protein Cages

 Coordinatore UNIVERSITEIT TWENTE 

Spiacenti, non ci sono informazioni su questo coordinatore. Contattare Fabio per maggiori infomrazioni, grazie.

 Nazionalità Coordinatore Netherlands [NL]
 Totale costo 1˙994˙400 €
 EC contributo 1˙994˙400 €
 Programma FP7-IDEAS-ERC
Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call ERC-2013-CoG
 Funding Scheme ERC-CG
 Anno di inizio 2014
 Periodo (anno-mese-giorno) 2014-05-01   -   2019-04-30

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    UNIVERSITEIT TWENTE

 Organization address address: DRIENERLOLAAN 5
city: ENSCHEDE
postcode: 7522 NB

contact info
Titolo: Mr.
Nome: Ferdinand
Cognome: Damhuis
Email: send email
Telefono: +31 534894019
Fax: +31 534894841

NL (ENSCHEDE) hostInstitution 1˙994˙400.00
2    UNIVERSITEIT TWENTE

 Organization address address: DRIENERLOLAAN 5
city: ENSCHEDE
postcode: 7522 NB

contact info
Titolo: Prof.
Nome: Jeroen Johannes Lambertus Maria
Cognome: Cornelissen
Email: send email
Telefono: +31 534894380
Fax: +31 534894645

NL (ENSCHEDE) hostInstitution 1˙994˙400.00

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

us    cell    protein    cells    encapsulins    enzymes    cages    chemical    organisms    inside    conversions    formed    bacterial    artificial    organelles    probes   

 Obiettivo del progetto (Objective)

'Protein cages appear to be common structures in biology, found in viruses but also in organelle-like containers discovered in bacteria. In this proposed program I aim to study chemical processes in nano-sized protein cages as mimics of bacterial organelles and to increase the general understanding of chemistry in confinement. Towards this goal we will investigate the controlled in vivo loading of bacterial protein cages, i.e. encapsulins, with proteins and enzymes. This will allow us to study in detail the chemical conversions that take place inside such capsules and it will increase understanding about the reasons why certain processes inside these simple organisms are encased in the protein organelles. Completely artificial protein organelles will be constructed by in vitro processes using the well-studied Cowpea Chlorotic Mottle virus cage. By employing DNA technology, cages will be loaded with a single enzyme, a sequence of enzymes or molecular probes. By obtaining this high level of control, we can not only study chemical conversions on the inside, but it will also allow us to monitor the physiochemical properties, such as internal pH, polarity and porosity of the protein mantle by encasing the relevant probes or host/guest systems. In the ultimate stage of the proposed project the formed artificial organelles will be brought into cells in order to interact with the cell metabolism. CCMV has to be introduced by surface modification, while encapsulins can be formed inside these cells; albeit with different cargo. Such experiments have, to my knowledge, not been carried out and introducing new reactions inside these organisms can lead to new potentially interesting products or interfere with cell vitality. The latter can be of importance for the controlled disruption of bacterial cells.'

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SUPOCOSYS (2010)

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FREECO (2012)

Freezing Colloids

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EXPANDERS (2008)

Expander Graphs in Pure and Applied Mathematics

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